Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model.
| Title: | Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model. |
|---|---|
| Authors: | Rodríguez-Expósito, Rubén L., Sifaoui, Ines, Salazar-Villatoro, Lizbeth, Bethencourt-Estrella, Carlos J., Fernández, José J., Díaz-Marrero, Ana R., Sutak, Robert, Omaña-Molina, Maritza, Piñero, José E., Lorenzo-Morales, Jacob |
| Source: | Marine Drugs; Sep2024, Vol. 22 Issue 9, p423, 12p |
| Abstract: | Acanthamoeba is a ubiquitous genus of amoebae that can trigger a severe and progressive ocular disease known as Acanthamoeba Keratitis (AK). Furthermore, current treatment protocols are based on the combination of different compounds that are not fully effective. Therefore, an urgent need to find new compounds to treat Acanthamoeba infections is clear. In the present study, we evaluated staurosporine as a potential treatment for Acanthamoeba keratitis using mouse cornea as an ex vivo model, and a comparative proteomic analysis was conducted to elucidate a mechanism of action. The obtained results indicate that staurosporine altered the conformation of actin and tubulin in treated trophozoites of A. castellanii. In addition, proteomic analysis of treated trophozoites revealed that this molecule induced overexpression and a downregulation of proteins related to key functions for Acanthamoeba infection pathways. Additionally, the ex vivo assay used validated this model for the study of the pathogenesis and therapies of AK. Finally, staurosporine eliminated the entire amoebic population and prevented the adhesion and infection of amoebae to the epithelium of treated mouse corneas. [ABSTRACT FROM AUTHOR] |
| Copyright of Marine Drugs is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edb&genre=article&issn=16603397&ISBN=&volume=22&issue=9&date=20240901&spage=423&pages=423-434&title=Marine Drugs&atitle=Staurosporine%20as%20a%20Potential%20Treatment%20for%20Acanthamoeba%20Keratitis%20Using%20Mouse%20Cornea%20as%20an%20Ex%20Vivo%20Model.&aulast=Rodr%C3%ADguez-Exp%C3%B3sito%2C%20Rub%C3%A9n%20L.&id=DOI:10.3390/md22090423 Name: Full Text Finder (for New FTF UI) (s8985755) Category: fullText Text: Find It @ SCU Libraries MouseOverText: Find It @ SCU Libraries |
|---|---|
| Header | DbId: edb DbLabel: Complementary Index An: 179964517 RelevancyScore: 1041 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1040.51928710938 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Rodríguez-Expósito%2C+Rubén+L%2E%22">Rodríguez-Expósito, Rubén L.</searchLink><br /><searchLink fieldCode="AR" term="%22Sifaoui%2C+Ines%22">Sifaoui, Ines</searchLink><br /><searchLink fieldCode="AR" term="%22Salazar-Villatoro%2C+Lizbeth%22">Salazar-Villatoro, Lizbeth</searchLink><br /><searchLink fieldCode="AR" term="%22Bethencourt-Estrella%2C+Carlos+J%2E%22">Bethencourt-Estrella, Carlos J.</searchLink><br /><searchLink fieldCode="AR" term="%22Fernández%2C+José+J%2E%22">Fernández, José J.</searchLink><br /><searchLink fieldCode="AR" term="%22Díaz-Marrero%2C+Ana+R%2E%22">Díaz-Marrero, Ana R.</searchLink><br /><searchLink fieldCode="AR" term="%22Sutak%2C+Robert%22">Sutak, Robert</searchLink><br /><searchLink fieldCode="AR" term="%22Omaña-Molina%2C+Maritza%22">Omaña-Molina, Maritza</searchLink><br /><searchLink fieldCode="AR" term="%22Piñero%2C+José+E%2E%22">Piñero, José E.</searchLink><br /><searchLink fieldCode="AR" term="%22Lorenzo-Morales%2C+Jacob%22">Lorenzo-Morales, Jacob</searchLink> – Name: TitleSource Label: Source Group: Src Data: Marine Drugs; Sep2024, Vol. 22 Issue 9, p423, 12p – Name: Abstract Label: Abstract Group: Ab Data: Acanthamoeba is a ubiquitous genus of amoebae that can trigger a severe and progressive ocular disease known as Acanthamoeba Keratitis (AK). Furthermore, current treatment protocols are based on the combination of different compounds that are not fully effective. Therefore, an urgent need to find new compounds to treat Acanthamoeba infections is clear. In the present study, we evaluated staurosporine as a potential treatment for Acanthamoeba keratitis using mouse cornea as an ex vivo model, and a comparative proteomic analysis was conducted to elucidate a mechanism of action. The obtained results indicate that staurosporine altered the conformation of actin and tubulin in treated trophozoites of A. castellanii. In addition, proteomic analysis of treated trophozoites revealed that this molecule induced overexpression and a downregulation of proteins related to key functions for Acanthamoeba infection pathways. Additionally, the ex vivo assay used validated this model for the study of the pathogenesis and therapies of AK. Finally, staurosporine eliminated the entire amoebic population and prevented the adhesion and infection of amoebae to the epithelium of treated mouse corneas. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Marine Drugs is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edb&AN=179964517 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/md22090423 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 423 Titles: – TitleFull: Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Rodríguez-Expósito, Rubén L. – PersonEntity: Name: NameFull: Sifaoui, Ines – PersonEntity: Name: NameFull: Salazar-Villatoro, Lizbeth – PersonEntity: Name: NameFull: Bethencourt-Estrella, Carlos J. – PersonEntity: Name: NameFull: Fernández, José J. – PersonEntity: Name: NameFull: Díaz-Marrero, Ana R. – PersonEntity: Name: NameFull: Sutak, Robert – PersonEntity: Name: NameFull: Omaña-Molina, Maritza – PersonEntity: Name: NameFull: Piñero, José E. – PersonEntity: Name: NameFull: Lorenzo-Morales, Jacob IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 16603397 Numbering: – Type: volume Value: 22 – Type: issue Value: 9 Titles: – TitleFull: Marine Drugs Type: main |
| ResultId | 1 |