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Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by n-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene

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Title: Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by n-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene
Authors: Shuai Huang, Ying Bi, Hui Li, Caihong Liu, Xue Wang, Xinyu Wang, Yaxin Lei, Qi Zhang, Jing Wang
Source: Foods, Vol 12, Iss 9, p 1904 (2023)
Publisher Information: MDPI AG, 2023.
Publication Year: 2023
Collection: LCC:Chemical technology
Subject Terms: n-butanol, Hami melon, chilling injury, membrane lipid metabolism, gene cloning, Chemical technology, TP1-1185
More Details: To investigate the effect of n-butanol on postharvest membrane lipid metabolism of Hami melon (Cucumis melo ‘Hami’), the fruits were soaked in a 1.0% solution of n-butanol for 30 min with water as the control. Symptoms of chilling injury were observed regularly, and the indices related to permeability and membrane lipid metabolism of pericarp cells were measured. The results showed that treatment with n-butanol inhibited the increase in chilling injury index, membrane permeability, and malondialdehyde content of Hami melon fruits, promoted an increase in the contents of phosphatidyl alcohol and unsaturated fatty acids, such as linoleic acid, linolenic acid, oleic acid (except 14 d), and erucic acid (28–42 d), and decreased the content of saturated fatty acids, stearic acid (0–28 d), phosphatidic acid (except for 21 d), and the key enzymes of membrane lipid metabolism compared with the control. The activities of phospholipase D (PLD) and lipoxygenase (LOX) and the downregulation of the levels of expression CmPLD-β and CmLOX (42 d only) genes reduced the chilling injury index of Hami melon and alleviated the further expansion of chilling injury symptoms in the fruits. We also cloned the key gene of membrane lipid metabolism CmPLD-β, which was obtained by pre-transcriptome screening of the pericarp. We found that CmPLD-β of Hami melon had the closest affinity with cucumber (CsXP5), indicating that the CmPLD-β gene of Hami melon was functionally similar to that of cucumber. In addition, a two-fold alignment analysis of CmPLD-β and CmXP5 base sequences indicated that the base sequences of the two promoter regions differed from each other.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2304-8158
Relation: https://www.mdpi.com/2304-8158/12/9/1904; https://doaj.org/toc/2304-8158
DOI: 10.3390/foods12091904
Access URL: https://doaj.org/article/6af2dbed367046c6b028f7ba4d493f44
Accession Number: edsdoj.6af2dbed367046c6b028f7ba4d493f44
Database: Directory of Open Access Journals
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  Data: Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by n-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene
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  Data: <searchLink fieldCode="AR" term="%22Shuai+Huang%22">Shuai Huang</searchLink><br /><searchLink fieldCode="AR" term="%22Ying+Bi%22">Ying Bi</searchLink><br /><searchLink fieldCode="AR" term="%22Hui+Li%22">Hui Li</searchLink><br /><searchLink fieldCode="AR" term="%22Caihong+Liu%22">Caihong Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Xue+Wang%22">Xue Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Xinyu+Wang%22">Xinyu Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Yaxin+Lei%22">Yaxin Lei</searchLink><br /><searchLink fieldCode="AR" term="%22Qi+Zhang%22">Qi Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Jing+Wang%22">Jing Wang</searchLink>
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  Data: Foods, Vol 12, Iss 9, p 1904 (2023)
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  Data: MDPI AG, 2023.
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  Data: <searchLink fieldCode="DE" term="%22n-butanol%22">n-butanol</searchLink><br /><searchLink fieldCode="DE" term="%22Hami+melon%22">Hami melon</searchLink><br /><searchLink fieldCode="DE" term="%22chilling+injury%22">chilling injury</searchLink><br /><searchLink fieldCode="DE" term="%22membrane+lipid+metabolism%22">membrane lipid metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22gene+cloning%22">gene cloning</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+technology%22">Chemical technology</searchLink><br /><searchLink fieldCode="DE" term="%22TP1-1185%22">TP1-1185</searchLink>
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  Label: Description
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  Data: To investigate the effect of n-butanol on postharvest membrane lipid metabolism of Hami melon (Cucumis melo ‘Hami’), the fruits were soaked in a 1.0% solution of n-butanol for 30 min with water as the control. Symptoms of chilling injury were observed regularly, and the indices related to permeability and membrane lipid metabolism of pericarp cells were measured. The results showed that treatment with n-butanol inhibited the increase in chilling injury index, membrane permeability, and malondialdehyde content of Hami melon fruits, promoted an increase in the contents of phosphatidyl alcohol and unsaturated fatty acids, such as linoleic acid, linolenic acid, oleic acid (except 14 d), and erucic acid (28–42 d), and decreased the content of saturated fatty acids, stearic acid (0–28 d), phosphatidic acid (except for 21 d), and the key enzymes of membrane lipid metabolism compared with the control. The activities of phospholipase D (PLD) and lipoxygenase (LOX) and the downregulation of the levels of expression CmPLD-β and CmLOX (42 d only) genes reduced the chilling injury index of Hami melon and alleviated the further expansion of chilling injury symptoms in the fruits. We also cloned the key gene of membrane lipid metabolism CmPLD-β, which was obtained by pre-transcriptome screening of the pericarp. We found that CmPLD-β of Hami melon had the closest affinity with cucumber (CsXP5), indicating that the CmPLD-β gene of Hami melon was functionally similar to that of cucumber. In addition, a two-fold alignment analysis of CmPLD-β and CmXP5 base sequences indicated that the base sequences of the two promoter regions differed from each other.
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      – Text: English
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        StartPage: 1904
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      – SubjectFull: n-butanol
        Type: general
      – SubjectFull: Hami melon
        Type: general
      – SubjectFull: chilling injury
        Type: general
      – SubjectFull: membrane lipid metabolism
        Type: general
      – SubjectFull: gene cloning
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      – TitleFull: Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by n-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene
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