Subinhibitory concentrations of antibiotics affect development and parameters of Helicobacter pylori biofilm.
| Title: | Subinhibitory concentrations of antibiotics affect development and parameters of Helicobacter pylori biofilm. |
|---|---|
| Authors: | Krzyżek, Paweł, Migdał, Paweł, Tusiewicz, Kaja, Zawadzki, Marcin, Szpot, Paweł |
| Source: | Frontiers in Pharmacology; 2024, p1-19, 19p |
| Subject Terms: | HELICOBACTER pylori, GASTRIC diseases, DISEASE relapse, FATTY acids, CLARITHROMYCIN |
| Abstract: | Introduction: Helicobacter pylori causes chronic gastric diseases in nearly 50% of people around the world. It is suggested that biofilm formation has a pronounced effect on the dynamic resistance spread and recurrence of these infections. Methods: To mimic the scenario of therapeutic ineffectiveness, we investigated the impact of sub-minimal inhibitory concentrations (sub-MICs) of antibiotics on the development and parameters of biofilms produced by clinical H. pylori strains. Results: We observed that constant exposure of planktonic forms to metronidazole or levofloxacin stimulated the speed of autoaggregation and the amount of extracellular matrix, resulting in increased dimensions of the developed biofilms. Contrary to this, continuous exposure to clarithromycin negatively affected a number of biofilm-related reactions and led to the biofilm-weakening effect. Through assessing the membrane fatty acid profiles of antibiotic-exposed cells, we confirmed that metronidazole and levofloxacin induced a biofilm-like phenotype, while clarithromycin kept bacteria in a planktonic form. Discussion: Our results suggest that sub-MICs of antibiotics affect the biochemical and biophysical properties of the developing biofilm of H. pylori strains and may impact the effectiveness of antibiotic treatment. [ABSTRACT FROM AUTHOR] |
| Copyright of Frontiers in Pharmacology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edb&genre=article&issn=16639812&ISBN=&volume=&issue=&date=20241028&spage=1&pages=1-19&title=Frontiers in Pharmacology&atitle=Subinhibitory%20concentrations%20of%20antibiotics%20affect%20development%20and%20parameters%20of%20Helicobacter%20pylori%20biofilm.&aulast=Krzy%C5%BCek%2C%20Pawe%C5%82&id=DOI:10.3389/fphar.2024.1477317 Name: Full Text Finder (for New FTF UI) (s8985755) Category: fullText Text: Find It @ SCU Libraries MouseOverText: Find It @ SCU Libraries |
|---|---|
| Header | DbId: edb DbLabel: Complementary Index An: 180542464 RelevancyScore: 1041 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1040.52294921875 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Subinhibitory concentrations of antibiotics affect development and parameters of Helicobacter pylori biofilm. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Krzyżek%2C+Paweł%22">Krzyżek, Paweł</searchLink><br /><searchLink fieldCode="AR" term="%22Migdał%2C+Paweł%22">Migdał, Paweł</searchLink><br /><searchLink fieldCode="AR" term="%22Tusiewicz%2C+Kaja%22">Tusiewicz, Kaja</searchLink><br /><searchLink fieldCode="AR" term="%22Zawadzki%2C+Marcin%22">Zawadzki, Marcin</searchLink><br /><searchLink fieldCode="AR" term="%22Szpot%2C+Paweł%22">Szpot, Paweł</searchLink> – Name: TitleSource Label: Source Group: Src Data: Frontiers in Pharmacology; 2024, p1-19, 19p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22HELICOBACTER+pylori%22">HELICOBACTER pylori</searchLink><br /><searchLink fieldCode="DE" term="%22GASTRIC+diseases%22">GASTRIC diseases</searchLink><br /><searchLink fieldCode="DE" term="%22DISEASE+relapse%22">DISEASE relapse</searchLink><br /><searchLink fieldCode="DE" term="%22FATTY+acids%22">FATTY acids</searchLink><br /><searchLink fieldCode="DE" term="%22CLARITHROMYCIN%22">CLARITHROMYCIN</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Introduction: Helicobacter pylori causes chronic gastric diseases in nearly 50% of people around the world. It is suggested that biofilm formation has a pronounced effect on the dynamic resistance spread and recurrence of these infections. Methods: To mimic the scenario of therapeutic ineffectiveness, we investigated the impact of sub-minimal inhibitory concentrations (sub-MICs) of antibiotics on the development and parameters of biofilms produced by clinical H. pylori strains. Results: We observed that constant exposure of planktonic forms to metronidazole or levofloxacin stimulated the speed of autoaggregation and the amount of extracellular matrix, resulting in increased dimensions of the developed biofilms. Contrary to this, continuous exposure to clarithromycin negatively affected a number of biofilm-related reactions and led to the biofilm-weakening effect. Through assessing the membrane fatty acid profiles of antibiotic-exposed cells, we confirmed that metronidazole and levofloxacin induced a biofilm-like phenotype, while clarithromycin kept bacteria in a planktonic form. Discussion: Our results suggest that sub-MICs of antibiotics affect the biochemical and biophysical properties of the developing biofilm of H. pylori strains and may impact the effectiveness of antibiotic treatment. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Frontiers in Pharmacology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edb&AN=180542464 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3389/fphar.2024.1477317 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 1 Subjects: – SubjectFull: HELICOBACTER pylori Type: general – SubjectFull: GASTRIC diseases Type: general – SubjectFull: DISEASE relapse Type: general – SubjectFull: FATTY acids Type: general – SubjectFull: CLARITHROMYCIN Type: general Titles: – TitleFull: Subinhibitory concentrations of antibiotics affect development and parameters of Helicobacter pylori biofilm. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Krzyżek, Paweł – PersonEntity: Name: NameFull: Migdał, Paweł – PersonEntity: Name: NameFull: Tusiewicz, Kaja – PersonEntity: Name: NameFull: Zawadzki, Marcin – PersonEntity: Name: NameFull: Szpot, Paweł IsPartOfRelationships: – BibEntity: Dates: – D: 28 M: 10 Text: 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 16639812 Titles: – TitleFull: Frontiers in Pharmacology Type: main |
| ResultId | 1 |