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Establishment of Tissue Culture System of Actinidia deliciosa Cultivar 'Guichang'

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Title: Establishment of Tissue Culture System of Actinidia deliciosa Cultivar 'Guichang'
Authors: Weimin Zhong, Junliang Zhou, Dongmei Tang, Yaxin Huang, Futao Liu, Min Zhang, Guoli Wang, Sufang Wu, Yue He, Jingwen Tang
Source: Journal of Chemistry, Vol 2021 (2021)
Publisher Information: Wiley, 2021.
Publication Year: 2021
Collection: LCC:Chemistry
Subject Terms: Chemistry, QD1-999
More Details: In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2090-9071
Relation: https://doaj.org/toc/2090-9071
DOI: 10.1155/2021/9951949
Access URL: https://doaj.org/article/d8ecc2dc20c14ebdaf027ca15b53f401
Accession Number: edsdoj.8ecc2dc20c14ebdaf027ca15b53f401
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  Data: Establishment of Tissue Culture System of Actinidia deliciosa Cultivar 'Guichang'
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  Data: <searchLink fieldCode="AR" term="%22Weimin+Zhong%22">Weimin Zhong</searchLink><br /><searchLink fieldCode="AR" term="%22Junliang+Zhou%22">Junliang Zhou</searchLink><br /><searchLink fieldCode="AR" term="%22Dongmei+Tang%22">Dongmei Tang</searchLink><br /><searchLink fieldCode="AR" term="%22Yaxin+Huang%22">Yaxin Huang</searchLink><br /><searchLink fieldCode="AR" term="%22Futao+Liu%22">Futao Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Min+Zhang%22">Min Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Guoli+Wang%22">Guoli Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Sufang+Wu%22">Sufang Wu</searchLink><br /><searchLink fieldCode="AR" term="%22Yue+He%22">Yue He</searchLink><br /><searchLink fieldCode="AR" term="%22Jingwen+Tang%22">Jingwen Tang</searchLink>
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  Data: Journal of Chemistry, Vol 2021 (2021)
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– Name: Abstract
  Label: Description
  Group: Ab
  Data: In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets.
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        Value: 10.1155/2021/9951949
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      – SubjectFull: QD1-999
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