Academic Journal
View in EDS

Biosynthesis and Characterization of Zinc Oxide Nanoparticles and Their Impact on the Composition of Gut Microbiota in Healthy and Attention-Deficit Hyperactivity Disorder Children

Bibliographic Details
Title: Biosynthesis and Characterization of Zinc Oxide Nanoparticles and Their Impact on the Composition of Gut Microbiota in Healthy and Attention-Deficit Hyperactivity Disorder Children
Authors: Guoling Zhou, Rongrong Yu, Temoor Ahmed, Hubiao Jiang, Muchen Zhang, Luqiong Lv, Fahad A. Alhumaydhi, Khaled S. Allemailem, Bin Li
Source: Frontiers in Microbiology, Vol 12 (2021)
Publisher Information: Frontiers Media S.A., 2021.
Publication Year: 2021
Collection: LCC:Microbiology
Subject Terms: attention-deficit hyperactivity disorder, amplicon sequencing, bacterial community, gut bacteria, zinc oxide nanoparticles, Microbiology, QR1-502
More Details: Attention-deficit hyperactivity disorder (ADHD) seriously affects children’s health, and the gut microbiome has been widely hypothesized to play a role in the regulation of ADHD behavior. The present study aims to the biosynthesize of zinc oxide nanoparticles (ZnONPs) by using Acinetobacter johnsonii strain RTN1, followed by their characterization through state-of-the-art material characterization techniques, viz., UV–vis spectroscopy, Fourier transform infrared spectroscopy, and transmission and scanning electron microscopic analyses with energy dispersive spectrometry. Moreover, we investigated and compared the population composition of gut microbiota and their susceptibility to biogenic ZnONPs between healthy and ADHD children based on the traditional plate method and 16S rRNA amplicon sequence analysis. The antibacterial effect of ZnONPs against gut bacteria was also determined by measurement of live cell number, living/dead bacterial staining test, and flow cytometry observation. The present study revealed that the number of live gut bacteria in healthy children was more than 10-fold higher than that in ADHD children; however, the community structure of gut bacteria has changed, while greater diversity was found in gut bacteria from ADHD children. In addition, we found that the number of live gut bacteria in healthy and ADHD children was reduced by ZnONPs, which shows an increased and reduced effect in composition of gut bacteria from healthy and ADHD children, respectively. It was also noted that the main mechanism of ZnONPs may be to inhibit the growth of gut bacteria rather than to kill them, while the nanoparticle-resistant strains in healthy children is also different from that in ADHD children. Some representative bacteria, in particular nanoparticle-resistant bacteria, were successfully isolated and identified. Overall, this study revealed the potential correlation of ADHD with gut bacteria and provided a new possibility to prevent ADHD by the combination of nanoparticle and its resistant bacteria.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1664-302X
Relation: https://www.frontiersin.org/articles/10.3389/fmicb.2021.700707/full; https://doaj.org/toc/1664-302X
DOI: 10.3389/fmicb.2021.700707
Access URL: https://doaj.org/article/532a5cd960034472aed315410642b438
Accession Number: edsdoj.532a5cd960034472aed315410642b438
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=1664302X&ISBN=&volume=12&issue=&date=20210801&spage=&pages=&title=Frontiers in Microbiology&atitle=Biosynthesis%20and%20Characterization%20of%20Zinc%20Oxide%20Nanoparticles%20and%20Their%20Impact%20on%20the%20Composition%20of%20Gut%20Microbiota%20in%20Healthy%20and%20Attention-Deficit%20Hyperactivity%20Disorder%20Children&aulast=Guoling%20Zhou&id=DOI:10.3389/fmicb.2021.700707
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/532a5cd960034472aed315410642b438
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.532a5cd960034472aed315410642b438
RelevancyScore: 927
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 926.913452148438
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Biosynthesis and Characterization of Zinc Oxide Nanoparticles and Their Impact on the Composition of Gut Microbiota in Healthy and Attention-Deficit Hyperactivity Disorder Children
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Guoling+Zhou%22">Guoling Zhou</searchLink><br /><searchLink fieldCode="AR" term="%22Rongrong+Yu%22">Rongrong Yu</searchLink><br /><searchLink fieldCode="AR" term="%22Temoor+Ahmed%22">Temoor Ahmed</searchLink><br /><searchLink fieldCode="AR" term="%22Hubiao+Jiang%22">Hubiao Jiang</searchLink><br /><searchLink fieldCode="AR" term="%22Muchen+Zhang%22">Muchen Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Luqiong+Lv%22">Luqiong Lv</searchLink><br /><searchLink fieldCode="AR" term="%22Fahad+A%2E+Alhumaydhi%22">Fahad A. Alhumaydhi</searchLink><br /><searchLink fieldCode="AR" term="%22Khaled+S%2E+Allemailem%22">Khaled S. Allemailem</searchLink><br /><searchLink fieldCode="AR" term="%22Bin+Li%22">Bin Li</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Frontiers in Microbiology, Vol 12 (2021)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Frontiers Media S.A., 2021.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2021
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Microbiology
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22attention-deficit+hyperactivity+disorder%22">attention-deficit hyperactivity disorder</searchLink><br /><searchLink fieldCode="DE" term="%22amplicon+sequencing%22">amplicon sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22bacterial+community%22">bacterial community</searchLink><br /><searchLink fieldCode="DE" term="%22gut+bacteria%22">gut bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22zinc+oxide+nanoparticles%22">zinc oxide nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22QR1-502%22">QR1-502</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Attention-deficit hyperactivity disorder (ADHD) seriously affects children’s health, and the gut microbiome has been widely hypothesized to play a role in the regulation of ADHD behavior. The present study aims to the biosynthesize of zinc oxide nanoparticles (ZnONPs) by using Acinetobacter johnsonii strain RTN1, followed by their characterization through state-of-the-art material characterization techniques, viz., UV–vis spectroscopy, Fourier transform infrared spectroscopy, and transmission and scanning electron microscopic analyses with energy dispersive spectrometry. Moreover, we investigated and compared the population composition of gut microbiota and their susceptibility to biogenic ZnONPs between healthy and ADHD children based on the traditional plate method and 16S rRNA amplicon sequence analysis. The antibacterial effect of ZnONPs against gut bacteria was also determined by measurement of live cell number, living/dead bacterial staining test, and flow cytometry observation. The present study revealed that the number of live gut bacteria in healthy children was more than 10-fold higher than that in ADHD children; however, the community structure of gut bacteria has changed, while greater diversity was found in gut bacteria from ADHD children. In addition, we found that the number of live gut bacteria in healthy and ADHD children was reduced by ZnONPs, which shows an increased and reduced effect in composition of gut bacteria from healthy and ADHD children, respectively. It was also noted that the main mechanism of ZnONPs may be to inhibit the growth of gut bacteria rather than to kill them, while the nanoparticle-resistant strains in healthy children is also different from that in ADHD children. Some representative bacteria, in particular nanoparticle-resistant bacteria, were successfully isolated and identified. Overall, this study revealed the potential correlation of ADHD with gut bacteria and provided a new possibility to prevent ADHD by the combination of nanoparticle and its resistant bacteria.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1664-302X
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.frontiersin.org/articles/10.3389/fmicb.2021.700707/full; https://doaj.org/toc/1664-302X
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3389/fmicb.2021.700707
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/532a5cd960034472aed315410642b438" linkWindow="_blank">https://doaj.org/article/532a5cd960034472aed315410642b438</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.532a5cd960034472aed315410642b438
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.532a5cd960034472aed315410642b438
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3389/fmicb.2021.700707
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: attention-deficit hyperactivity disorder
        Type: general
      – SubjectFull: amplicon sequencing
        Type: general
      – SubjectFull: bacterial community
        Type: general
      – SubjectFull: gut bacteria
        Type: general
      – SubjectFull: zinc oxide nanoparticles
        Type: general
      – SubjectFull: Microbiology
        Type: general
      – SubjectFull: QR1-502
        Type: general
    Titles:
      – TitleFull: Biosynthesis and Characterization of Zinc Oxide Nanoparticles and Their Impact on the Composition of Gut Microbiota in Healthy and Attention-Deficit Hyperactivity Disorder Children
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Guoling Zhou
      – PersonEntity:
          Name:
            NameFull: Rongrong Yu
      – PersonEntity:
          Name:
            NameFull: Temoor Ahmed
      – PersonEntity:
          Name:
            NameFull: Hubiao Jiang
      – PersonEntity:
          Name:
            NameFull: Muchen Zhang
      – PersonEntity:
          Name:
            NameFull: Luqiong Lv
      – PersonEntity:
          Name:
            NameFull: Fahad A. Alhumaydhi
      – PersonEntity:
          Name:
            NameFull: Khaled S. Allemailem
      – PersonEntity:
          Name:
            NameFull: Bin Li
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 08
              Type: published
              Y: 2021
          Identifiers:
            – Type: issn-print
              Value: 1664302X
          Numbering:
            – Type: volume
              Value: 12
          Titles:
            – TitleFull: Frontiers in Microbiology
              Type: main
ResultId 1