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The Mechanism of Ovule Abortion in Self-Pollinated ‘Hanfu’ Apple Fruits and Related Gene Screening

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Title: The Mechanism of Ovule Abortion in Self-Pollinated ‘Hanfu’ Apple Fruits and Related Gene Screening
Authors: Haiyang Wei, Baoan Wang, Ya Xu, Wenqi Fan, Manyu Zhang, Fuli Huang, Chenxi Shi, Tianzhong Li, Shengnan Wang, Shengyuan Wang
Source: Plants, Vol 13, Iss 7, p 996 (2024)
Publisher Information: MDPI AG, 2024.
Publication Year: 2024
Collection: LCC:Botany
Subject Terms: apple, self-pollination, ovule abortion, SMART-seq, Botany, QK1-989
More Details: Apples exhibit S-RNase-mediated self-incompatibility and typically require cross-pollination in nature. ‘Hanfu’ is a cultivar that produces abundant fruit after self-pollination, although it also shows a high rate of seed abortion afterwards, which greatly reduces fruit quality. In this study, we investigated the ovule development process and the mechanism of ovule abortion in apples after self-pollination. Using a DIC microscope and biomicroscope, we found that the abortion of apple ovules occurs before embryo formation and results from the failure of sperm–egg fusion. Further, we used laser-assisted microdissection (LAM) cutting and sperm and egg cell sequencing at different periods after pollination to obtain the genes related to ovule abortion. The top 40 differentially expressed genes (DEGs) were further verified, and the results were consistent with switching the mechanism at the 5′ end of the RNA transcript (SMART-seq). Through this study, we can preliminarily clarify the mechanism of ovule abortion in self-pollinated apple fruits and provide a gene reserve for further study and improvement of ‘Hanfu’ apple fruit quality.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2223-7747
Relation: https://www.mdpi.com/2223-7747/13/7/996; https://doaj.org/toc/2223-7747
DOI: 10.3390/plants13070996
Access URL: https://doaj.org/article/f5206aeb461549da8b782b9b6bea3afc
Accession Number: edsdoj.f5206aeb461549da8b782b9b6bea3afc
Database: Directory of Open Access Journals
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  Data: The Mechanism of Ovule Abortion in Self-Pollinated ‘Hanfu’ Apple Fruits and Related Gene Screening
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  Data: <searchLink fieldCode="AR" term="%22Haiyang+Wei%22">Haiyang Wei</searchLink><br /><searchLink fieldCode="AR" term="%22Baoan+Wang%22">Baoan Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Ya+Xu%22">Ya Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Wenqi+Fan%22">Wenqi Fan</searchLink><br /><searchLink fieldCode="AR" term="%22Manyu+Zhang%22">Manyu Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Fuli+Huang%22">Fuli Huang</searchLink><br /><searchLink fieldCode="AR" term="%22Chenxi+Shi%22">Chenxi Shi</searchLink><br /><searchLink fieldCode="AR" term="%22Tianzhong+Li%22">Tianzhong Li</searchLink><br /><searchLink fieldCode="AR" term="%22Shengnan+Wang%22">Shengnan Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Shengyuan+Wang%22">Shengyuan Wang</searchLink>
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  Data: Plants, Vol 13, Iss 7, p 996 (2024)
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  Data: MDPI AG, 2024.
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  Data: <searchLink fieldCode="DE" term="%22apple%22">apple</searchLink><br /><searchLink fieldCode="DE" term="%22self-pollination%22">self-pollination</searchLink><br /><searchLink fieldCode="DE" term="%22ovule+abortion%22">ovule abortion</searchLink><br /><searchLink fieldCode="DE" term="%22SMART-seq%22">SMART-seq</searchLink><br /><searchLink fieldCode="DE" term="%22Botany%22">Botany</searchLink><br /><searchLink fieldCode="DE" term="%22QK1-989%22">QK1-989</searchLink>
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  Data: Apples exhibit S-RNase-mediated self-incompatibility and typically require cross-pollination in nature. ‘Hanfu’ is a cultivar that produces abundant fruit after self-pollination, although it also shows a high rate of seed abortion afterwards, which greatly reduces fruit quality. In this study, we investigated the ovule development process and the mechanism of ovule abortion in apples after self-pollination. Using a DIC microscope and biomicroscope, we found that the abortion of apple ovules occurs before embryo formation and results from the failure of sperm–egg fusion. Further, we used laser-assisted microdissection (LAM) cutting and sperm and egg cell sequencing at different periods after pollination to obtain the genes related to ovule abortion. The top 40 differentially expressed genes (DEGs) were further verified, and the results were consistent with switching the mechanism at the 5′ end of the RNA transcript (SMART-seq). Through this study, we can preliminarily clarify the mechanism of ovule abortion in self-pollinated apple fruits and provide a gene reserve for further study and improvement of ‘Hanfu’ apple fruit quality.
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        StartPage: 996
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        Type: general
      – SubjectFull: self-pollination
        Type: general
      – SubjectFull: ovule abortion
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