Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus.
| Title: | Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus. |
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| Authors: | Eun-Ju Kim, Kwang-Myun Cheong, Ha-Kyung Joung, Bo-Hye Kim, Jae-Young Song, In-Soo Cho, Kyoung-Ki Lee, Yeun-Kyung Shin |
| Source: | Journal of Veterinary Science; Dec2016, Vol. 17 Issue 4, p479-487, 9p |
| Subject Terms: | BOVINE leukemia virus, LEUKEMIA in animals, PRELEUKEMIA, IMMUNOGLOBULINS, PLASMA cells |
| Abstract: | Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the development of an immunochromatographic assay to detect BLV antibodies in cattle. The sensitivity and specificity of the assay were determined by comparing these results with those obtained from a standard enzyme linked immunosorbent assay (ELISA). A total of 160 bovine sera were used to evaluate the new immunochromatographic assay. Using ELISA as a reference standard, the relative specificity and sensitivity of this assay were determined to be 94.7% and 98%, respectively. Because of its high sensitivity and specificity, this BLV antibody detection assay would be suitable for the onsite identification of BLV infection in the field. [ABSTRACT FROM AUTHOR] |
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| Database: | Supplemental Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Eun-Ju+Kim%22">Eun-Ju Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Kwang-Myun+Cheong%22">Kwang-Myun Cheong</searchLink><br /><searchLink fieldCode="AR" term="%22Ha-Kyung+Joung%22">Ha-Kyung Joung</searchLink><br /><searchLink fieldCode="AR" term="%22Bo-Hye+Kim%22">Bo-Hye Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Jae-Young+Song%22">Jae-Young Song</searchLink><br /><searchLink fieldCode="AR" term="%22In-Soo+Cho%22">In-Soo Cho</searchLink><br /><searchLink fieldCode="AR" term="%22Kyoung-Ki+Lee%22">Kyoung-Ki Lee</searchLink><br /><searchLink fieldCode="AR" term="%22Yeun-Kyung+Shin%22">Yeun-Kyung Shin</searchLink> – Name: TitleSource Label: Source Group: Src Data: Journal of Veterinary Science; Dec2016, Vol. 17 Issue 4, p479-487, 9p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22BOVINE+leukemia+virus%22">BOVINE leukemia virus</searchLink><br /><searchLink fieldCode="DE" term="%22LEUKEMIA+in+animals%22">LEUKEMIA in animals</searchLink><br /><searchLink fieldCode="DE" term="%22PRELEUKEMIA%22">PRELEUKEMIA</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNOGLOBULINS%22">IMMUNOGLOBULINS</searchLink><br /><searchLink fieldCode="DE" term="%22PLASMA+cells%22">PLASMA cells</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the development of an immunochromatographic assay to detect BLV antibodies in cattle. The sensitivity and specificity of the assay were determined by comparing these results with those obtained from a standard enzyme linked immunosorbent assay (ELISA). A total of 160 bovine sera were used to evaluate the new immunochromatographic assay. Using ELISA as a reference standard, the relative specificity and sensitivity of this assay were determined to be 94.7% and 98%, respectively. Because of its high sensitivity and specificity, this BLV antibody detection assay would be suitable for the onsite identification of BLV infection in the field. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Journal of Veterinary Science is the property of Korean Society of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.4142/jvs.2016.17.4.479 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 479 Subjects: – SubjectFull: BOVINE leukemia virus Type: general – SubjectFull: LEUKEMIA in animals Type: general – SubjectFull: PRELEUKEMIA Type: general – SubjectFull: IMMUNOGLOBULINS Type: general – SubjectFull: PLASMA cells Type: general Titles: – TitleFull: Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Eun-Ju Kim – PersonEntity: Name: NameFull: Kwang-Myun Cheong – PersonEntity: Name: NameFull: Ha-Kyung Joung – PersonEntity: Name: NameFull: Bo-Hye Kim – PersonEntity: Name: NameFull: Jae-Young Song – PersonEntity: Name: NameFull: In-Soo Cho – PersonEntity: Name: NameFull: Kyoung-Ki Lee – PersonEntity: Name: NameFull: Yeun-Kyung Shin IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 1229845X Numbering: – Type: volume Value: 17 – Type: issue Value: 4 Titles: – TitleFull: Journal of Veterinary Science Type: main |
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