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Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis

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Title: Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis
Authors: Xiao Du, Hongxiu Li, Shiyi Shen, Chao Tian, Xiaohuan Cao, Xingang Xu, Nan Xu, Shuling Wang, Qingchang Tian
Source: Frontiers in Molecular Biosciences, Vol 12 (2025)
Publisher Information: Frontiers Media S.A., 2025.
Publication Year: 2025
Collection: LCC:Biology (General)
Subject Terms: extracellular vesicles, antibody-DNA conjugates, Poisson distribution, proximity ligation technology, tumor diagnosis, Biology (General), QH301-705.5
More Details: IntroductionExtracellular vesicles (EVs) shed from tumor cells into peripheral circulation or other body fluids are promising biomarkers for cancer diagnosis with enormously long circulation. Consequently, precise methods for differentiating normal and tumor-associated EVs (TAEs) are required.MethodsThis study used quantifiable antibody-DNA conjugate-assisted quantitative methods combined with proximity ligation technology to detect TAEs. The antibody-DNA conjugate contained one antibody associated with three oligonucleotides for signal amplification. The antibody in the conjugate can recognize the surface tumor antigens of TAEs. Simultaneously, DNA in the conjugate is attached to the surfaces of TAEs and holds the signal amplification post, converting protein identities to DNA amplification for protein detection, even at the molecular level.ResultsThese findings revealed that TAEs can be quantitatively detected using DNA-mediated quantitative polymerase chain reaction (qPCR). Antibody-DNA conjugates were used to recognize the epithelial cell adhesion molecule (EpCAM) antigen on the TAE surface and quantify the antigen using qPCR for cancer analysis.DiscussionThis method proposed a new quantitative detection approach for TAEs, which aim to identify specific EV-associated markers for diagnostic or therapeutic, this method could inspire a new idea for tumor diagnosis and detection of other diseases.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2296-889X
Relation: https://www.frontiersin.org/articles/10.3389/fmolb.2025.1531108/full; https://doaj.org/toc/2296-889X
DOI: 10.3389/fmolb.2025.1531108
Access URL: https://doaj.org/article/b0253a1cf44a49b5ac7b47278bdd45a0
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  Data: <searchLink fieldCode="AR" term="%22Xiao+Du%22">Xiao Du</searchLink><br /><searchLink fieldCode="AR" term="%22Hongxiu+Li%22">Hongxiu Li</searchLink><br /><searchLink fieldCode="AR" term="%22Shiyi+Shen%22">Shiyi Shen</searchLink><br /><searchLink fieldCode="AR" term="%22Chao+Tian%22">Chao Tian</searchLink><br /><searchLink fieldCode="AR" term="%22Xiaohuan+Cao%22">Xiaohuan Cao</searchLink><br /><searchLink fieldCode="AR" term="%22Xingang+Xu%22">Xingang Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Nan+Xu%22">Nan Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Shuling+Wang%22">Shuling Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Qingchang+Tian%22">Qingchang Tian</searchLink>
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  Data: Frontiers in Molecular Biosciences, Vol 12 (2025)
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  Data: <searchLink fieldCode="DE" term="%22extracellular+vesicles%22">extracellular vesicles</searchLink><br /><searchLink fieldCode="DE" term="%22antibody-DNA+conjugates%22">antibody-DNA conjugates</searchLink><br /><searchLink fieldCode="DE" term="%22Poisson+distribution%22">Poisson distribution</searchLink><br /><searchLink fieldCode="DE" term="%22proximity+ligation+technology%22">proximity ligation technology</searchLink><br /><searchLink fieldCode="DE" term="%22tumor+diagnosis%22">tumor diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink>
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  Data: IntroductionExtracellular vesicles (EVs) shed from tumor cells into peripheral circulation or other body fluids are promising biomarkers for cancer diagnosis with enormously long circulation. Consequently, precise methods for differentiating normal and tumor-associated EVs (TAEs) are required.MethodsThis study used quantifiable antibody-DNA conjugate-assisted quantitative methods combined with proximity ligation technology to detect TAEs. The antibody-DNA conjugate contained one antibody associated with three oligonucleotides for signal amplification. The antibody in the conjugate can recognize the surface tumor antigens of TAEs. Simultaneously, DNA in the conjugate is attached to the surfaces of TAEs and holds the signal amplification post, converting protein identities to DNA amplification for protein detection, even at the molecular level.ResultsThese findings revealed that TAEs can be quantitatively detected using DNA-mediated quantitative polymerase chain reaction (qPCR). Antibody-DNA conjugates were used to recognize the epithelial cell adhesion molecule (EpCAM) antigen on the TAE surface and quantify the antigen using qPCR for cancer analysis.DiscussionThis method proposed a new quantitative detection approach for TAEs, which aim to identify specific EV-associated markers for diagnostic or therapeutic, this method could inspire a new idea for tumor diagnosis and detection of other diseases.
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        Value: 10.3389/fmolb.2025.1531108
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      – Text: English
    Subjects:
      – SubjectFull: extracellular vesicles
        Type: general
      – SubjectFull: antibody-DNA conjugates
        Type: general
      – SubjectFull: Poisson distribution
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