Periodical
View in EDS

Relationship between donor animal age, follicular fluid steroid content and oocyte developmental competence in the pig

Bibliographic Details
Title: Relationship between donor animal age, follicular fluid steroid content and oocyte developmental competence in the pig
Authors: Grupen, Christopher G., McIlfatrick, Stephen M., Ashman, Rodney J., Boquest, Andrew C., Armstrong, David T., Nottle, Mark B.
Source: Reproduction, Fertility and Development; 2003, Vol. 15 Issue: 2 p81-87, 7p
Abstract: The developmental competence of oocytes recovered from the ovaries of slaughtered prepubertal and adult pigs was evaluated after in vitro maturation, parthenogenetic activation and culture in vitro. In addition, the effect of prepubertal and adult follicular fluid (FF) on the developmental competence of prepubertal and adult oocytes was investigated. When matured in adult FF, the rates of cleavage (92 v. 73% P < 0.01) and blastocyst formation (57 v. 38%; P < 0.05) were greater for adult oocytes than for prepubertal oocytes. Blastocysts derived from adult oocytes had more trophectoderm cells (43 v. 30; P < 0.05) and total cells (51 v. 36; P < 0.05) than blastocysts derived from prepubertal oocytes. The developmental competence of prepubertal oocytes was not affected by the FF donor age, whereas the developmental competence of adult oocytes was. Blastocysts derived from adult oocytes matured in adult FF had more trophectoderm cells (38 v. 24; P < 0.005), inner cell mass cells (7 v. 3; P < 0.01) and total cells (45 v. 27; P < 0.001) than blastocysts derived from adult oocytes matured in prepubertal FF. Characterization of the steroid content of the FF used to supplement the maturation medium revealed that adult FF contained more progesterone (42 v. 23 ng mL–1; P < 0.005) and androstenedione (70 v. 16 ng mL–1; P < 0.05) than prepubertal FF. In addition, the molar ratios of progesterone to androstenedione, androstenedione to 17β-oestradiol and androstenedione to testosterone differed (P < 0.05) between prepubertal and adult FF. The results support the hypothesis that a greater proportion of adult oocytes than of prepubertal oocytes has completed 'oocyte capacitation'. The differences in FF steroid content are indicative of the different follicular environments from which the prepubertal and adult oocytes were isolated, and may be attributed to the observed effects on oocyte developmental competence.
Database: Supplemental Index
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edo&genre=article&issn=10313613&ISBN=&volume=15&issue=2&date=20030101&spage=81&pages=81-87&title=Reproduction, Fertility and Development&atitle=Relationship%20between%20donor%20animal%20age%2C%20follicular%20fluid%20steroid%20content%20and%20oocyte%20developmental%20competence%20in%20the%20pig&aulast=Grupen%2C%20Christopher%20G.&id=DOI:
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
Header DbId: edo
DbLabel: Supplemental Index
An: ejs4637730
RelevancyScore: 817
AccessLevel: 6
PubType: Periodical
PubTypeId: serialPeriodical
PreciseRelevancyScore: 816.759887695313
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Relationship between donor animal age, follicular fluid steroid content and oocyte developmental competence in the pig
– Name: Author
  Label: Authors
  Group: Au
  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Grupen%2C+Christopher+G%2E%22&quot;&gt;Grupen, Christopher G.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22McIlfatrick%2C+Stephen+M%2E%22&quot;&gt;McIlfatrick, Stephen M.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Ashman%2C+Rodney+J%2E%22&quot;&gt;Ashman, Rodney J.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Boquest%2C+Andrew+C%2E%22&quot;&gt;Boquest, Andrew C.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Armstrong%2C+David+T%2E%22&quot;&gt;Armstrong, David T.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Nottle%2C+Mark+B%2E%22&quot;&gt;Nottle, Mark B.&lt;/searchLink&gt;
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Reproduction, Fertility and Development; 2003, Vol. 15 Issue: 2 p81-87, 7p
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The developmental competence of oocytes recovered from the ovaries of slaughtered prepubertal and adult pigs was evaluated after in vitro maturation, parthenogenetic activation and culture in vitro. In addition, the effect of prepubertal and adult follicular fluid (FF) on the developmental competence of prepubertal and adult oocytes was investigated. When matured in adult FF, the rates of cleavage (92 v. 73% P &lt; 0.01) and blastocyst formation (57 v. 38%; P &lt; 0.05) were greater for adult oocytes than for prepubertal oocytes. Blastocysts derived from adult oocytes had more trophectoderm cells (43 v. 30; P &lt; 0.05) and total cells (51 v. 36; P &lt; 0.05) than blastocysts derived from prepubertal oocytes. The developmental competence of prepubertal oocytes was not affected by the FF donor age, whereas the developmental competence of adult oocytes was. Blastocysts derived from adult oocytes matured in adult FF had more trophectoderm cells (38 v. 24; P &lt; 0.005), inner cell mass cells (7 v. 3; P &lt; 0.01) and total cells (45 v. 27; P &lt; 0.001) than blastocysts derived from adult oocytes matured in prepubertal FF. Characterization of the steroid content of the FF used to supplement the maturation medium revealed that adult FF contained more progesterone (42 v. 23 ng mL–1; P &lt; 0.005) and androstenedione (70 v. 16 ng mL–1; P &lt; 0.05) than prepubertal FF. In addition, the molar ratios of progesterone to androstenedione, androstenedione to 17β-oestradiol and androstenedione to testosterone differed (P &lt; 0.05) between prepubertal and adult FF. The results support the hypothesis that a greater proportion of adult oocytes than of prepubertal oocytes has completed &#39;oocyte capacitation&#39;. The differences in FF steroid content are indicative of the different follicular environments from which the prepubertal and adult oocytes were isolated, and may be attributed to the observed effects on oocyte developmental competence.
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edo&AN=ejs4637730
RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 81
    Titles:
      – TitleFull: Relationship between donor animal age, follicular fluid steroid content and oocyte developmental competence in the pig
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Grupen, Christopher G.
      – PersonEntity:
          Name:
            NameFull: McIlfatrick, Stephen M.
      – PersonEntity:
          Name:
            NameFull: Ashman, Rodney J.
      – PersonEntity:
          Name:
            NameFull: Boquest, Andrew C.
      – PersonEntity:
          Name:
            NameFull: Armstrong, David T.
      – PersonEntity:
          Name:
            NameFull: Nottle, Mark B.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Text: 2003
              Type: published
              Y: 2003
          Identifiers:
            – Type: issn-print
              Value: 10313613
          Numbering:
            – Type: volume
              Value: 15
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: Reproduction, Fertility and Development
              Type: main
ResultId 1