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A calibrated optogenetic toolbox of stable zebrafish opsin lines

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Title: A calibrated optogenetic toolbox of stable zebrafish opsin lines
Authors: Paride Antinucci, Adna Dumitrescu, Charlotte Deleuze, Holly J Morley, Kristie Leung, Tom Hagley, Fumi Kubo, Herwig Baier, Isaac H Bianco, Claire Wyart
Source: eLife, Vol 9 (2020)
Publisher Information: eLife Sciences Publications Ltd, 2020.
Publication Year: 2020
Collection: LCC:Medicine
LCC:Science
LCC:Biology (General)
Subject Terms: optogenetics, electrophysiology, transgenesis, CoChR, ChrimsonR, GtACR, Medicine, Science, Biology (General), QH301-705.5
More Details: Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2050-084X
Relation: https://elifesciences.org/articles/54937; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.54937
Access URL: https://doaj.org/article/fe82677ca135457dafe67bc4bee7a97f
Accession Number: edsdoj.fe82677ca135457dafe67bc4bee7a97f
Database: Directory of Open Access Journals
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  Data: A calibrated optogenetic toolbox of stable zebrafish opsin lines
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  Data: <searchLink fieldCode="AR" term="%22Paride+Antinucci%22">Paride Antinucci</searchLink><br /><searchLink fieldCode="AR" term="%22Adna+Dumitrescu%22">Adna Dumitrescu</searchLink><br /><searchLink fieldCode="AR" term="%22Charlotte+Deleuze%22">Charlotte Deleuze</searchLink><br /><searchLink fieldCode="AR" term="%22Holly+J+Morley%22">Holly J Morley</searchLink><br /><searchLink fieldCode="AR" term="%22Kristie+Leung%22">Kristie Leung</searchLink><br /><searchLink fieldCode="AR" term="%22Tom+Hagley%22">Tom Hagley</searchLink><br /><searchLink fieldCode="AR" term="%22Fumi+Kubo%22">Fumi Kubo</searchLink><br /><searchLink fieldCode="AR" term="%22Herwig+Baier%22">Herwig Baier</searchLink><br /><searchLink fieldCode="AR" term="%22Isaac+H+Bianco%22">Isaac H Bianco</searchLink><br /><searchLink fieldCode="AR" term="%22Claire+Wyart%22">Claire Wyart</searchLink>
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  Data: eLife, Vol 9 (2020)
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  Data: <searchLink fieldCode="DE" term="%22optogenetics%22">optogenetics</searchLink><br /><searchLink fieldCode="DE" term="%22electrophysiology%22">electrophysiology</searchLink><br /><searchLink fieldCode="DE" term="%22transgenesis%22">transgenesis</searchLink><br /><searchLink fieldCode="DE" term="%22CoChR%22">CoChR</searchLink><br /><searchLink fieldCode="DE" term="%22ChrimsonR%22">ChrimsonR</searchLink><br /><searchLink fieldCode="DE" term="%22GtACR%22">GtACR</searchLink><br /><searchLink fieldCode="DE" term="%22Medicine%22">Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Science%22">Science</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink>
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  Data: Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.
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      – Text: English
    Subjects:
      – SubjectFull: optogenetics
        Type: general
      – SubjectFull: electrophysiology
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      – SubjectFull: transgenesis
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