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PACAP modulates L‐type Ca2+channel currents in vascular smooth muscle cells: involvement of PKC and PKA

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Title: PACAP modulates L‐type Ca2+channel currents in vascular smooth muscle cells: involvement of PKC and PKA
Authors: Chik, Constance L., Li, Bing, Ogiwara, Takayuki, Ho, Anthony K., Karpinski, Edward
Source: The FASEB Journal; September 1996, Vol. 10 Issue: 11 p1310-1317, 8p
Abstract: The effect of pituitary adenylate cyclase activating polypeptide (PACAP) on the L‐type Ca2+channel current (L‐channel current) was studied in smooth muscle cells prepared from the rat tail artery. PACAP caused an increase in the amplitude of the L‐channel current. The maximal increase (56%) occurred at a PACAP concentration of 1 X 10−8M; higher concentrations resulted in a smaller increase. Investigation into the intracellular mecha‐nisms of PACAP action revealed that the increase in L‐channel currents was blocked by calphostin C and bisindolylmaleimide IV [protein kinase C (PKC) inhibitors] and mimicked by 4β‐phorbol 12‐myristate 13‐acetate (PMA), an activator of PKC. PACAP was also found to cause translocation of PKC, suggesting that the increase in the current by PACAP was due to PKC. In contrast, activation of cAMP‐dependent protein kinase (PKA) by 8‐bromo‐cAMP caused an inhibition of the L‐channel current. A high concentration of PACAP (1 times 10−6M) had no effect on the L‐channel current. The null effect of PACAP on the L‐channel current could be converted to an increase by Rp‐cAMPs, a cAMP antagonist, and a decrease by calphostin C. PACAP also increased cAMP accumulation. These observations indicate the effect of PACAP on the L‐channel current represents the integration of two signaling mechanisms that involve the activation of PKA and PKC.—Chik, C. L., Li, B., Ogiwara, T., Ho, A. K., Karpinski, E. PACAP modulates L‐type Ca2+channel currents in vascular smooth muscle cells: involvement of PKC and PKA. FASEB J.10, 1310‐1317 (1996)
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  Label: Title
  Group: Ti
  Data: PACAP modulates L‐type Ca2+channel currents in vascular smooth muscle cells: involvement of PKC and PKA
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Chik%2C+Constance+L%2E%22">Chik, Constance L.</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Bing%22">Li, Bing</searchLink><br /><searchLink fieldCode="AR" term="%22Ogiwara%2C+Takayuki%22">Ogiwara, Takayuki</searchLink><br /><searchLink fieldCode="AR" term="%22Ho%2C+Anthony+K%2E%22">Ho, Anthony K.</searchLink><br /><searchLink fieldCode="AR" term="%22Karpinski%2C+Edward%22">Karpinski, Edward</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: The FASEB Journal; September 1996, Vol. 10 Issue: 11 p1310-1317, 8p
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The effect of pituitary adenylate cyclase activating polypeptide (PACAP) on the L‐type Ca2+channel current (L‐channel current) was studied in smooth muscle cells prepared from the rat tail artery. PACAP caused an increase in the amplitude of the L‐channel current. The maximal increase (56%) occurred at a PACAP concentration of 1 X 10−8M; higher concentrations resulted in a smaller increase. Investigation into the intracellular mecha‐nisms of PACAP action revealed that the increase in L‐channel currents was blocked by calphostin C and bisindolylmaleimide IV [protein kinase C (PKC) inhibitors] and mimicked by 4β‐phorbol 12‐myristate 13‐acetate (PMA), an activator of PKC. PACAP was also found to cause translocation of PKC, suggesting that the increase in the current by PACAP was due to PKC. In contrast, activation of cAMP‐dependent protein kinase (PKA) by 8‐bromo‐cAMP caused an inhibition of the L‐channel current. A high concentration of PACAP (1 times 10−6M) had no effect on the L‐channel current. The null effect of PACAP on the L‐channel current could be converted to an increase by Rp‐cAMPs, a cAMP antagonist, and a decrease by calphostin C. PACAP also increased cAMP accumulation. These observations indicate the effect of PACAP on the L‐channel current represents the integration of two signaling mechanisms that involve the activation of PKA and PKC.—Chik, C. L., Li, B., Ogiwara, T., Ho, A. K., Karpinski, E. PACAP modulates L‐type Ca2+channel currents in vascular smooth muscle cells: involvement of PKC and PKA. FASEB J.10, 1310‐1317 (1996)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1096/fasebj.10.11.8836045
    Languages:
      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 1310
    Titles:
      – TitleFull: PACAP modulates L‐type Ca2+channel currents in vascular smooth muscle cells: involvement of PKC and PKA
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      – PersonEntity:
          Name:
            NameFull: Chik, Constance L.
      – PersonEntity:
          Name:
            NameFull: Li, Bing
      – PersonEntity:
          Name:
            NameFull: Ogiwara, Takayuki
      – PersonEntity:
          Name:
            NameFull: Ho, Anthony K.
      – PersonEntity:
          Name:
            NameFull: Karpinski, Edward
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          Dates:
            – D: 01
              M: 09
              Text: September 1996
              Type: published
              Y: 1996
          Identifiers:
            – Type: issn-print
              Value: 08926638
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              Value: 15306860
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              Value: 10
            – Type: issue
              Value: 11
          Titles:
            – TitleFull: The FASEB Journal
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