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Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (Diptera: Culicidae)

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Title: Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (Diptera: Culicidae)
Authors: Bello García, Felio J., Boshell, Jorge, Rey, Gloria, Morales, Alberto, Olano, Victor Alberto
Source: Memórias do Instituto Oswaldo Cruz. August 1995 90(4)
Publisher Information: Instituto Oswaldo Cruz, Ministério da Saúde, 1995.
Publication Year: 1995
Subject Terms: mosquitoes, cell cultures, culture media, karyotype
More Details: Primary cell cultures were obtained from eggs of Anopheles albimanus and Aedes taeniorhynchus mosquitoes, vectors of human malaria and of Venezuelan equine encephalitis virus, respectively. The cellular growth of the An. albimanus cells began four weeks after explanting the embryonic tissues in MK/VP12 medium, supplemented with 15% fetal bovine serum. The culture showed heterogeneous cellular morphology. With regard to the Ae. taeniorhynchus culture, growth occurred three weeks after initiating the culture in MM/VP12 medium. The majority of cells were small and round. Karyotypes were examined in the latter species.
Document Type: article
File Description: text/html
Language: English
ISSN: 0074-0276
DOI: 10.1590/S0074-02761995000400024
Access URL: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000400024
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  Data: Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (Diptera: Culicidae)
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  Data: <searchLink fieldCode="AR" term="%22Bello+García%2C+Felio+J%2E%22">Bello García, Felio J.</searchLink><br /><searchLink fieldCode="AR" term="%22Boshell%2C+Jorge%22">Boshell, Jorge</searchLink><br /><searchLink fieldCode="AR" term="%22Rey%2C+Gloria%22">Rey, Gloria</searchLink><br /><searchLink fieldCode="AR" term="%22Morales%2C+Alberto%22">Morales, Alberto</searchLink><br /><searchLink fieldCode="AR" term="%22Olano%2C+Victor+Alberto%22">Olano, Victor Alberto</searchLink>
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  Data: <i>Memórias do Instituto Oswaldo Cruz</i>. August 1995 90(4)
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  Data: Instituto Oswaldo Cruz, Ministério da Saúde, 1995.
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  Data: 1995
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  Data: <searchLink fieldCode="DE" term="%22mosquitoes%22">mosquitoes</searchLink><br /><searchLink fieldCode="DE" term="%22cell+cultures%22">cell cultures</searchLink><br /><searchLink fieldCode="DE" term="%22culture+media%22">culture media</searchLink><br /><searchLink fieldCode="DE" term="%22karyotype%22">karyotype</searchLink>
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  Data: Primary cell cultures were obtained from eggs of Anopheles albimanus and Aedes taeniorhynchus mosquitoes, vectors of human malaria and of Venezuelan equine encephalitis virus, respectively. The cellular growth of the An. albimanus cells began four weeks after explanting the embryonic tissues in MK/VP12 medium, supplemented with 15% fetal bovine serum. The culture showed heterogeneous cellular morphology. With regard to the Ae. taeniorhynchus culture, growth occurred three weeks after initiating the culture in MM/VP12 medium. The majority of cells were small and round. Karyotypes were examined in the latter species.
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              Y: 1995
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