| Title: |
Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line. |
| Authors: |
HAN LEI1, DALI FENG1, FUXIANG ZHOU1,2 happyzhoufx@sina.com, HUI XU1, TIAN TANG1, HAIJUN YU1,2, CONGHUA XIE1,2, YUNFENG ZHOU1,2 |
| Source: |
Oncology Letters. Aug2015, Vol. 10 Issue 2, p1149-1154. 6p. |
| Subject Terms: |
*CANCER treatment, *RADIOTHERAPY, *TELOMERES, *CELL death, *GENE expression |
| Abstract: |
The close association between telomere length and radiosensitivity has been established by several studies. There is also a hypothesis that telomere length may be regulated by human protection of telomere 1 (hPOT1) in human carcinoma cells. In the present study, the hPOT1 level between the radio-resistant Hep-2R cells and the wild-type were compared, and the results showed that the hPOT1 gene was upregulated in the radioresistant Hep-2R cell lines compared with the wild-type. This suggested that the expression level of hPOT1 correlates with radiosensitivity. Additionally, an hPOT1-directed short hairpin (sh)RNA plasmid was constructed and transferred into the Hep-2R cells, which lead to telomere shortening, an increase in apoptosis and markedly decreased growth of the RNAi-Hep-2R cell line. These results demonstrate that hPOT1-directed shRNAs are associated with telomere length and radiosensitivity, and maybe a potent sensitizer for laryngeal cancer radiotherapy. [ABSTRACT FROM AUTHOR] |
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| Database: |
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