Academic Journal
View in EDS

Umbilical cord mesenchymal stem cells promote the repair of trochlear groove reconstruction in dogs

Bibliographic Details
Title: Umbilical cord mesenchymal stem cells promote the repair of trochlear groove reconstruction in dogs
Authors: Shi He, Jun Zhang, Wojun Chen, Yanyao Yan, Yuhong Lin, Yicheng Zhang, Shirui Lei, Chuyin Huang, Shengfeng Chen, Zhisheng Chen, Canying Liu, Yinshan Bai, Huiqin Ji, Huimin Ruan, Dongsheng Li, Cailing Ye, Cuilin Wang, Xiaoshu Zhan, Bingyun Wang
Source: Frontiers in Veterinary Science, Vol 9 (2022)
Publisher Information: Frontiers Media S.A., 2022.
Publication Year: 2022
Collection: LCC:Veterinary medicine
Subject Terms: canine, mesenchymal stem cells, patellar luxation, trochlear groove reconstruction, cartilage repair, Veterinary medicine, SF600-1100
More Details: Trochlear groove reconstruction (TGR) is a common treatment for patellar luxation (PL) in dogs. Nevertheless, the prognosis of TGR is poor due to the cartilage damage and secondary inflammation. To study the repair effect of canine umbilical cord mesenchymal stem cells (UC–MSCs) after TGR, 10 experimental dogs were given TGR surgery and then randomized into two groups: Treatment group (1 ml suspension allogeneic UC–MSCs (106 cells/kg) was injected into the cavum articulare on days 0, 7, and 14 after TGR); and the Model group (injected with 1 ml of physiological saline as negative control). The therapeutic effect of UC–MSCs was studied by blood routine examination, inflammatory factor index detection, double-blind knee score, histopathology, and computed tomography (CT) scans. The results showed that the total number of white blood cells and neutrophils in the model group were significantly higher than those in the treatment group on both 7 days and 21 days, postoperatively (P < 0.05); there were no significant changes in the levels of IL-6, MMP-13, and TGF-β1 between the model group and the treatment group throughout the days of testing. The double-blind knee scores of the treatment group were significantly lower than the model group on 1st, 4th, and 5th days postoperatively (P < 0.05). The treatment group showed low-pain sensation, stable gait, and fast recovery of muscle strength in the knee score, and the wound healing of the treatment group returned to normal on the 5th day after surgery; CT scans and gross observation showed that the cartilage growth in the treatment group was faster than that in the model group. Histological observation of cases showed that fibro chondrocytes were predominantly found in the treatment group, and the distribution of chondrocytes was uneven, while the model group showed a large number of fibrous tissue hyperplasia, fissures, and unequal matrix staining. Intra-articular injection of UC–MSCs after TGR has the effect of relieving pain and promoting the repair of bone defects, making the operative limb recover function earlier, making up for the deficiency of TGR, and improving the effect of PL treatment. Future studies should furthermore explore the dose and frequency of therapy based on the multiple advantages of UC–MSCs and the mechanism of cartilage repair in dogs.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2297-1769
Relation: https://www.frontiersin.org/articles/10.3389/fvets.2022.922390/full; https://doaj.org/toc/2297-1769
DOI: 10.3389/fvets.2022.922390
Access URL: https://doaj.org/article/544f62e8a3de474d91a62d0d39425689
Accession Number: edsdoj.544f62e8a3de474d91a62d0d39425689
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=22971769&ISBN=&volume=9&issue=&date=20220801&spage=&pages=&title=Frontiers in Veterinary Science&atitle=Umbilical%20cord%20mesenchymal%20stem%20cells%20promote%20the%20repair%20of%20trochlear%20groove%20reconstruction%20in%20dogs&aulast=Shi%20He&id=DOI:10.3389/fvets.2022.922390
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/544f62e8a3de474d91a62d0d39425689
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.544f62e8a3de474d91a62d0d39425689
RelevancyScore: 947
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 947.14111328125
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Umbilical cord mesenchymal stem cells promote the repair of trochlear groove reconstruction in dogs
– Name: Author
  Label: Authors
  Group: Au
  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Shi+He%22&quot;&gt;Shi He&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Jun+Zhang%22&quot;&gt;Jun Zhang&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wojun+Chen%22&quot;&gt;Wojun Chen&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yanyao+Yan%22&quot;&gt;Yanyao Yan&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yuhong+Lin%22&quot;&gt;Yuhong Lin&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yicheng+Zhang%22&quot;&gt;Yicheng Zhang&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Shirui+Lei%22&quot;&gt;Shirui Lei&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chuyin+Huang%22&quot;&gt;Chuyin Huang&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Shengfeng+Chen%22&quot;&gt;Shengfeng Chen&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhisheng+Chen%22&quot;&gt;Zhisheng Chen&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Canying+Liu%22&quot;&gt;Canying Liu&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yinshan+Bai%22&quot;&gt;Yinshan Bai&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Huiqin+Ji%22&quot;&gt;Huiqin Ji&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Huimin+Ruan%22&quot;&gt;Huimin Ruan&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Dongsheng+Li%22&quot;&gt;Dongsheng Li&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cailing+Ye%22&quot;&gt;Cailing Ye&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cuilin+Wang%22&quot;&gt;Cuilin Wang&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Xiaoshu+Zhan%22&quot;&gt;Xiaoshu Zhan&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Bingyun+Wang%22&quot;&gt;Bingyun Wang&lt;/searchLink&gt;
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Frontiers in Veterinary Science, Vol 9 (2022)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Frontiers Media S.A., 2022.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2022
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Veterinary medicine
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22canine%22&quot;&gt;canine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22mesenchymal+stem+cells%22&quot;&gt;mesenchymal stem cells&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22patellar+luxation%22&quot;&gt;patellar luxation&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22trochlear+groove+reconstruction%22&quot;&gt;trochlear groove reconstruction&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22cartilage+repair%22&quot;&gt;cartilage repair&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Veterinary+medicine%22&quot;&gt;Veterinary medicine&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22SF600-1100%22&quot;&gt;SF600-1100&lt;/searchLink&gt;
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Trochlear groove reconstruction (TGR) is a common treatment for patellar luxation (PL) in dogs. Nevertheless, the prognosis of TGR is poor due to the cartilage damage and secondary inflammation. To study the repair effect of canine umbilical cord mesenchymal stem cells (UC–MSCs) after TGR, 10 experimental dogs were given TGR surgery and then randomized into two groups: Treatment group (1 ml suspension allogeneic UC–MSCs (106 cells/kg) was injected into the cavum articulare on days 0, 7, and 14 after TGR); and the Model group (injected with 1 ml of physiological saline as negative control). The therapeutic effect of UC–MSCs was studied by blood routine examination, inflammatory factor index detection, double-blind knee score, histopathology, and computed tomography (CT) scans. The results showed that the total number of white blood cells and neutrophils in the model group were significantly higher than those in the treatment group on both 7 days and 21 days, postoperatively (P &lt; 0.05); there were no significant changes in the levels of IL-6, MMP-13, and TGF-β1 between the model group and the treatment group throughout the days of testing. The double-blind knee scores of the treatment group were significantly lower than the model group on 1st, 4th, and 5th days postoperatively (P &lt; 0.05). The treatment group showed low-pain sensation, stable gait, and fast recovery of muscle strength in the knee score, and the wound healing of the treatment group returned to normal on the 5th day after surgery; CT scans and gross observation showed that the cartilage growth in the treatment group was faster than that in the model group. Histological observation of cases showed that fibro chondrocytes were predominantly found in the treatment group, and the distribution of chondrocytes was uneven, while the model group showed a large number of fibrous tissue hyperplasia, fissures, and unequal matrix staining. Intra-articular injection of UC–MSCs after TGR has the effect of relieving pain and promoting the repair of bone defects, making the operative limb recover function earlier, making up for the deficiency of TGR, and improving the effect of PL treatment. Future studies should furthermore explore the dose and frequency of therapy based on the multiple advantages of UC–MSCs and the mechanism of cartilage repair in dogs.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 2297-1769
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.frontiersin.org/articles/10.3389/fvets.2022.922390/full; https://doaj.org/toc/2297-1769
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3389/fvets.2022.922390
– Name: URL
  Label: Access URL
  Group: URL
  Data: &lt;link linkTarget=&quot;URL&quot; linkTerm=&quot;https://doaj.org/article/544f62e8a3de474d91a62d0d39425689&quot; linkWindow=&quot;_blank&quot;&gt;https://doaj.org/article/544f62e8a3de474d91a62d0d39425689&lt;/link&gt;
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.544f62e8a3de474d91a62d0d39425689
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.544f62e8a3de474d91a62d0d39425689
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3389/fvets.2022.922390
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: canine
        Type: general
      – SubjectFull: mesenchymal stem cells
        Type: general
      – SubjectFull: patellar luxation
        Type: general
      – SubjectFull: trochlear groove reconstruction
        Type: general
      – SubjectFull: cartilage repair
        Type: general
      – SubjectFull: Veterinary medicine
        Type: general
      – SubjectFull: SF600-1100
        Type: general
    Titles:
      – TitleFull: Umbilical cord mesenchymal stem cells promote the repair of trochlear groove reconstruction in dogs
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Shi He
      – PersonEntity:
          Name:
            NameFull: Jun Zhang
      – PersonEntity:
          Name:
            NameFull: Wojun Chen
      – PersonEntity:
          Name:
            NameFull: Yanyao Yan
      – PersonEntity:
          Name:
            NameFull: Yuhong Lin
      – PersonEntity:
          Name:
            NameFull: Yicheng Zhang
      – PersonEntity:
          Name:
            NameFull: Shirui Lei
      – PersonEntity:
          Name:
            NameFull: Chuyin Huang
      – PersonEntity:
          Name:
            NameFull: Shengfeng Chen
      – PersonEntity:
          Name:
            NameFull: Zhisheng Chen
      – PersonEntity:
          Name:
            NameFull: Canying Liu
      – PersonEntity:
          Name:
            NameFull: Yinshan Bai
      – PersonEntity:
          Name:
            NameFull: Huiqin Ji
      – PersonEntity:
          Name:
            NameFull: Huimin Ruan
      – PersonEntity:
          Name:
            NameFull: Dongsheng Li
      – PersonEntity:
          Name:
            NameFull: Cailing Ye
      – PersonEntity:
          Name:
            NameFull: Cuilin Wang
      – PersonEntity:
          Name:
            NameFull: Xiaoshu Zhan
      – PersonEntity:
          Name:
            NameFull: Bingyun Wang
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 08
              Type: published
              Y: 2022
          Identifiers:
            – Type: issn-print
              Value: 22971769
          Numbering:
            – Type: volume
              Value: 9
          Titles:
            – TitleFull: Frontiers in Veterinary Science
              Type: main
ResultId 1