Academic Journal
Protocol to isolate and characterize pulmonary-specific extracellular vesicles in mice
| Title: | Protocol to isolate and characterize pulmonary-specific extracellular vesicles in mice |
|---|---|
| Authors: | Nayoung Lee, Dohyun Kim, Huijae Cho, Gyeongmin Jeong, Seon-Jin Lee, Heedoo Lee |
| Source: | STAR Protocols, Vol 5, Iss 3, Pp 103183- (2024) |
| Publisher Information: | Elsevier, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Science (General) |
| Subject Terms: | Cell Biology, Cell isolation, Flow Cytometry, Molecular Biology, Science (General), Q1-390 |
| More Details: | Summary: Extracellular vesicles (EVs) are membranous nanoparticles classified based on their size and surface markers, which can be specific to various cell origins. Here, we present a protocol for the isolation of pulmonary-specific EVs in mice. We describe steps for differential centrifugation, density gradient centrifugation, and commercially available polyethylene glycol(PEG)-based precipitation, employing pulmonary-specific EV-bound chemicals and antibodies. We then detail procedures for the characterization of these EVs through nanoparticle tracking analysis, flow cytometry, scanning electron microscopy, and transmission electron microscopy.For complete details on the use and execution of this protocol, please refer to Lee et al.1,2,3,4 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2666-1667 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666166724003484; https://doaj.org/toc/2666-1667 |
| DOI: | 10.1016/j.xpro.2024.103183 |
| Access URL: | https://doaj.org/article/5aaba410c20d4d9f8c42aa87c565ae98 |
| Accession Number: | edsdoj.5aaba410c20d4d9f8c42aa87c565ae98 |
| Database: | Directory of Open Access Journals |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.xpro.2024.103183 Languages: – Text: English Subjects: – SubjectFull: Cell Biology Type: general – SubjectFull: Cell isolation Type: general – SubjectFull: Flow Cytometry Type: general – SubjectFull: Molecular Biology Type: general – SubjectFull: Science (General) Type: general – SubjectFull: Q1-390 Type: general Titles: – TitleFull: Protocol to isolate and characterize pulmonary-specific extracellular vesicles in mice Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nayoung Lee – PersonEntity: Name: NameFull: Dohyun Kim – PersonEntity: Name: NameFull: Huijae Cho – PersonEntity: Name: NameFull: Gyeongmin Jeong – PersonEntity: Name: NameFull: Seon-Jin Lee – PersonEntity: Name: NameFull: Heedoo Lee IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 26661667 Numbering: – Type: volume Value: 5 – Type: issue Value: 3 Titles: – TitleFull: STAR Protocols Type: main |
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