| Title: |
The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain. |
| Authors: |
Tran, Lucile (AUTHOR), Judor, Jean-Paul (AUTHOR), Gauttier, Vanessa (AUTHOR), Geist, Michel (AUTHOR), Hoffman, Chantal (AUTHOR), Rooke, Ronald (AUTHOR), Vassaux, Georges (AUTHOR), Conchon, Sophie (AUTHOR) |
| Source: |
Journal of Biomedicine & Biotechnology. 2012, Vol. 2012, p1-7. 7p. |
| Abstract: |
Aim. To investigate the ability of recombinant modified vaccinia virus Ankara (rMVA) vector to induce an immune response against a well-tolerated self-antigen. Methods. rMVA vectors expressing different form of α-fetoprotein (AFP) were produced and characterized. Naïve mice were vaccinated with MVA vectors expressing the AFP antigen in either a secreted, or a membranebound, or an intracellular form. The immune response was monitored by an IFNγ ELISpot assay and antibody detection. Results. Vaccination with the membrane-associated form of AFP induced a stronger CD8+ T-cell response compared to the ones obtained with the MVA encoding the secreted or the intracellular forms of AFP. Moreover, the vaccination with the membrane-bound AFP elicited the production of AFP-specific antibodies. Conclusions. The AFP transmembrane form is more immunogenic. Expressing a membrane-bound form in the context of an MVA vaccination could enhance the immunogenicity of a self-antigen. [ABSTRACT FROM AUTHOR] |
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| URL: |
85115453 |
| Database: |
Academic Search Complete |
