Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C
| Title: | Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C |
|---|---|
| Authors: | Domingo, Paula, Olaciregui, Maite, González, Noelia, De Blas, Ignacio, Gil, Lydia |
| Source: | Animal Reproduction. October 2019 16(4) |
| Publisher Information: | Colégio Brasileiro de Reprodução Animal, 2019. |
| Publication Year: | 2019 |
| Subject Terms: | cryoprotectant, dimethylformamide, n-methyl-2-pyrrolidone, glycerol, rabbit sperm preservation |
| More Details: | Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl-2-Pyrrolidone (NMP) can improve cooled rabbit sperm quality stored at 4ºC and 16ºC. Sperm samples were diluted with INRA 96® (Extender A), INRA 96® with 6% glycerol (Extender B) or 6% DMF (Extender C) or 6% NMP (Extender D) respectively and stored at 4ºC and 16ºC. Samples were then analysed at 4, 24, 48 and 72 hours after refrigeration by integrated sperm analysis system (ISAS®), eosin-nigrosin stain (vitality), hypo-osmotic swelling test (HOS test) and acrosome integrity test. Extender C showed higher percentage of motility, vitality and HOS test than extender B and D (p<0.05). Whereas sperm quality decreased over time (p<0.05), data showed that the addition of DMF kept the motility and sperm plasma membrane integrity after 24 hours of storage better than other diluents. These results suggest that the addition of DMF to INRA 96® exerts a protective effect on the membrane of spermatozoa improving seminal quality. |
| Document Type: | article |
| File Description: | text/html |
| Language: | English |
| ISSN: | 1984-3143 |
| DOI: | 10.21451/1984-3143-ar2018-0100 |
| Access URL: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432019000400887 |
| Rights: | info:eu-repo/semantics/openAccess |
| Accession Number: | edssci.S1984.31432019000400887 |
| Database: | SciELO |
| FullText | Text: Availability: 0 CustomLinks: – Url: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432019000400887# Name: EDS - SciELO Category: fullText Text: Full Text from SciELO MouseOverText: Full Text from SciELO – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edssci&genre=article&issn=19843143&ISBN=&volume=16&issue=4&date=20191001&spage=&pages=&title=Animal Reproduction&atitle=Effect%20of%20glycerol%2C%20n%2C%20n-dimethylformamide%20and%20n-methyl-2-pyrrolidone%20on%20rabbit%20sperm%20stored%20at%204%20%C2%B0C%20and%2016%20%C2%B0C&aulast=Domingo%2C%20Paula&id=DOI:10.21451/1984-3143-ar2018-0100 Name: Full Text Finder (for New FTF UI) (s8985755) Category: fullText Text: Find It @ SCU Libraries MouseOverText: Find It @ SCU Libraries |
|---|---|
| Header | DbId: edssci DbLabel: SciELO An: edssci.S1984.31432019000400887 RelevancyScore: 934 AccessLevel: 3 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 933.8076171875 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Domingo%2C+Paula%22">Domingo, Paula</searchLink><br /><searchLink fieldCode="AR" term="%22Olaciregui%2C+Maite%22">Olaciregui, Maite</searchLink><br /><searchLink fieldCode="AR" term="%22González%2C+Noelia%22">González, Noelia</searchLink><br /><searchLink fieldCode="AR" term="%22De+Blas%2C+Ignacio%22">De Blas, Ignacio</searchLink><br /><searchLink fieldCode="AR" term="%22Gil%2C+Lydia%22">Gil, Lydia</searchLink> – Name: TitleSource Label: Source Group: Src Data: <i>Animal Reproduction</i>. October 2019 16(4) – Name: Publisher Label: Publisher Information Group: PubInfo Data: Colégio Brasileiro de Reprodução Animal, 2019. – Name: DatePubCY Label: Publication Year Group: Date Data: 2019 – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22cryoprotectant%22">cryoprotectant</searchLink><br /><searchLink fieldCode="DE" term="%22dimethylformamide%22">dimethylformamide</searchLink><br /><searchLink fieldCode="DE" term="%22n-methyl-2-pyrrolidone%22">n-methyl-2-pyrrolidone</searchLink><br /><searchLink fieldCode="DE" term="%22glycerol%22">glycerol</searchLink><br /><searchLink fieldCode="DE" term="%22rabbit+sperm+preservation%22">rabbit sperm preservation</searchLink> – Name: Abstract Label: Description Group: Ab Data: Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl-2-Pyrrolidone (NMP) can improve cooled rabbit sperm quality stored at 4ºC and 16ºC. Sperm samples were diluted with INRA 96® (Extender A), INRA 96® with 6% glycerol (Extender B) or 6% DMF (Extender C) or 6% NMP (Extender D) respectively and stored at 4ºC and 16ºC. Samples were then analysed at 4, 24, 48 and 72 hours after refrigeration by integrated sperm analysis system (ISAS®), eosin-nigrosin stain (vitality), hypo-osmotic swelling test (HOS test) and acrosome integrity test. Extender C showed higher percentage of motility, vitality and HOS test than extender B and D (p<0.05). Whereas sperm quality decreased over time (p<0.05), data showed that the addition of DMF kept the motility and sperm plasma membrane integrity after 24 hours of storage better than other diluents. These results suggest that the addition of DMF to INRA 96® exerts a protective effect on the membrane of spermatozoa improving seminal quality. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article – Name: Format Label: File Description Group: SrcInfo Data: text/html – Name: Language Label: Language Group: Lang Data: English – Name: ISSN Label: ISSN Group: ISSN Data: 1984-3143 – Name: DOI Label: DOI Group: ID Data: 10.21451/1984-3143-ar2018-0100 – Name: URL Label: Access URL Group: URL Data: <link linkTarget="URL" linkTerm="http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432019000400887" linkWindow="_blank">http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432019000400887</link> – Name: Copyright Label: Rights Group: Cpyrght Data: info:eu-repo/semantics/openAccess – Name: AN Label: Accession Number Group: ID Data: edssci.S1984.31432019000400887 |
| PLink | https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edssci&AN=edssci.S1984.31432019000400887 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.21451/1984-3143-ar2018-0100 Languages: – Text: English Subjects: – SubjectFull: cryoprotectant Type: general – SubjectFull: dimethylformamide Type: general – SubjectFull: n-methyl-2-pyrrolidone Type: general – SubjectFull: glycerol Type: general – SubjectFull: rabbit sperm preservation Type: general Titles: – TitleFull: Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Domingo, Paula – PersonEntity: Name: NameFull: Olaciregui, Maite – PersonEntity: Name: NameFull: González, Noelia – PersonEntity: Name: NameFull: De Blas, Ignacio – PersonEntity: Name: NameFull: Gil, Lydia IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 19843143 Numbering: – Type: volume Value: 16 – Type: issue Value: 4 Titles: – TitleFull: Animal Reproduction Type: main |
| ResultId | 1 |