Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering
| Title: | Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering |
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| Authors: | Stefania Croce, Lorenzo Cobianchi, Tamara Zoro, Francesca Dal Mas, Antonia Icaro Cornaglia, Elisa Lenta, Gloria Acquafredda, Annalisa De Silvestri, Maria Antonietta Avanzini, Livia Visai, Szandra Brambilla, Giovanna Bruni, Giulia Di Gravina, Andrea Pietrabissa, Luca Ansaloni, Andrea Peloso |
| Source: | Biomedicines, Vol 10, Iss 11, p 2817 (2022) |
| Publisher Information: | MDPI AG, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Biology (General) |
| Subject Terms: | mesenchymal stromal cells, bioscaffold, decellularisation, extracellular matrix, recellularisation, tissue engineering, Biology (General), QH301-705.5 |
| More Details: | Background: In end-stage chronic liver disease, transplantation represents the only curative option. However, the shortage of donors results in the death of many patients. To overcome this gap, it is mandatory to develop new therapeutic options. In the present study, we decellularised pig livers and reseeded them with allogeneic porcine mesenchymal stromal cells (pMSCs) to understand whether extracellular matrix (ECM) can influence and/or promote differentiation into hepatocyte-like cells (HLCs). Methods: After decellularisation with SDS, the integrity of ECM-scaffolds was examined by histological staining, immunofluorescence and scanning electron microscope. DNA quantification was used to assess decellularisation. pMSCs were plated on scaffolds by static seeding and maintained in in vitro culture for 21 days. At 3, 7, 14 and 21 days, seeded ECM scaffolds were evaluated for cellular adhesion and growth. Moreover, the expression of specific hepatic genes was performed by RT-PCR. Results: The applied decellularisation/recellularisation protocol was effective. The number of seeded pMSCs increased over the culture time points. Gene expression analysis of seeded pMSCs displayed a weak induction due to ECM towards HLCs. Conclusions: These results suggest that ECM may address pMSCs to differentiate in hepatocyte-like cells. However, only contact with liver-ECM is not enough to induce complete differentiation. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2227-9059 13692720 |
| Relation: | https://www.mdpi.com/2227-9059/10/11/2817; https://doaj.org/toc/2227-9059 |
| DOI: | 10.3390/biomedicines10112817 |
| Access URL: | https://doaj.org/article/c13692720d514f8f9ed025ab0c95ad23 |
| Accession Number: | edsdoj.13692720d514f8f9ed025ab0c95ad23 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22279059 13692720 |
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| DOI: | 10.3390/biomedicines10112817 |
| Published in: | Biomedicines |
| Language: | English |