RNA m6A methylation participates in regulation of postnatal development of the mouse cerebellum
| Title: | RNA m6A methylation participates in regulation of postnatal development of the mouse cerebellum |
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| Authors: | Chunhui Ma, Mengqi Chang, Hongyi Lv, Zhi-Wei Zhang, Weilong Zhang, Xue He, Gaolang Wu, Shunli Zhao, Yao Zhang, Di Wang, Xufei Teng, Chunying Liu, Qing Li, Arne Klungland, Yamei Niu, Shuhui Song, Wei-Min Tong |
| Source: | Genome Biology, Vol 19, Iss 1, Pp 1-18 (2018) |
| Publisher Information: | BMC, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Biology (General) LCC:Genetics |
| Subject Terms: | N6-methyladenosine, RNA methylation, ALKBH5, METTL3, Cerebellar development, Biology (General), QH301-705.5, Genetics, QH426-470 |
| More Details: | Abstract Background N6-methyladenosine (m6A) is an important epitranscriptomic mark with high abundance in the brain. Recently, it has been found to be involved in the regulation of memory formation and mammalian cortical neurogenesis. However, while it is now established that m6A methylation occurs in a spatially restricted manner, its functions in specific brain regions still await elucidation. Results We identify widespread and dynamic RNA m6A methylation in the developing mouse cerebellum and further uncover distinct features of continuous and temporal-specific m6A methylation across the four postnatal developmental processes. Temporal-specific m6A peaks from P7 to P60 exhibit remarkable changes in their distribution patterns along the mRNA transcripts. We also show spatiotemporal-specific expression of m6A writers METTL3, METTL14, and WTAP and erasers ALKBH5 and FTO in the mouse cerebellum. Ectopic expression of METTL3 mediated by lentivirus infection leads to disorganized structure of both Purkinje and glial cells. In addition, under hypobaric hypoxia exposure, Alkbh5-deletion causes abnormal cell proliferation and differentiation in the cerebellum through disturbing the balance of RNA m6A methylation in different cell fate determination genes. Notably, nuclear export of the hypermethylated RNAs is enhanced in the cerebellum of Alkbh5-deficient mice exposed to hypobaric hypoxia. Conclusions Together, our findings provide strong evidence that RNA m6A methylation is controlled in a precise spatiotemporal manner and participates in the regulation of postnatal development of the mouse cerebellum. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1474-760X |
| Relation: | http://link.springer.com/article/10.1186/s13059-018-1435-z; https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-018-1435-z |
| Access URL: | https://doaj.org/article/beadf29692594d6c96ef7d2d1cbdb7ba |
| Accession Number: | edsdoj.beadf29692594d6c96ef7d2d1cbdb7ba |
| Database: | Directory of Open Access Journals |
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