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Global molecular analyses of methane metabolism in methanotrophic alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: transcriptomic study

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Title: Global molecular analyses of methane metabolism in methanotrophic alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: transcriptomic study
Authors: Janet B Matsen, Song eYang, Lisa Y Stein, David A. C. Beck, Marina G. Kalyuzhanaya
Source: Frontiers in Microbiology, Vol 4 (2013)
Publisher Information: Frontiers Media S.A., 2013.
Publication Year: 2013
Collection: LCC:Microbiology
Subject Terms: methane metabolism, alphaproteobacterial methanotrophs, Methylosinus trichosporium OB3b, Serine cycle, EMC pathway, Microbiology, QR1-502
More Details: Methane-utilizing bacteria (methanotrophs) are important in both environmental and biotechnological applications, due to their ability to convert methane to multicarbon compounds. However, systems-level studies of methane metabolism have not been carried out in methanotrophs. In this work we have integrated genomic and transcriptomic information to provide an overview of central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotroph. Particulate methane monooxygenase, PQQ-dependent methanol dehydrogenase, the H4MPT-pathway and NAD-dependent formate dehydrogenase are involved in methane oxidation to CO2. All genes essential for operation of the serine cycle, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle were expressed. PEP-pyruvate-oxaloacetate interconversions may have a function in regulation and balancing carbon between the serine cycle and the EMC pathway. A set of transaminases may contribute to carbon partitioning between the pathways. Metabolic pathways for acquisition and/or assimilation of nitrogen and iron are discussed.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1664-302X
Relation: http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00040/full; https://doaj.org/toc/1664-302X
DOI: 10.3389/fmicb.2013.00040
Access URL: https://doaj.org/article/ec98b8d55b1b493abcc36c8c53f51a58
Accession Number: edsdoj.98b8d55b1b493abcc36c8c53f51a58
Database: Directory of Open Access Journals
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  Data: Global molecular analyses of methane metabolism in methanotrophic alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: transcriptomic study
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  Data: <searchLink fieldCode="AR" term="%22Janet+B+Matsen%22">Janet B Matsen</searchLink><br /><searchLink fieldCode="AR" term="%22Song+eYang%22">Song eYang</searchLink><br /><searchLink fieldCode="AR" term="%22Lisa+Y+Stein%22">Lisa Y Stein</searchLink><br /><searchLink fieldCode="AR" term="%22David+A%2E+C%2E+Beck%22">David A. C. Beck</searchLink><br /><searchLink fieldCode="AR" term="%22Marina+G%2E+Kalyuzhanaya%22">Marina G. Kalyuzhanaya</searchLink>
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  Data: Frontiers in Microbiology, Vol 4 (2013)
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  Data: <searchLink fieldCode="DE" term="%22methane+metabolism%22">methane metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22alphaproteobacterial+methanotrophs%22">alphaproteobacterial methanotrophs</searchLink><br /><searchLink fieldCode="DE" term="%22Methylosinus+trichosporium+OB3b%22">Methylosinus trichosporium OB3b</searchLink><br /><searchLink fieldCode="DE" term="%22Serine+cycle%22">Serine cycle</searchLink><br /><searchLink fieldCode="DE" term="%22EMC+pathway%22">EMC pathway</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22QR1-502%22">QR1-502</searchLink>
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  Data: Methane-utilizing bacteria (methanotrophs) are important in both environmental and biotechnological applications, due to their ability to convert methane to multicarbon compounds. However, systems-level studies of methane metabolism have not been carried out in methanotrophs. In this work we have integrated genomic and transcriptomic information to provide an overview of central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotroph. Particulate methane monooxygenase, PQQ-dependent methanol dehydrogenase, the H4MPT-pathway and NAD-dependent formate dehydrogenase are involved in methane oxidation to CO2. All genes essential for operation of the serine cycle, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle were expressed. PEP-pyruvate-oxaloacetate interconversions may have a function in regulation and balancing carbon between the serine cycle and the EMC pathway. A set of transaminases may contribute to carbon partitioning between the pathways. Metabolic pathways for acquisition and/or assimilation of nitrogen and iron are discussed.
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      – SubjectFull: methane metabolism
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      – SubjectFull: alphaproteobacterial methanotrophs
        Type: general
      – SubjectFull: Methylosinus trichosporium OB3b
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      – TitleFull: Global molecular analyses of methane metabolism in methanotrophic alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: transcriptomic study
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