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Integrating GC-MS and comparative transcriptome analysis reveals that TsERF66 promotes the biosynthesis of caryophyllene in Toona sinensis tender leaves

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Title: Integrating GC-MS and comparative transcriptome analysis reveals that TsERF66 promotes the biosynthesis of caryophyllene in Toona sinensis tender leaves
Authors: Jianhua Dai, Minyan Wang, Hengfu Yin, Xiaojiao Han, Yanru Fan, Yi Wei, Jie Lin, Jun Liu
Source: Frontiers in Plant Science, Vol 15 (2024)
Publisher Information: Frontiers Media S.A., 2024.
Publication Year: 2024
Collection: LCC:Plant culture
Subject Terms: Toona sinensis, caryophyllene, AP2/ERF, Terpene synthase (TPS), transient overexpression, Plant culture, SB1-1110
More Details: IntroductionThe strong aromatic characteristics of the tender leaves of Toona sinensis determine their quality and economic value.Methods and resultsHere, GC-MS analysis revealed that caryophyllene is a key volatile compound in the tender leaves of two different T. sinensis varieties, however, the transcriptional mechanisms controlling its gene expression are unknown. Comparative transcriptome analysis revealed significant enrichment of terpenoid synthesis pathway genes, suggesting that the regulation of terpenoid synthesis-related gene expression is an important factor leading to differences in aroma between the two varieties. Further analysis of expression levels and genetic evolution revealed that TsTPS18 is a caryophyllene synthase, which was confirmed by transient overexpression in T. sinensis and Nicotiana benthamiana leaves. Furthermore, we screened an AP2/ERF transcriptional factor ERF-IX member, TsERF66, for the potential regulation of caryophyllene synthesis. The TsERF66 had a similar expression trend to that of TsTPS18 and was highly expressed in high-aroma varieties and tender leaves. Exogenous spraying of MeJA also induced the expression of TsERF66 and TsTPS18 and promoted the biosynthesis of caryophyllene. Transient overexpression of TsERF66 in T. sinensis significantly promoted TsTPS18 expression and caryophyllene biosynthesis.DiscussionOur results showed that TsERF66 promoted the expression of TsTPS18 and the biosynthesis of caryophyllene in T. sinensis leaves, providing a strategy for improving the aroma of tender leaves.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1664-462X
Relation: https://www.frontiersin.org/articles/10.3389/fpls.2024.1378418/full; https://doaj.org/toc/1664-462X
DOI: 10.3389/fpls.2024.1378418
Access URL: https://doaj.org/article/ca70324e1bcd4c149dee3ccc86cf4a9c
Accession Number: edsdoj.70324e1bcd4c149dee3ccc86cf4a9c
Database: Directory of Open Access Journals
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  Data: Integrating GC-MS and comparative transcriptome analysis reveals that TsERF66 promotes the biosynthesis of caryophyllene in Toona sinensis tender leaves
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  Data: <searchLink fieldCode="AR" term="%22Jianhua+Dai%22">Jianhua Dai</searchLink><br /><searchLink fieldCode="AR" term="%22Minyan+Wang%22">Minyan Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Hengfu+Yin%22">Hengfu Yin</searchLink><br /><searchLink fieldCode="AR" term="%22Xiaojiao+Han%22">Xiaojiao Han</searchLink><br /><searchLink fieldCode="AR" term="%22Yanru+Fan%22">Yanru Fan</searchLink><br /><searchLink fieldCode="AR" term="%22Yi+Wei%22">Yi Wei</searchLink><br /><searchLink fieldCode="AR" term="%22Jie+Lin%22">Jie Lin</searchLink><br /><searchLink fieldCode="AR" term="%22Jun+Liu%22">Jun Liu</searchLink>
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  Data: Frontiers in Plant Science, Vol 15 (2024)
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  Data: <searchLink fieldCode="DE" term="%22Toona+sinensis%22">Toona sinensis</searchLink><br /><searchLink fieldCode="DE" term="%22caryophyllene%22">caryophyllene</searchLink><br /><searchLink fieldCode="DE" term="%22AP2%2FERF%22">AP2/ERF</searchLink><br /><searchLink fieldCode="DE" term="%22Terpene+synthase+%28TPS%29%22">Terpene synthase (TPS)</searchLink><br /><searchLink fieldCode="DE" term="%22transient+overexpression%22">transient overexpression</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+culture%22">Plant culture</searchLink><br /><searchLink fieldCode="DE" term="%22SB1-1110%22">SB1-1110</searchLink>
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  Label: Description
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  Data: IntroductionThe strong aromatic characteristics of the tender leaves of Toona sinensis determine their quality and economic value.Methods and resultsHere, GC-MS analysis revealed that caryophyllene is a key volatile compound in the tender leaves of two different T. sinensis varieties, however, the transcriptional mechanisms controlling its gene expression are unknown. Comparative transcriptome analysis revealed significant enrichment of terpenoid synthesis pathway genes, suggesting that the regulation of terpenoid synthesis-related gene expression is an important factor leading to differences in aroma between the two varieties. Further analysis of expression levels and genetic evolution revealed that TsTPS18 is a caryophyllene synthase, which was confirmed by transient overexpression in T. sinensis and Nicotiana benthamiana leaves. Furthermore, we screened an AP2/ERF transcriptional factor ERF-IX member, TsERF66, for the potential regulation of caryophyllene synthesis. The TsERF66 had a similar expression trend to that of TsTPS18 and was highly expressed in high-aroma varieties and tender leaves. Exogenous spraying of MeJA also induced the expression of TsERF66 and TsTPS18 and promoted the biosynthesis of caryophyllene. Transient overexpression of TsERF66 in T. sinensis significantly promoted TsTPS18 expression and caryophyllene biosynthesis.DiscussionOur results showed that TsERF66 promoted the expression of TsTPS18 and the biosynthesis of caryophyllene in T. sinensis leaves, providing a strategy for improving the aroma of tender leaves.
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