Patent
View in EDS

Fusion proteins forming trimers

Bibliographic Details
Title: Fusion proteins forming trimers
Patent Number: 8,664,366
Publication Date: March 04, 2014
Appl. No: 13/143531
Application Filed: January 09, 2009
Abstract: The present invention refers to fusion proteins comprising a neck region and carbohydrate recognition domain of a collectin trimerization domain, a linker element and an effector polypeptide. Further the invention refers to a nucleic acid encoding the said fusion protein. The fusion proteins, the nucleic acid, and the cell are suitable as pharmaceutical composition or for therapeutic, diagnostic and/or research applications as described herein.
Inventors: Hill, Oliver (Neckarsteinach, DE); Branschaedel, Marcus (Laupheim, DE); Gieffers, Christian (Dossenheim, DE); Thiemann, Meinolf (Schriesheim, DE)
Assignees: Apogenix GmbH (Heidelberg, DE)
Claim: 1. A fusion protein comprising (i) a collectin family trimerization domain consisting of amino acid residues 221-375 of SEQ ID NO: 21, optionally further consisting of an amino acid substitution at residue 355; (ii) a linker element; and (iii) an effector polypeptide selected from the group consisting of a single chain antibody, an antigen binding fragment of a single chain antibody, or an antigen binding fragment of an antibody, wherein the effector polypeptide is located N-terminally of the collectin family neck region wherein said linker element has 25 or less amino acids.
Claim: 2. The fusion protein claim 1 , wherein the collectin family trimerization domain consists of the amino acid residues 221-375 of SEQ ID NO: 21.
Claim: 3. The fusion protein of claim 1 , wherein the amino acid residue 355 in SEQ ID NO: 21 is optionally substituted to a polar amino acid selected from the group consisting of aspartic acid, glutamic acid, asparagine and glutamine.
Claim: 4. The fusion protein of claim 1 , wherein the effector polypeptide is a single chain antibody against IL4R-alpha.
Claim: 5. The fusion protein of claim 1 , wherein the linker element has the amino acid sequence of SEQ ID NO: 56, which is GSSGSSGSSGSG.
Claim: 6. The fusion protein of claim 1 , wherein the linker element has the amino acid sequence of SEQ ID NO: 34, which is GSSGSSGSSGS.
Claim: 7. The fusion protein of claim 1 , further comprising an N-terminal signal peptide domain.
Claim: 8. The fusion protein of claim 1 , further comprising a recognition/purification domain selected from the group consisting of a strep-tag domain and a poly-His domain.
Claim: 9. The fusion protein of claim 1 , wherein the effector polypeptide is a monovalent Fab fragment.
Claim: 10. The fusion protein of claim 1 , wherein the effector polypeptide is a single chain variable fragment (scFv).
Claim: 11. The fusion protein of claim 10 , wherein the scFv has a variable domain of a heavy chain located N-terminal to the a variable domain of a light chain.
Claim: 12. A trimeric complex consisting of three identical fusion proteins of claim 1 .
Current U.S. Class: 5303/873
Patent References Cited: 2004/0047873 March 2004 Al- Shamkhani et al.
2004/0197876 October 2004 Tschopp et al.
2004/0247563 December 2004 Lynch et al.
2009/0325867 December 2009 Cohen et al.
2010/0199364 August 2010 Hill et al.
2010/0322922 December 2010 Martin-Villalba et al.
2011/0111494 May 2011 Hill et al.
WO 95/31540 November 1995
WO 97/01633 January 1997
WO 01/42298 June 2001
WO 02/090553 November 2002
WO 03/086301 October 2003
WO 2004/024925 March 2004
WO 2007/102690 September 2007
WO 2009/007120 January 2009
Other References: Crouch et al., (JBC 2006, 281:18008-18014). cited by examiner
Hakansson, K. and K.B. Reid (2000). “Collectin structure: a review [In Process Citations]”,Protein Science 9: pp. 1607-1617. cited by applicant
Haswell, et al. (2001). “Analysis of the oligomeric requirement for signaling by CD40 using soluble multimeric forms of its ligand, CD154”, Eur. J. Immunol. 31: 3094-3100. cited by applicant
Sano, H. and Y. Kuroki (2005). “The lung colectins, SP-A and SP-D, modulate pulmonary innate immunity”, 42: pp. 279-287. cited by applicant
Kishore, et al. (2006). “Surfactant proteins SP-A and SP-D: Structure, function and receptors”, Molecular Immunology, 43: pp. 1293-1315. cited by applicant
Wu et al.: Molecular Immunology 46:2381-2388, 2009. cited by applicant
Holler, N. et al.: “Two Adjacent Trimeric Fas Ligands Are Required for Fas Signaling and Formation of a Death-Inducing Signaling Complex”, Molecular and Cellular Biology, American Societv for Microbiology. vol. 23 No. 4 Feb. 1, 2003, p. 1428-1440. cited by applicant
Hoppe, H.-J. et al.: “A parallel three stranded a-helical bundle at the nucleation site of collagen triple-helix formation”, FEBS Letters, vol. 344, No. 2/03, Jan. 1, 1994, pp. 191-195. cited by applicant
Kornbluth, R. S. et al.: “CD40L (CD154) fusion protein with pulmonary surfactant protein D as a prototype for soluble multimeric TNF superfamily ligand molecules”, FASEB Journal, Fed. of American Soc. for Experimental Biology. vol. 14 No. 6, Apr. 20, 2000. cited by applicant
Primary Examiner: Stanfield, Cherie M
Attorney, Agent or Firm: Perkins Coie LLP
Kung, Viola T.
Accession Number: edspgr.08664366
Database: USPTO Patent Grants
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://ppubs.uspto.gov/pubwebapp/external.html?q=(%228%2C664%2C366%22).pn.&db=USPAT&type=ids
    Name: EDS - USPTO Patent Grants
    Category: fullText
    Text: View record from USPTO Patent Grants
    MouseOverText: View record from USPTO Patent Grants
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edspgr&genre=article&issn=&ISBN=&volume=&issue=&date=20140304&spage=&pages=&title=Fusion proteins forming trimers&atitle=Fusion%20proteins%20forming%20trimers&aulast=Hill%2C%20Oliver&id=DOI:
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
Header DbId: edspgr
DbLabel: USPTO Patent Grants
An: edspgr.08664366
RelevancyScore: 726
AccessLevel: 3
PubType: Patent
PubTypeId: patent
PreciseRelevancyScore: 726.496337890625
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Fusion proteins forming trimers
– Name: DocumentID
  Label: Patent Number
  Group: Patent
  Data: 8,664,366
– Name: DateEntry
  Label: Publication Date
  Group: Patent
  Data: March 04, 2014
– Name: DocumentID
  Label: Appl. No
  Group: Patent
  Data: 13/143531
– Name: DateFiled
  Label: Application Filed
  Group: Patent
  Data: January 09, 2009
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The present invention refers to fusion proteins comprising a neck region and carbohydrate recognition domain of a collectin trimerization domain, a linker element and an effector polypeptide. Further the invention refers to a nucleic acid encoding the said fusion protein. The fusion proteins, the nucleic acid, and the cell are suitable as pharmaceutical composition or for therapeutic, diagnostic and/or research applications as described herein.
– Name: Author
  Label: Inventors
  Group: Patent
  Data: <searchLink fieldCode="ZA" term="%22Hill%2C+Oliver%22">Hill, Oliver</searchLink> (Neckarsteinach, DE); <searchLink fieldCode="ZA" term="%22Branschaedel%2C+Marcus%22">Branschaedel, Marcus</searchLink> (Laupheim, DE); <searchLink fieldCode="ZA" term="%22Gieffers%2C+Christian%22">Gieffers, Christian</searchLink> (Dossenheim, DE); <searchLink fieldCode="ZA" term="%22Thiemann%2C+Meinolf%22">Thiemann, Meinolf</searchLink> (Schriesheim, DE)
– Name: OtherAuthors
  Label: Assignees
  Group: Patent
  Data: <searchLink fieldCode="ZS" term="%22Apogenix+GmbH%22">Apogenix GmbH</searchLink> (Heidelberg, DE)
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 1. A fusion protein comprising (i) a collectin family trimerization domain consisting of amino acid residues 221-375 of SEQ ID NO: 21, optionally further consisting of an amino acid substitution at residue 355; (ii) a linker element; and (iii) an effector polypeptide selected from the group consisting of a single chain antibody, an antigen binding fragment of a single chain antibody, or an antigen binding fragment of an antibody, wherein the effector polypeptide is located N-terminally of the collectin family neck region wherein said linker element has 25 or less amino acids.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 2. The fusion protein claim 1 , wherein the collectin family trimerization domain consists of the amino acid residues 221-375 of SEQ ID NO: 21.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 3. The fusion protein of claim 1 , wherein the amino acid residue 355 in SEQ ID NO: 21 is optionally substituted to a polar amino acid selected from the group consisting of aspartic acid, glutamic acid, asparagine and glutamine.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 4. The fusion protein of claim 1 , wherein the effector polypeptide is a single chain antibody against IL4R-alpha.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 5. The fusion protein of claim 1 , wherein the linker element has the amino acid sequence of SEQ ID NO: 56, which is GSSGSSGSSGSG.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 6. The fusion protein of claim 1 , wherein the linker element has the amino acid sequence of SEQ ID NO: 34, which is GSSGSSGSSGS.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 7. The fusion protein of claim 1 , further comprising an N-terminal signal peptide domain.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 8. The fusion protein of claim 1 , further comprising a recognition/purification domain selected from the group consisting of a strep-tag domain and a poly-His domain.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 9. The fusion protein of claim 1 , wherein the effector polypeptide is a monovalent Fab fragment.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 10. The fusion protein of claim 1 , wherein the effector polypeptide is a single chain variable fragment (scFv).
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 11. The fusion protein of claim 10 , wherein the scFv has a variable domain of a heavy chain located N-terminal to the a variable domain of a light chain.
– Name: Comment
  Label: Claim
  Group: Patent
  Data: 12. A trimeric complex consisting of three identical fusion proteins of claim 1 .
– Name: CodeClass
  Label: Current U.S. Class
  Group: Patent
  Data: 5303/873
– Name: Ref
  Label: Patent References Cited
  Group: Patent
  Data: <searchLink fieldCode="RF" term="%222004%2E0047873%22">2004/0047873</searchLink> March 2004 Al- Shamkhani et al.<br /><searchLink fieldCode="RF" term="%222004%2E0197876%22">2004/0197876</searchLink> October 2004 Tschopp et al.<br /><searchLink fieldCode="RF" term="%222004%2E0247563%22">2004/0247563</searchLink> December 2004 Lynch et al.<br /><searchLink fieldCode="RF" term="%222009%2E0325867%22">2009/0325867</searchLink> December 2009 Cohen et al.<br /><searchLink fieldCode="RF" term="%222010%2E0199364%22">2010/0199364</searchLink> August 2010 Hill et al.<br /><searchLink fieldCode="RF" term="%222010%2E0322922%22">2010/0322922</searchLink> December 2010 Martin-Villalba et al.<br /><searchLink fieldCode="RF" term="%222011%2E0111494%22">2011/0111494</searchLink> May 2011 Hill et al.<br />WO 95/31540 November 1995<br />WO 97/01633 January 1997<br />WO 01/42298 June 2001<br />WO 02/090553 November 2002<br />WO 03/086301 October 2003<br />WO 2004/024925 March 2004<br />WO 2007/102690 September 2007<br />WO 2009/007120 January 2009<br /><br /><br /><br /><br /><br /><br /><br /><br />
– Name: Ref
  Label: Other References
  Group: Patent
  Data: Crouch et al., (JBC 2006, 281:18008-18014). cited by examiner<br />Hakansson, K. and K.B. Reid (2000). “Collectin structure: a review [In Process Citations]”,Protein Science 9: pp. 1607-1617. cited by applicant<br />Haswell, et al. (2001). “Analysis of the oligomeric requirement for signaling by CD40 using soluble multimeric forms of its ligand, CD154”, Eur. J. Immunol. 31: 3094-3100. cited by applicant<br />Sano, H. and Y. Kuroki (2005). “The lung colectins, SP-A and SP-D, modulate pulmonary innate immunity”, 42: pp. 279-287. cited by applicant<br />Kishore, et al. (2006). “Surfactant proteins SP-A and SP-D: Structure, function and receptors”, Molecular Immunology, 43: pp. 1293-1315. cited by applicant<br />Wu et al.: Molecular Immunology 46:2381-2388, 2009. cited by applicant<br />Holler, N. et al.: “Two Adjacent Trimeric Fas Ligands Are Required for Fas Signaling and Formation of a Death-Inducing Signaling Complex”, Molecular and Cellular Biology, American Societv for Microbiology. vol. 23 No. 4 Feb. 1, 2003, p. 1428-1440. cited by applicant<br />Hoppe, H.-J. et al.: “A parallel three stranded a-helical bundle at the nucleation site of collagen triple-helix formation”, FEBS Letters, vol. 344, No. 2/03, Jan. 1, 1994, pp. 191-195. cited by applicant<br />Kornbluth, R. S. et al.: “CD40L (CD154) fusion protein with pulmonary surfactant protein D as a prototype for soluble multimeric TNF superfamily ligand molecules”, FASEB Journal, Fed. of American Soc. for Experimental Biology. vol. 14 No. 6, Apr. 20, 2000. cited by applicant
– Name: AuthorEditor
  Label: Primary Examiner
  Group: Patent
  Data: <searchLink fieldCode="ZE" term="%22Stanfield%2C+Cherie+M%22">Stanfield, Cherie M</searchLink>
– Name: AuthorCorporate
  Label: Attorney, Agent or Firm
  Group: Patent
  Data: <searchLink fieldCode="ZG" term="%22Perkins+Coie+LLP%22">Perkins Coie LLP</searchLink><br /><searchLink fieldCode="ZG" term="%22Kung%2C+Viola+T%2E%22">Kung, Viola T.</searchLink>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edspgr.08664366
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edspgr&AN=edspgr.08664366
RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Text: English
    Titles:
      – TitleFull: Fusion proteins forming trimers
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Hill, Oliver
      – PersonEntity:
          Name:
            NameFull: Branschaedel, Marcus
      – PersonEntity:
          Name:
            NameFull: Gieffers, Christian
      – PersonEntity:
          Name:
            NameFull: Thiemann, Meinolf
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 04
              M: 03
              Text: March 04, 2014
              Type: published
              Y: 2014
ResultId 1