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Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR‐493‐3p/RAB22A axis in non‐small cell lung cancer

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Title: Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR‐493‐3p/RAB22A axis in non‐small cell lung cancer
Authors: Dongxiao Ding, Feng Yang, Zhongjie Chen, Junjie Ying
Source: Thoracic Cancer, Vol 13, Iss 4, Pp 571-581 (2022)
Publisher Information: Wiley, 2022.
Publication Year: 2022
Collection: LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Subject Terms: circ_0007385, miR‐493‐3p, non‐small cell lung cancer, RAB22A, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
More Details: Abstract Background Non‐small cell lung cancer (NSCLC) is a common cancer in the United States. Previous studies have shown that circular RNAs (circRNAs) can affect NSCLC progression, but its regulatory mechanism is still indistinct. In this study, we unfold the roles of circular RNA_0007385 in NSCLC tissues and cells. Methods Expression levels of circ_0007385, microRNA‐493‐3p (miR‐493‐3p) and Ras‐related protein Rab‐22A (RAB22A) were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR) in NSCLC tissues and cells. Cell proliferation, apoptosis and stemness were examined by cell counting kit 8 (CCK8) assay, 5‐ethynyl‐2′‐deoxyuridine (EdU) assay, flow cytometry analysis and sphere‐formation assay. The interaction between miR‐493‐3p and circ_0007385 or RAB22A was forecasted by bioinformatic analysis and detected by dual‐luciferase reporter assay, RNA immunoprecipitation (RIP) and RNA pulldown assays. In vivo experiments were implemented to verify the effect of circ_0007385 in vivo. Results Expression of circ_0007385 and RAB22A increased, whereas miR‐493‐3p level was decreased in NSCLC tissues in contrast to that in normal tissues. For functional analysis, circ_0007385 deficiency inhibited cell proliferation and stemness, whereas it promoted cell apoptosis in NSCLC cells. Mechanically, circ_0007385 acted as a miR‐493‐3p sponge to modulate RAB22A expression. Moreover, circ_0007385 could regulate the development of NSCLC by sponging miR‐493‐3p to regulate the expression of RAB22A. In addition, circ_0007385 silence also attenuated tumor growth in vivo. Conclusions Circ_0007385 promoted NSCLC progression by sponging miR‐493‐3p to increase RAB22A expression, which also offered an underlying targeted therapy for NSCLC treatment.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1759-7714
1759-7706
Relation: https://doaj.org/toc/1759-7706; https://doaj.org/toc/1759-7714
DOI: 10.1111/1759-7714.14300
Access URL: https://doaj.org/article/693d630a74894e4198dade502e8bf4b6
Accession Number: edsdoj.693d630a74894e4198dade502e8bf4b6
Database: Directory of Open Access Journals
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  Data: Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR‐493‐3p/RAB22A axis in non‐small cell lung cancer
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  Data: <searchLink fieldCode="AR" term="%22Dongxiao+Ding%22">Dongxiao Ding</searchLink><br /><searchLink fieldCode="AR" term="%22Feng+Yang%22">Feng Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Zhongjie+Chen%22">Zhongjie Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Junjie+Ying%22">Junjie Ying</searchLink>
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  Data: Thoracic Cancer, Vol 13, Iss 4, Pp 571-581 (2022)
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  Data: Wiley, 2022.
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  Data: 2022
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  Data: <searchLink fieldCode="DE" term="%22circ%5F0007385%22">circ_0007385</searchLink><br /><searchLink fieldCode="DE" term="%22miR‐493‐3p%22">miR‐493‐3p</searchLink><br /><searchLink fieldCode="DE" term="%22non‐small+cell+lung+cancer%22">non‐small cell lung cancer</searchLink><br /><searchLink fieldCode="DE" term="%22RAB22A%22">RAB22A</searchLink><br /><searchLink fieldCode="DE" term="%22Neoplasms%2E+Tumors%2E+Oncology%2E+Including+cancer+and+carcinogens%22">Neoplasms. Tumors. Oncology. Including cancer and carcinogens</searchLink><br /><searchLink fieldCode="DE" term="%22RC254-282%22">RC254-282</searchLink>
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  Label: Description
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  Data: Abstract Background Non‐small cell lung cancer (NSCLC) is a common cancer in the United States. Previous studies have shown that circular RNAs (circRNAs) can affect NSCLC progression, but its regulatory mechanism is still indistinct. In this study, we unfold the roles of circular RNA_0007385 in NSCLC tissues and cells. Methods Expression levels of circ_0007385, microRNA‐493‐3p (miR‐493‐3p) and Ras‐related protein Rab‐22A (RAB22A) were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR) in NSCLC tissues and cells. Cell proliferation, apoptosis and stemness were examined by cell counting kit 8 (CCK8) assay, 5‐ethynyl‐2′‐deoxyuridine (EdU) assay, flow cytometry analysis and sphere‐formation assay. The interaction between miR‐493‐3p and circ_0007385 or RAB22A was forecasted by bioinformatic analysis and detected by dual‐luciferase reporter assay, RNA immunoprecipitation (RIP) and RNA pulldown assays. In vivo experiments were implemented to verify the effect of circ_0007385 in vivo. Results Expression of circ_0007385 and RAB22A increased, whereas miR‐493‐3p level was decreased in NSCLC tissues in contrast to that in normal tissues. For functional analysis, circ_0007385 deficiency inhibited cell proliferation and stemness, whereas it promoted cell apoptosis in NSCLC cells. Mechanically, circ_0007385 acted as a miR‐493‐3p sponge to modulate RAB22A expression. Moreover, circ_0007385 could regulate the development of NSCLC by sponging miR‐493‐3p to regulate the expression of RAB22A. In addition, circ_0007385 silence also attenuated tumor growth in vivo. Conclusions Circ_0007385 promoted NSCLC progression by sponging miR‐493‐3p to increase RAB22A expression, which also offered an underlying targeted therapy for NSCLC treatment.
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      – SubjectFull: miR‐493‐3p
        Type: general
      – SubjectFull: non‐small cell lung cancer
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      – SubjectFull: RAB22A
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      – SubjectFull: RC254-282
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      – TitleFull: Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR‐493‐3p/RAB22A axis in non‐small cell lung cancer
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