Bibliographic Details
| Title: |
Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays. |
| Authors: |
Ghorbani, Sarieh1,2, Yao-Cheng Lin1,2, Parizot, Boris1,2, Fernandez, Ana1,2, Njo, Maria Fransiska1,2, de Peer, Yves Van1,2,3, Beeckman, Tom1,2, Hilson, Pierre1,2,4 pierre.hilson@versailles.inra.fr |
| Source: |
Journal of Experimental Botany. Aug2015 Special Issue, Vol. 66 Issue 17, p5257-5269. 13p. |
| Subject Terms: |
*PLANT genomes, *ROOT development, *PEPTIDE analysis, *CELL communication, *ARABIDOPSIS thaliana genetics, *PLANT phylogeny, *NUCLEOTIDE sequencing, *GENETIC transcription in plants |
| Abstract: |
Plant genomes encode numerous small secretory peptides (SSPs) whose functions have yet to be explored. Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication. Because genome annotation based on short sequence homology is difficult, the criteria for the de novo identification and aggregation of conserved SSP sequences were first benchmarked across five reference plant species. The resulting gene families were then extended to 32 genome sequences, including major crops. The global phylogenetic pattern common to the functionally characterized SSP families suggests that their apparition and expansion coincide with that of the land plants. The SSP families can be searched online for members, sequences and consensus (http://bioinformatics. psb.ugent.be/webtools/PlantSSP/). Looking for putative regulators of root development, Arabidopsis thaliana SSP genes were further selected through transcriptome meta-analysis based on their expression at specific stages and in specific cell types in the course of the lateral root formation. As an additional indication that formerly uncharacterized SSPs may control development, this study showed that root growth and branching were altered by the application of synthetic peptides matching conserved SSP motifs, sometimes in very specific ways. The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms. A similar approach can be implemented in different species for the study of a wide range of developmental programmes. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Experimental Botany is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
Full Text from JSTOR