Bibliographic Details
| Title: |
Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. |
| Authors: |
Lin, Shin‐e1, Barrette, Anne Marie1, Chapin, Cheryl1, Gonzales, Linda W.2, Gonzalez, Robert F.3, Dobbs, Leland G.3, Ballard, Philip L.1 |
| Source: |
Physiological Reports. Dec2015, Vol. 3 Issue 12, pn/a-N.PAG. 19p. |
| Subject Terms: |
*CARCINOEMBRYONIC antigen, *TUMOR antigens, *CELL adhesion, *CELL communication, *PROGENITOR cells |
| Abstract: |
Carcinoembryonic antigen-related cell adhesion molecule 6 ( CEACAM6) is expressed in the epithelium of various primate tissues, including lung airway and alveoli. In human lung, CEACAM6 is developmentally and hormonally regulated, protects surfactant function, has anti-apoptotic activity and is dysregulated in cancers. We hypothesized that alveolar CEACAM6 expression increases in lung injury and promotes cell proliferation during repair. Studies were performed in CEABAC transgenic mice-containing human CEACAM genes. The level of CEACAM6 in adult CEABAC lung was comparable to that in human infants; expression occurred in epithelium of airways and of some alveoli but rarely co-localized with markers of type I or type II cells. Ten days after bleomycin instillation, both the number of CEACAM6+ cells and immunostaining intensity were elevated in injured lung areas, and there was increased co-localization with type I and II cell markers. To specifically address type II cells, we crossed CEABAC mice with animals expressing EGFP driven by the SP-C promoter. After bleomycin injury, partially flattened, elongated epithelial cells were observed that expressed type I cell markers and were primarily either EGFP+ or CEACAM6+. In cell cycle studies, mitosis was greater in CEACAM6+ non-type II cells versus CEACAM6+/ EGFP+ cells. CEACAM6 epithelial expression was also increased after hyperoxic exposure and LPS instillation, suggesting a generalized response to acute lung injuries. We conclude that CEACAM6 expression is comparable in human lung and the CEABAC mouse. CEACAM6 in this model appears to be a marker of a progenitor cell population that contributes to alveolar epithelial cell replenishment after lung injury. [ABSTRACT FROM AUTHOR] |
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