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Enhanced invasion and survival of antibiotic- resistant Klebsiella pneumoniae pathotypes in host cells and strain-specific replication in blood.

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Title: Enhanced invasion and survival of antibiotic- resistant Klebsiella pneumoniae pathotypes in host cells and strain-specific replication in blood.
Authors: Klaper, Kathleen, Pfeifer, Yvonne, Heinrich, Lena, Prax, Marcel, Krut, Oleg, Bekeredjian-Ding, Isabelle, Wahl, Anika, Fischer, Martin A., Kaspar, Heike, Borgmann, Stefan, Gerlach, Roman G., Werner, Guido
Source: Frontiers in Cellular & Infection Microbiology; 2025, p1-16, 16p
Subject Terms: CELL morphology, KLEBSIELLA pneumoniae, STATISTICAL significance, COMMUNITY-acquired infections, HUMAN origins
Abstract: Background: Klebsiella pneumoniae is one of the most important opportunistic pathogens causing healthcare-associated and community-acquired infections worldwide. In recent years, the increase in antibiotic resistance and infections caused by hypervirulent K. pneumoniae poses great public health concerns. In this study, host-pathogen interactions of different K. pneumoniae strains of human and animal origins were analyzed in microbiological, cell-biological and immunological experiments. Methods: In vitro infection experiments using representatives of different K. pneumoniae pathotypes and various epithelial and macrophage cell lines were executed analyzing adhesion, invasion and intracellular replication. Experimental conditions involved normoxia and hypoxia. Furthermore, survival and growth of further K. pneumoniae isolates expressing defined siderophores in blood (platelet concentrates, serum) was investigated. All experiments were done in triplicate and statistically significant differences were determined. Results: Significant differences in adhesion and invasion capability, phagocytosis resistance and intracellular replication were measured between different K. pneumoniae pathotypes. Especially, ESBL-producing K. pneumoniae isolates demonstrated increased invasion in host cell lines and survival in macrophages. A strong cytotoxic effect on intestinal cells was observed for hypervirulent K. pneumoniae. The results from our investigations of the growth behavior of K. pneumoniae in platelets and serum showed that siderophores and/or an enlarged capsule are not essential factors for the proliferation of (hypervirulent) K. pneumoniae strains in blood components. Conclusion: Our in vitro experiments revealed new insights into the host-pathogen interactions of K. pneumoniae strains representing different pathovars and clonal lineages in different infectious contexts and hosts. While a clear limitation of our study is the limited strain set used for both infection and as potential host, the results are a further step for a better understanding of the pathogenicity of K. pneumoniae and its properties essential for different stages of colonization and infection. When developed further, these results may offer novel approaches for future therapeutics including novel "anti-virulence strategies". [ABSTRACT FROM AUTHOR]
Copyright of Frontiers in Cellular & Infection Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Enhanced invasion and survival of antibiotic- resistant Klebsiella pneumoniae pathotypes in host cells and strain-specific replication in blood.
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  Data: <searchLink fieldCode="AR" term="%22Klaper%2C+Kathleen%22">Klaper, Kathleen</searchLink><br /><searchLink fieldCode="AR" term="%22Pfeifer%2C+Yvonne%22">Pfeifer, Yvonne</searchLink><br /><searchLink fieldCode="AR" term="%22Heinrich%2C+Lena%22">Heinrich, Lena</searchLink><br /><searchLink fieldCode="AR" term="%22Prax%2C+Marcel%22">Prax, Marcel</searchLink><br /><searchLink fieldCode="AR" term="%22Krut%2C+Oleg%22">Krut, Oleg</searchLink><br /><searchLink fieldCode="AR" term="%22Bekeredjian-Ding%2C+Isabelle%22">Bekeredjian-Ding, Isabelle</searchLink><br /><searchLink fieldCode="AR" term="%22Wahl%2C+Anika%22">Wahl, Anika</searchLink><br /><searchLink fieldCode="AR" term="%22Fischer%2C+Martin+A%2E%22">Fischer, Martin A.</searchLink><br /><searchLink fieldCode="AR" term="%22Kaspar%2C+Heike%22">Kaspar, Heike</searchLink><br /><searchLink fieldCode="AR" term="%22Borgmann%2C+Stefan%22">Borgmann, Stefan</searchLink><br /><searchLink fieldCode="AR" term="%22Gerlach%2C+Roman+G%2E%22">Gerlach, Roman G.</searchLink><br /><searchLink fieldCode="AR" term="%22Werner%2C+Guido%22">Werner, Guido</searchLink>
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  Label: Source
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  Data: Frontiers in Cellular & Infection Microbiology; 2025, p1-16, 16p
– Name: Subject
  Label: Subject Terms
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  Data: <searchLink fieldCode="DE" term="%22CELL+morphology%22">CELL morphology</searchLink><br /><searchLink fieldCode="DE" term="%22KLEBSIELLA+pneumoniae%22">KLEBSIELLA pneumoniae</searchLink><br /><searchLink fieldCode="DE" term="%22STATISTICAL+significance%22">STATISTICAL significance</searchLink><br /><searchLink fieldCode="DE" term="%22COMMUNITY-acquired+infections%22">COMMUNITY-acquired infections</searchLink><br /><searchLink fieldCode="DE" term="%22HUMAN+origins%22">HUMAN origins</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Background: Klebsiella pneumoniae is one of the most important opportunistic pathogens causing healthcare-associated and community-acquired infections worldwide. In recent years, the increase in antibiotic resistance and infections caused by hypervirulent K. pneumoniae poses great public health concerns. In this study, host-pathogen interactions of different K. pneumoniae strains of human and animal origins were analyzed in microbiological, cell-biological and immunological experiments. Methods: In vitro infection experiments using representatives of different K. pneumoniae pathotypes and various epithelial and macrophage cell lines were executed analyzing adhesion, invasion and intracellular replication. Experimental conditions involved normoxia and hypoxia. Furthermore, survival and growth of further K. pneumoniae isolates expressing defined siderophores in blood (platelet concentrates, serum) was investigated. All experiments were done in triplicate and statistically significant differences were determined. Results: Significant differences in adhesion and invasion capability, phagocytosis resistance and intracellular replication were measured between different K. pneumoniae pathotypes. Especially, ESBL-producing K. pneumoniae isolates demonstrated increased invasion in host cell lines and survival in macrophages. A strong cytotoxic effect on intestinal cells was observed for hypervirulent K. pneumoniae. The results from our investigations of the growth behavior of K. pneumoniae in platelets and serum showed that siderophores and/or an enlarged capsule are not essential factors for the proliferation of (hypervirulent) K. pneumoniae strains in blood components. Conclusion: Our in vitro experiments revealed new insights into the host-pathogen interactions of K. pneumoniae strains representing different pathovars and clonal lineages in different infectious contexts and hosts. While a clear limitation of our study is the limited strain set used for both infection and as potential host, the results are a further step for a better understanding of the pathogenicity of K. pneumoniae and its properties essential for different stages of colonization and infection. When developed further, these results may offer novel approaches for future therapeutics including novel "anti-virulence strategies". [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Frontiers in Cellular & Infection Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Text: English
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        PageCount: 16
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      – SubjectFull: CELL morphology
        Type: general
      – SubjectFull: KLEBSIELLA pneumoniae
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      – SubjectFull: STATISTICAL significance
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              Text: 2025
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