Academic Journal
[Retracted] CuO-TiO2-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line
| Title: | [Retracted] CuO-TiO2-Chitosan-Berbamine Nanocomposites Induce Apoptosis through the Mitochondrial Pathway with the Expression of P53, BAX, and BCL-2 in the Human K562 Cancer Cell Line |
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| Authors: | Abozer Y. Elderdery, Badr Alzahrani, Siddiqa M. A Hamza, Gomaa Mostafa-Hedeab, Pooi Ling Mok, Suresh Kumar Subbiah |
| Source: | Bioinorganic Chemistry and Applications, Vol 2022 (2022) |
| Publisher Information: | Wiley, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Biotechnology LCC:Inorganic chemistry |
| Subject Terms: | Biotechnology, TP248.13-248.65, Inorganic chemistry, QD146-197 |
| More Details: | In this study, cells from human Chronic Myelogenous Leukemia (K562) were cultivated with CuO-TiO2-Chitosan-Berbamine nanocomposites. We examined nanocomposites using XRD, DLS, FESEM, TEM, PL, EDAX, and FTIR spectroscopy, as well as MTT for cytotoxicity, and AO/EtBr for apoptotic morphology assessment. The rate of apoptosis and cell cycle arrests was determined using flow cytometry. Flow cytometry was also employed to identify pro- and antiapoptotic proteins such as Bcl2, Bad, Bax, P53, and Cyt C. The FTIR spectrum revealed that the CuO-TiO2-Chitosan-Berbamine nanocomposites were electrostatically interlocked. The nanocomposites’ XRD signals revealed a hexagonal shape. In the DLS spectrum, nanocomposites were found to have a hydrodynamic diameter. As a result of their cytotoxic action, nanocomposites displayed concentration-dependent cytotoxicity. The nanocomposites, like Doxorubicin, caused cell cycle phase arrest in K562 cells. After treatment with IC50 concentrations of CuO-TiO2-Chitosan-Berbamine nanocomposites and Doxorubicin, a substantial percentage of cells were in G2/M stage arrest. Caspase-3, -7, -8, -9, Bax, Bad, Cyt C, and P53 expression were considerably enhanced in K562 cells, whereas Bcl2 expression was decreased, indicating that these cells may have therapeutic potential against human blood cancer/leukemia-derived disorders. As a result, the nanocomposites demonstrated outstanding anticancer potential against leukemic cells. CuO-TiO2-Chitosan-Berbamine, according to our findings. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1687-479X |
| Relation: | https://doaj.org/toc/1687-479X |
| DOI: | 10.1155/2022/9602725 |
| Access URL: | https://doaj.org/article/85bf905ef52a48b5847d3ca672f11523 |
| Accession Number: | edsdoj.85bf905ef52a48b5847d3ca672f11523 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 1687479X |
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| DOI: | 10.1155/2022/9602725 |
| Published in: | Bioinorganic Chemistry and Applications |
| Language: | English |