Academic Journal
View in EDS
PDF Full Text

Altered gene expression in blood and sputum in COPD frequent exacerbators in the ECLIPSE cohort.

Bibliographic Details
Title: Altered gene expression in blood and sputum in COPD frequent exacerbators in the ECLIPSE cohort.
Authors: Dave Singh, Steven M Fox, Ruth Tal-Singer, Stewart Bates, John H Riley, Bartolome Celli
Source: PLoS ONE, Vol 9, Iss 9, p e107381 (2014)
Publisher Information: Public Library of Science (PLoS), 2014.
Publication Year: 2014
Collection: LCC:Medicine
LCC:Science
Subject Terms: Medicine, Science
More Details: Patients with chronic obstructive pulmonary disease (COPD) who are defined as frequent exacerbators suffer with 2 or more exacerbations every year. The molecular mechanisms responsible for this phenotype are poorly understood. We investigated gene expression profile patterns associated with frequent exacerbations in sputum and blood cells in a well-characterised cohort. Samples from subjects from the ECLIPSE COPD cohort were used; sputum and blood samples from 138 subjects were used for microarray gene expression analysis, while blood samples from 438 subjects were used for polymerase chain reaction (PCR) testing. Using microarray, 150 genes were differentially expressed in blood (>±1.5 fold change, p≤0.01) between frequent compared to non-exacerbators. In sputum cells, only 6 genes were differentially expressed. The differentially regulated genes in blood included downregulation of those involved in lymphocyte signalling and upregulation of pro-apoptotic signalling genes. Multivariate analysis of the microarray data followed by confirmatory PCR analysis identified 3 genes that predicted frequent exacerbations; B3GNT, LAF4 and ARHGEF10. The sensitivity and specificity of these 3 genes to predict the frequent exacerbator phenotype was 88% and 33% respectively. There are alterations in systemic immune function associated with frequent exacerbations; down-regulation of lymphocyte function and a shift towards pro-apoptosis mechanisms are apparent in patients with frequent exacerbations.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1932-6203
Relation: http://europepmc.org/articles/PMC4179270?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0107381
Access URL: https://doaj.org/article/1c0423a38f0b4d4e9a50d134537d7e3a
Accession Number: edsdoj.1c0423a38f0b4d4e9a50d134537d7e3a
Database: Directory of Open Access Journals
FullText Links:
  – Type: pdflink
    Url: https://content.ebscohost.com/cds/retrieve?content=AQICAHjPtM4BHU3ZchRwgzYmadcigk49r9CVlbU7V5F6lgH7WwFDFyivIZjl5j2FL-0zvtIqAAAA4TCB3gYJKoZIhvcNAQcGoIHQMIHNAgEAMIHHBgkqhkiG9w0BBwEwHgYJYIZIAWUDBAEuMBEEDCPlup6bjF_Yta5xLQIBEICBmXxd_FntfaR_jw-Lt5n5hwol-Fy1_kaHtnn5kVlVYUfK6ThmRimocQpGJ_M_KvIfnzuKJmal2UHUdUt9RPIFg3gNQneEK8CpRmNqTbJTQsvLPMad8j0358DqytUdH4bq57-3SZopkPFNjU91pxdvaQ2Be5NFmaEAaAIannsWynRKHnHVntDYzO0CxGA1sTthhSJObe3oSBETzw==
Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=19326203&ISBN=&volume=9&issue=9&date=20140101&spage=e107381&pages=&title=PLoS ONE&atitle=Altered%20gene%20expression%20in%20blood%20and%20sputum%20in%20COPD%20frequent%20exacerbators%20in%20the%20ECLIPSE%20cohort.&aulast=Dave%20Singh&id=DOI:10.1371/journal.pone.0107381
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/1c0423a38f0b4d4e9a50d134537d7e3a
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.1c0423a38f0b4d4e9a50d134537d7e3a
RelevancyScore: 848
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 848.219055175781
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Altered gene expression in blood and sputum in COPD frequent exacerbators in the ECLIPSE cohort.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Dave+Singh%22">Dave Singh</searchLink><br /><searchLink fieldCode="AR" term="%22Steven+M+Fox%22">Steven M Fox</searchLink><br /><searchLink fieldCode="AR" term="%22Ruth+Tal-Singer%22">Ruth Tal-Singer</searchLink><br /><searchLink fieldCode="AR" term="%22Stewart+Bates%22">Stewart Bates</searchLink><br /><searchLink fieldCode="AR" term="%22John+H+Riley%22">John H Riley</searchLink><br /><searchLink fieldCode="AR" term="%22Bartolome+Celli%22">Bartolome Celli</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: PLoS ONE, Vol 9, Iss 9, p e107381 (2014)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Public Library of Science (PLoS), 2014.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2014
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Medicine<br />LCC:Science
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Medicine%22">Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Science%22">Science</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Patients with chronic obstructive pulmonary disease (COPD) who are defined as frequent exacerbators suffer with 2 or more exacerbations every year. The molecular mechanisms responsible for this phenotype are poorly understood. We investigated gene expression profile patterns associated with frequent exacerbations in sputum and blood cells in a well-characterised cohort. Samples from subjects from the ECLIPSE COPD cohort were used; sputum and blood samples from 138 subjects were used for microarray gene expression analysis, while blood samples from 438 subjects were used for polymerase chain reaction (PCR) testing. Using microarray, 150 genes were differentially expressed in blood (>±1.5 fold change, p≤0.01) between frequent compared to non-exacerbators. In sputum cells, only 6 genes were differentially expressed. The differentially regulated genes in blood included downregulation of those involved in lymphocyte signalling and upregulation of pro-apoptotic signalling genes. Multivariate analysis of the microarray data followed by confirmatory PCR analysis identified 3 genes that predicted frequent exacerbations; B3GNT, LAF4 and ARHGEF10. The sensitivity and specificity of these 3 genes to predict the frequent exacerbator phenotype was 88% and 33% respectively. There are alterations in systemic immune function associated with frequent exacerbations; down-regulation of lymphocyte function and a shift towards pro-apoptosis mechanisms are apparent in patients with frequent exacerbations.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1932-6203
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: http://europepmc.org/articles/PMC4179270?pdf=render; https://doaj.org/toc/1932-6203
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1371/journal.pone.0107381
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/1c0423a38f0b4d4e9a50d134537d7e3a" linkWindow="_blank">https://doaj.org/article/1c0423a38f0b4d4e9a50d134537d7e3a</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.1c0423a38f0b4d4e9a50d134537d7e3a
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.1c0423a38f0b4d4e9a50d134537d7e3a
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1371/journal.pone.0107381
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        StartPage: e107381
    Subjects:
      – SubjectFull: Medicine
        Type: general
      – SubjectFull: Science
        Type: general
    Titles:
      – TitleFull: Altered gene expression in blood and sputum in COPD frequent exacerbators in the ECLIPSE cohort.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Dave Singh
      – PersonEntity:
          Name:
            NameFull: Steven M Fox
      – PersonEntity:
          Name:
            NameFull: Ruth Tal-Singer
      – PersonEntity:
          Name:
            NameFull: Stewart Bates
      – PersonEntity:
          Name:
            NameFull: John H Riley
      – PersonEntity:
          Name:
            NameFull: Bartolome Celli
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Type: published
              Y: 2014
          Identifiers:
            – Type: issn-print
              Value: 19326203
          Numbering:
            – Type: volume
              Value: 9
            – Type: issue
              Value: 9
          Titles:
            – TitleFull: PLoS ONE
              Type: main
ResultId 1