Enhancing Enzymatic Hydrolysis and Delignification of Sugarcane Bagasse Using Different Concentrations of Sodium Alkaline Pretreatment
| Title: | Enhancing Enzymatic Hydrolysis and Delignification of Sugarcane Bagasse Using Different Concentrations of Sodium Alkaline Pretreatment |
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| Authors: | Arti Yadav, Pushpa Rani, Deepak Kumar Yadav, Nisha Bhardwaj, Asha Gupta and Narsi Ram Bishnoi |
| Source: | Nature Environment and Pollution Technology, Vol 23, Iss 1, Pp 427-434 (2024) |
| Publisher Information: | Technoscience Publications, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Environmental effects of industries and plants LCC:Science (General) |
| Subject Terms: | sugarcane bagasse, enzymatic hydrolysis, pretreatment, delignification, cellic ctec2, Environmental effects of industries and plants, TD194-195, Science (General), Q1-390 |
| More Details: | Lignin, being highly resistant, needs to be eliminated in the process of extraction of soluble reducing sugar and bioethanol production from lignocellulosic biomass. In the present work, pretreatment of sugarcane bagasse (SCB) was performed using NaOH of various concentrations (1-5%) to facilitate delignification. The hydrolysis efficiency of pretreated SCB was evaluated at different reaction times by the production of reducing sugar using the Cellic CTec2 enzyme. The maximum cellulose content of 57.6% and lignin removal of 62.04% were observed with 2% sodium hydroxide at 121°C autoclaved for 60 min. The hemicellulose content decreased with increasing NaOH concentration with the maximum decrease of 13.6% from native bagasse having 26.5% xylan content. The microstructure, morphology, and chemical composition of SCB were analyzed using Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform InfraRed (FTIR), and XRD. The hydrolysis with 10 FPU.g-1 of enzyme at 48 h of reaction time shows a maximum yield of 12.34 g.L-1 corresponding to 55.53 ± 0.45% at 2% NaOH pretreated SCB. This study claims that lignin components exhibited the highest susceptibility to NaOH pretreatment, which directly affects enzymatic hydrolysis. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0972-6268 2395-3454 |
| Relation: | https://neptjournal.com/upload-images/(37)B-4088.pdf; https://doaj.org/toc/0972-6268; https://doaj.org/toc/2395-3454 |
| DOI: | 10.46488/NEPT.2024.v23i01.037 |
| Access URL: | https://doaj.org/article/f67ad79fa426494a9b3f6141a27609f8 |
| Accession Number: | edsdoj.f67ad79fa426494a9b3f6141a27609f8 |
| Database: | Directory of Open Access Journals |
| ISSN: | 09726268 23953454 |
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| DOI: | 10.46488/NEPT.2024.v23i01.037 |
| Published in: | Nature Environment and Pollution Technology |
| Language: | English |