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Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes.

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Title: Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes.
Authors: Kuan-Hung Lin, Yan-Yin Yang, Chi-Ming Yang, Meng-Yuan Huang, Hsiao-Feng Lo, Kuang-Chuan Liu, Hwei-Shen Lin, Pi-Yu Chao
Source: BMC Research Notes; 2013, Vol. 6 Issue 1, p1-19, 19p, 4 Charts, 2 Graphs
Subject Terms: ANTIOXIDANTS, DNA damage, HERBACEOUS plants, PLANT extracts, OXIDATIVE stress, HYDROGEN peroxide, THERAPEUTICS
Abstract: Background Herbaceous plants containing antioxidants can protect against DNA damage. The purpose of this study was to evaluate the antioxidant substances, antioxidant activity, and protection of DNA from oxidative damage in human lymphocytes induced by hydrogen peroxide (H2O2). Our methods used acidic methanol and water extractions from six herbaceous plants, including Bidens alba (BA), Lycium chinense (LC), Mentha arvensis (MA), Plantago asiatica (PA), Houttuynia cordata (HC), and Centella asiatica (CA). Methods Antioxidant compounds such as flavonol and polyphenol were analyzed. Antioxidant activity was determined by the inhibition percentage of conjugated diene formation in a linoleic acid emulsion system and by trolox-equivalent antioxidant capacity (TEAC) assay. Their antioxidative capacities for protecting human lymphocyte DNA from H2O2-induced strand breaks was evaluated by comet assay. Results The studied plants were found to be rich in flavonols, especially myricetin in BA, morin in MA, quercetin in HC, and kaemperol in CA. In addition, polyphenol abounded in BA and CA. The best conjugated diene formation inhibition percentage was found in the acidic methanolic extract of PA. Regarding TEAC, the best antioxidant activity was generated from the acidic methanolic extract of HC. Water and acidic methanolic extracts of MA and HC both had better inhibition percentages of tail DNA% and tail moment as compared to the rest of the tested extracts, and significantly suppressed oxidative damage to lymphocyte DNA. Conclusion Quercetin and morin are important for preventing peroxidation and oxidative damage to DNA, and the leaves of MA and HC extracts may have excellent potential as functional ingredients representing potential sources of natural antioxidants. [ABSTRACT FROM AUTHOR]
Copyright of BMC Research Notes is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes.
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  Data: <searchLink fieldCode="AR" term="%22Kuan-Hung+Lin%22">Kuan-Hung Lin</searchLink><br /><searchLink fieldCode="AR" term="%22Yan-Yin+Yang%22">Yan-Yin Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Chi-Ming+Yang%22">Chi-Ming Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Meng-Yuan+Huang%22">Meng-Yuan Huang</searchLink><br /><searchLink fieldCode="AR" term="%22Hsiao-Feng+Lo%22">Hsiao-Feng Lo</searchLink><br /><searchLink fieldCode="AR" term="%22Kuang-Chuan+Liu%22">Kuang-Chuan Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Hwei-Shen+Lin%22">Hwei-Shen Lin</searchLink><br /><searchLink fieldCode="AR" term="%22Pi-Yu+Chao%22">Pi-Yu Chao</searchLink>
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  Data: BMC Research Notes; 2013, Vol. 6 Issue 1, p1-19, 19p, 4 Charts, 2 Graphs
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22ANTIOXIDANTS%22">ANTIOXIDANTS</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22HERBACEOUS+plants%22">HERBACEOUS plants</searchLink><br /><searchLink fieldCode="DE" term="%22PLANT+extracts%22">PLANT extracts</searchLink><br /><searchLink fieldCode="DE" term="%22OXIDATIVE+stress%22">OXIDATIVE stress</searchLink><br /><searchLink fieldCode="DE" term="%22HYDROGEN+peroxide%22">HYDROGEN peroxide</searchLink><br /><searchLink fieldCode="DE" term="%22THERAPEUTICS%22">THERAPEUTICS</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Background Herbaceous plants containing antioxidants can protect against DNA damage. The purpose of this study was to evaluate the antioxidant substances, antioxidant activity, and protection of DNA from oxidative damage in human lymphocytes induced by hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>). Our methods used acidic methanol and water extractions from six herbaceous plants, including Bidens alba (BA), Lycium chinense (LC), Mentha arvensis (MA), Plantago asiatica (PA), Houttuynia cordata (HC), and Centella asiatica (CA). Methods Antioxidant compounds such as flavonol and polyphenol were analyzed. Antioxidant activity was determined by the inhibition percentage of conjugated diene formation in a linoleic acid emulsion system and by trolox-equivalent antioxidant capacity (TEAC) assay. Their antioxidative capacities for protecting human lymphocyte DNA from H<subscript>2</subscript>O<subscript>2</subscript>-induced strand breaks was evaluated by comet assay. Results The studied plants were found to be rich in flavonols, especially myricetin in BA, morin in MA, quercetin in HC, and kaemperol in CA. In addition, polyphenol abounded in BA and CA. The best conjugated diene formation inhibition percentage was found in the acidic methanolic extract of PA. Regarding TEAC, the best antioxidant activity was generated from the acidic methanolic extract of HC. Water and acidic methanolic extracts of MA and HC both had better inhibition percentages of tail DNA% and tail moment as compared to the rest of the tested extracts, and significantly suppressed oxidative damage to lymphocyte DNA. Conclusion Quercetin and morin are important for preventing peroxidation and oxidative damage to DNA, and the leaves of MA and HC extracts may have excellent potential as functional ingredients representing potential sources of natural antioxidants. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of BMC Research Notes is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1186/1756-0500-6-490
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      – Code: eng
        Text: English
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        PageCount: 19
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      – SubjectFull: ANTIOXIDANTS
        Type: general
      – SubjectFull: DNA damage
        Type: general
      – SubjectFull: HERBACEOUS plants
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      – SubjectFull: PLANT extracts
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      – SubjectFull: HYDROGEN peroxide
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              Text: 2013
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