Development, Characterization, and Evaluation of Chi-Tn mAb-Functionalized DOTAP-PLGA Hybrid Nanoparticles Loaded with Docetaxel for Lung Cancer Therapy.
| Title: | Development, Characterization, and Evaluation of Chi-Tn mAb-Functionalized DOTAP-PLGA Hybrid Nanoparticles Loaded with Docetaxel for Lung Cancer Therapy. |
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| Authors: | Castro, Analía, Pittini, Álvaro, Berois, Nora, Faccio, Ricardo, Miranda, Pablo, Mombrú, Álvaro W., Osinaga, Eduardo, Pardo, Helena |
| Source: | Pharmaceutics; Feb2025, Vol. 17 Issue 2, p164, 16p |
| Subject Terms: | ATOMIC force microscopy, TRANSMISSION electron microscopy, SURFACE charges, COLLOIDAL stability, LIGHT scattering, RAMAN microscopy |
| Abstract: | Background/Objectives: The focus of this study was to prepare and characterize docetaxel (DCX)-loaded lipid/polymer hybrid nanoparticles (LPHNps) functionalized with the monoclonal antibody (mAb) Chi-Tn for a potential active targeting approach in lung cancer treatment. Methods: We synthesized DOTAP-PLGA hybrid nanoparticles loaded with DCX and functionalized them with Chi-Tn mAb through a biotin–avidin approach. The physicochemical characterization involved dynamic light scattering, transmission electron microscopy, Raman spectroscopy, and atomic force microscopy. The in vitro and in vivo evaluations encompassed uptake studies, cell viability tests, and the assessment of tumor growth control in a lung cancer model. Results: The nanoparticles featured a hydrophobic PLGA core with 99.9% DCX encapsulation efficiency, surrounded by a DOTAP lipid shell ensuring colloidal stability with a high positive surface charge. The incorporation of PEGylated lipids on their surface helps evade the immune system and facilitate Chi-Tn mAb attachment. The resulting nanoparticles exhibit a spherical shape with monodisperse particle sizes averaging 250 nm, and demonstrate sustained drug release. In vitro uptake studies and viability assays conducted in A549 cancer cells show that the Chi-Tn mAb enhances nanoparticle internalization and significantly reduces cell viability. In vivo studies demonstrate a notable reduction in tumor volume and an increased survival rate in the A549 tumor xenograft mice model when DCX was encapsulated in nanoparticles and targeted with Chi-Tn mAb in comparison to the free drug. Conclusions: Therefore, Chi-Tn-functionalized LPHNps hold promise as carriers for actively targeting DCX to Tn-expressing carcinomas. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Header | DbId: edb DbLabel: Complementary Index An: 183334519 RelevancyScore: 1082 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1082.12268066406 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development, Characterization, and Evaluation of Chi-Tn mAb-Functionalized DOTAP-PLGA Hybrid Nanoparticles Loaded with Docetaxel for Lung Cancer Therapy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Castro%2C+Analía%22">Castro, Analía</searchLink><br /><searchLink fieldCode="AR" term="%22Pittini%2C+Álvaro%22">Pittini, Álvaro</searchLink><br /><searchLink fieldCode="AR" term="%22Berois%2C+Nora%22">Berois, Nora</searchLink><br /><searchLink fieldCode="AR" term="%22Faccio%2C+Ricardo%22">Faccio, Ricardo</searchLink><br /><searchLink fieldCode="AR" term="%22Miranda%2C+Pablo%22">Miranda, Pablo</searchLink><br /><searchLink fieldCode="AR" term="%22Mombrú%2C+Álvaro+W%2E%22">Mombrú, Álvaro W.</searchLink><br /><searchLink fieldCode="AR" term="%22Osinaga%2C+Eduardo%22">Osinaga, Eduardo</searchLink><br /><searchLink fieldCode="AR" term="%22Pardo%2C+Helena%22">Pardo, Helena</searchLink> – Name: TitleSource Label: Source Group: Src Data: Pharmaceutics; Feb2025, Vol. 17 Issue 2, p164, 16p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22ATOMIC+force+microscopy%22">ATOMIC force microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22TRANSMISSION+electron+microscopy%22">TRANSMISSION electron microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22SURFACE+charges%22">SURFACE charges</searchLink><br /><searchLink fieldCode="DE" term="%22COLLOIDAL+stability%22">COLLOIDAL stability</searchLink><br /><searchLink fieldCode="DE" term="%22LIGHT+scattering%22">LIGHT scattering</searchLink><br /><searchLink fieldCode="DE" term="%22RAMAN+microscopy%22">RAMAN microscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background/Objectives: The focus of this study was to prepare and characterize docetaxel (DCX)-loaded lipid/polymer hybrid nanoparticles (LPHNps) functionalized with the monoclonal antibody (mAb) Chi-Tn for a potential active targeting approach in lung cancer treatment. Methods: We synthesized DOTAP-PLGA hybrid nanoparticles loaded with DCX and functionalized them with Chi-Tn mAb through a biotin–avidin approach. The physicochemical characterization involved dynamic light scattering, transmission electron microscopy, Raman spectroscopy, and atomic force microscopy. The in vitro and in vivo evaluations encompassed uptake studies, cell viability tests, and the assessment of tumor growth control in a lung cancer model. Results: The nanoparticles featured a hydrophobic PLGA core with 99.9% DCX encapsulation efficiency, surrounded by a DOTAP lipid shell ensuring colloidal stability with a high positive surface charge. The incorporation of PEGylated lipids on their surface helps evade the immune system and facilitate Chi-Tn mAb attachment. The resulting nanoparticles exhibit a spherical shape with monodisperse particle sizes averaging 250 nm, and demonstrate sustained drug release. In vitro uptake studies and viability assays conducted in A549 cancer cells show that the Chi-Tn mAb enhances nanoparticle internalization and significantly reduces cell viability. In vivo studies demonstrate a notable reduction in tumor volume and an increased survival rate in the A549 tumor xenograft mice model when DCX was encapsulated in nanoparticles and targeted with Chi-Tn mAb in comparison to the free drug. Conclusions: Therefore, Chi-Tn-functionalized LPHNps hold promise as carriers for actively targeting DCX to Tn-expressing carcinomas. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Pharmaceutics is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/pharmaceutics17020164 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 16 StartPage: 164 Subjects: – SubjectFull: ATOMIC force microscopy Type: general – SubjectFull: TRANSMISSION electron microscopy Type: general – SubjectFull: SURFACE charges Type: general – SubjectFull: COLLOIDAL stability Type: general – SubjectFull: LIGHT scattering Type: general – SubjectFull: RAMAN microscopy Type: general Titles: – TitleFull: Development, Characterization, and Evaluation of Chi-Tn mAb-Functionalized DOTAP-PLGA Hybrid Nanoparticles Loaded with Docetaxel for Lung Cancer Therapy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Castro, Analía – PersonEntity: Name: NameFull: Pittini, Álvaro – PersonEntity: Name: NameFull: Berois, Nora – PersonEntity: Name: NameFull: Faccio, Ricardo – PersonEntity: Name: NameFull: Miranda, Pablo – PersonEntity: Name: NameFull: Mombrú, Álvaro W. – PersonEntity: Name: NameFull: Osinaga, Eduardo – PersonEntity: Name: NameFull: Pardo, Helena IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 19994923 Numbering: – Type: volume Value: 17 – Type: issue Value: 2 Titles: – TitleFull: Pharmaceutics Type: main |
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