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Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples

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Title: Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
Authors: Héctor Gabriel Avila, Marikena Guadalupe Risso, Marta Cabrera, Paula Ruybal, Silvia Analía Repetto, Marcos Javier Butti, Marcos David Trangoni, Graciela Santillán, Verónica Mirtha Pérez, María Victoria Periago
Source: Frontiers in Veterinary Science, Vol 8 (2021)
Publisher Information: Frontiers Media S.A., 2021.
Publication Year: 2021
Collection: LCC:Veterinary medicine
Subject Terms: loop mediated isothermal amplification, Ancylostoma caninum, copro-diagnosis, ancylostomiasis, molecular diagnosis, Veterinary medicine, SF600-1100
More Details: Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2297-1769
Relation: https://www.frontiersin.org/articles/10.3389/fvets.2021.770508/full; https://doaj.org/toc/2297-1769
DOI: 10.3389/fvets.2021.770508
Access URL: https://doaj.org/article/4dc72f8987ad4898937c92fd0424e038
Accession Number: edsdoj.4dc72f8987ad4898937c92fd0424e038
Database: Directory of Open Access Journals
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  Data: Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
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  Data: <searchLink fieldCode="AR" term="%22Héctor+Gabriel+Avila%22">Héctor Gabriel Avila</searchLink><br /><searchLink fieldCode="AR" term="%22Marikena+Guadalupe+Risso%22">Marikena Guadalupe Risso</searchLink><br /><searchLink fieldCode="AR" term="%22Marta+Cabrera%22">Marta Cabrera</searchLink><br /><searchLink fieldCode="AR" term="%22Paula+Ruybal%22">Paula Ruybal</searchLink><br /><searchLink fieldCode="AR" term="%22Silvia+Analía+Repetto%22">Silvia Analía Repetto</searchLink><br /><searchLink fieldCode="AR" term="%22Marcos+Javier+Butti%22">Marcos Javier Butti</searchLink><br /><searchLink fieldCode="AR" term="%22Marcos+David+Trangoni%22">Marcos David Trangoni</searchLink><br /><searchLink fieldCode="AR" term="%22Graciela+Santillán%22">Graciela Santillán</searchLink><br /><searchLink fieldCode="AR" term="%22Verónica+Mirtha+Pérez%22">Verónica Mirtha Pérez</searchLink><br /><searchLink fieldCode="AR" term="%22María+Victoria+Periago%22">María Victoria Periago</searchLink>
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  Data: Frontiers in Veterinary Science, Vol 8 (2021)
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  Data: <searchLink fieldCode="DE" term="%22loop+mediated+isothermal+amplification%22">loop mediated isothermal amplification</searchLink><br /><searchLink fieldCode="DE" term="%22Ancylostoma+caninum%22">Ancylostoma caninum</searchLink><br /><searchLink fieldCode="DE" term="%22copro-diagnosis%22">copro-diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22ancylostomiasis%22">ancylostomiasis</searchLink><br /><searchLink fieldCode="DE" term="%22molecular+diagnosis%22">molecular diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Veterinary+medicine%22">Veterinary medicine</searchLink><br /><searchLink fieldCode="DE" term="%22SF600-1100%22">SF600-1100</searchLink>
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  Data: Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.
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      – Text: English
    Subjects:
      – SubjectFull: loop mediated isothermal amplification
        Type: general
      – SubjectFull: Ancylostoma caninum
        Type: general
      – SubjectFull: copro-diagnosis
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