Epidemiology and immunodiagnostics of Strongyloides stercoralis infections among migrant workers in Malaysia
| Title: | Epidemiology and immunodiagnostics of Strongyloides stercoralis infections among migrant workers in Malaysia |
|---|---|
| Authors: | Norhidayu Sahimin, Yvonne A.L. Lim, Rahmah Noordin, Muhammad Hafiznur Yunus, Norsyahida Arifin, Jerzy Marian Behnke, Siti Nursheena Mohd Zain |
| Source: | Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 6, Pp 250-257 (2019) |
| Publisher Information: | Wolters Kluwer Medknow Publications, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Arctic medicine. Tropical medicine |
| Subject Terms: | strongyloides stercoralis, migrant workers, microscopic examination, serological analysis, pcr, Arctic medicine. Tropical medicine, RC955-962 |
| More Details: | Objective: To investigate the status of Strongyloides(S.) stercoralis infections among migrant workers in Malaysia for the first time and identify risk factors. Methods: Four diagnostic methods were employed for the detection of S. stercoralis including microscopy, enzyme-linked immunosorbent assay (ELISA) using a commercial kit, ELISA using the rSs1a antigen and polymerase chain reaction (PCR). Low and semi-skilled workers from five working sectors (i.e. manufacturing, food service, agriculture and plantation, construction and domestic service) were tested on a voluntary basis. Results: The overall seroprevalence of S. stercoralis from 483 workers employing the ELISA commercial kit for IgG was 35.8% (n=173; 95% CI: 31.5%-40.1%) whereas seroprevalence using the rSs1a-ELISA was 13.0% (n=63; 95% CI: 10.0%-16.0%). Cross tabulation between the ELISA commercial kit and rSs1a-ELISA showed that only 6.4% (n=31; 95% CI: 4.2%-8.6%) of the samples were positive in both tests. Microscopic examination of all 388 fecal samples were negative; however subsequent testing by a nested PCR against DNA from the same samples successfully amplified DNA from three male subjects (0.8%; 3/388). Male workers, India and Myanmar nationality, food service occupation and those living in the hostel were statistically significant with seroprevalence (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2352-4146 1995-7645 |
| Relation: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=6;spage=250;epage=257;aulast=Sahimin; https://doaj.org/toc/2352-4146 |
| DOI: | 10.4103/1995-7645.261271 |
| Access URL: | https://doaj.org/article/dac9a28621644122b3a512c458007177 |
| Accession Number: | edsdoj.9a28621644122b3a512c458007177 |
| Database: | Directory of Open Access Journals |
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