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Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

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Title: Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro
Authors: Wu Changjun, Dai Chenwei, Tong Lin, Lv Han, Zhou Xiuhong
Source: Polish Journal of Microbiology, Vol 71, Iss 1, Pp 91-105 (2022)
Publisher Information: Sciendo, 2022.
Publication Year: 2022
Collection: LCC:Genetics
LCC:Microbiology
Subject Terms: fermented buffalo milk, lactic acid bacteria, probiotic potential, lactobacillus delbrueckii ssp. indicus, food application, Genetics, QH426-470, Microbiology, QR1-502
More Details: The present study aimed to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from Chinese traditional fermented buffalo milk. Out of 22 isolates, 11 were putatively identified as LAB preliminarily. A total of six LAB strains displayed strong adhesion to HT-29 cells and all these strains showed preferable tolerance to artificially simulated gastrointestinal juices. WDS-4, WDS-7, and WDS-18 exhibited excellent antioxidant capacities, including DPPH radical, ABTS+ radical, and superoxide anion scavenging activities. Compared with the other two LAB strains, WDS-7 had a stronger inhibition effect on four pathogens. Based on the 16S rRNA gene sequencing and phylogenetic analysis, WDS-7 was identified as Lactobacillus delbrueckii ssp. indicus and selected to assess the potential and safety of probiotics further. The results revealed that WDS-7 strain had a strong capacity for acid production and good thermal stability. WDS-7 strain also possessed bile salt hydrolase (BSH) activity. Compared to LGG, WDS-7 was a greater biofilm producer on the plastic surface and exhibited a better EPS production ability (1.94 mg/ml as a glucose equivalent). WDS-7 was proved to be sensitive in the majority of tested antibiotics and absence of hemolytic activity. Moreover, no production of biogenic amines and β-glucuronidase was observed in WDS-7. The findings of this work indicated that L. delbrueckii ssp. indicus WDS-7 fulfilled the probiotic criteria in vitro and could be exploited for further evaluation in vivo.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2544-4646
Relation: https://doaj.org/toc/2544-4646
DOI: 10.33073/pjm-2022-012
Access URL: https://doaj.org/article/a948f30134594538aad77b092bc07102
Accession Number: edsdoj.948f30134594538aad77b092bc07102
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  Data: Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro
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  Data: <searchLink fieldCode="AR" term="%22Wu+Changjun%22">Wu Changjun</searchLink><br /><searchLink fieldCode="AR" term="%22Dai+Chenwei%22">Dai Chenwei</searchLink><br /><searchLink fieldCode="AR" term="%22Tong+Lin%22">Tong Lin</searchLink><br /><searchLink fieldCode="AR" term="%22Lv+Han%22">Lv Han</searchLink><br /><searchLink fieldCode="AR" term="%22Zhou+Xiuhong%22">Zhou Xiuhong</searchLink>
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  Data: Polish Journal of Microbiology, Vol 71, Iss 1, Pp 91-105 (2022)
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  Data: LCC:Genetics<br />LCC:Microbiology
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  Data: <searchLink fieldCode="DE" term="%22fermented+buffalo+milk%22">fermented buffalo milk</searchLink><br /><searchLink fieldCode="DE" term="%22lactic+acid+bacteria%22">lactic acid bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22probiotic+potential%22">probiotic potential</searchLink><br /><searchLink fieldCode="DE" term="%22lactobacillus+delbrueckii+ssp%2E+indicus%22">lactobacillus delbrueckii ssp. indicus</searchLink><br /><searchLink fieldCode="DE" term="%22food+application%22">food application</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22QH426-470%22">QH426-470</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22QR1-502%22">QR1-502</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: The present study aimed to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from Chinese traditional fermented buffalo milk. Out of 22 isolates, 11 were putatively identified as LAB preliminarily. A total of six LAB strains displayed strong adhesion to HT-29 cells and all these strains showed preferable tolerance to artificially simulated gastrointestinal juices. WDS-4, WDS-7, and WDS-18 exhibited excellent antioxidant capacities, including DPPH radical, ABTS+ radical, and superoxide anion scavenging activities. Compared with the other two LAB strains, WDS-7 had a stronger inhibition effect on four pathogens. Based on the 16S rRNA gene sequencing and phylogenetic analysis, WDS-7 was identified as Lactobacillus delbrueckii ssp. indicus and selected to assess the potential and safety of probiotics further. The results revealed that WDS-7 strain had a strong capacity for acid production and good thermal stability. WDS-7 strain also possessed bile salt hydrolase (BSH) activity. Compared to LGG, WDS-7 was a greater biofilm producer on the plastic surface and exhibited a better EPS production ability (1.94 mg/ml as a glucose equivalent). WDS-7 was proved to be sensitive in the majority of tested antibiotics and absence of hemolytic activity. Moreover, no production of biogenic amines and β-glucuronidase was observed in WDS-7. The findings of this work indicated that L. delbrueckii ssp. indicus WDS-7 fulfilled the probiotic criteria in vitro and could be exploited for further evaluation in vivo.
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