Identifying Antibacterial Compounds in Black Walnuts (Juglans nigra) Using a Metabolomics Approach
| Title: | Identifying Antibacterial Compounds in Black Walnuts (Juglans nigra) Using a Metabolomics Approach |
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| Authors: | Khanh-Van Ho, Zhentian Lei, Lloyd W. Sumner, Mark V. Coggeshall, Hsin-Yeh Hsieh, George C. Stewart, Chung-Ho Lin |
| Source: | Metabolites, Vol 8, Iss 4, p 58 (2018) |
| Publisher Information: | MDPI AG, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Microbiology |
| Subject Terms: | black walnut, Juglans nigra, antibacterial, metabolomics approach, compound identification, Microbiology, QR1-502 |
| More Details: | Black walnut (Juglans nigra L.) is one of the most economically valuable hardwood species and a high value tree for edible nut production in the United States. Although consumption of black walnut has been linked to multiple health-promoting effects (e.g., antioxidant, antimicrobial, anti-inflammatory), the bioactive compounds have not been systematically characterized. In addition, the associations between different black walnut cultivars and their health-promoting compounds have not been well established. In this study, the kernels of twenty-two black walnut cultivars selected for nut production by the University of Missouri Center for Agroforestry (Columbia, MO, USA) were evaluated for their antibacterial activities using agar-well diffusion assay. Among the selected cultivars, four black walnut cultivars (i.e., Mystry, Surprise, D.34, and A.36) exhibited antibacterial activity against a Gram-positive bacterium (Staphylococcus aureus), whereas other cultivars showed no effect on the inhibition of this bacterium. The antibacterial compounds showing the strongest activity were isolated with bioassay-guided purification and identified using a metabolomics approach. Six antibacterial bioactive compounds responsible for antimicrobial activity were successfully identified. Glansreginin A, azelaic acid, quercetin, and eriodictyol-7-O-glucoside are novel antibacterial compounds identified in the kernels of black walnuts. The metabolomics approach provides a simple and cost-effective tool for bioactive compound identification. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2218-1989 |
| Relation: | http://www.mdpi.com/2218-1989/8/4/58; https://doaj.org/toc/2218-1989 |
| DOI: | 10.3390/metabo8040058 |
| Access URL: | https://doaj.org/article/33b969415af24751b23f4407c12b7e6e |
| Accession Number: | edsdoj.33b969415af24751b23f4407c12b7e6e |
| Database: | Directory of Open Access Journals |
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