Laurencia johnstonii extract reverses early lesions in the K14E7HPV16 murine cervical carcinogenesis model.
| Title: | Laurencia johnstonii extract reverses early lesions in the K14E7HPV16 murine cervical carcinogenesis model. |
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| Authors: | Arvizu‐Hernandez, Erandi, Hernandez‐Guerrero, Claudia Judith, Alvarez‐Rios, Elizabeth, Gariglio, Patricio, Cornejo‐Garrido, Jorge, Ocadiz‐Delgado, Rodolfo |
| Source: | Journal of Medical Virology; Apr2024, Vol. 96 Issue 4, p1-13, 13p |
| Subject Terms: | CERVICAL intraepithelial neoplasia, GENE expression, HUMAN papillomavirus, TRANSGENIC mice, HUMAN carcinogenesis, CELL determination, NECK pain |
| Abstract: | Persistent infection with high‐risk human papillomavirus (HR‐HPV) is a well‐established risk factor to the development of cervical intraepithelial neoplasia (CIN), a condition that can progress to cervical cancer (CC) a major health problem worldwide. Recently, there has been growing interest in exploring alternative therapies utilizing natural products, among which is the algae species Laurencia johnstonii Setchell & Gardner, 1924 (L. johnstonii), proposed for the management of precancerous lesions. The aim of this work was to determine the effect of an organic extract from L. johnstonii (ELj) in early cervical lesions (CIN 1). These CIN 1 lesions were generated in a murine model expressing the HR‐HPV16 E7 oncoprotein (K14E7HPV transgenic mice) with a single exogenous hormonal stimulus using 17β‐estradiol. The histopathological studies, the determination of cell proliferation and of the apoptotic levels in cervical tissue, showed that, seven doses of ELj (30 mg/kg weight per day diluted in a DMSO‐saline solution [1:7]) lead to recovery the architecture of cervical epithelium. Accordingly, in the transgenic mice it was observed a statistically significant decrease of the PCNA expression levels, a marker of cell proliferation, and a statistically significant increase in the apoptosis levels using Caspase 3 as a marker. In addition, we determined the expression levels of the tumor suppressor miR‐218 and the oncomiRNA miR‐21. Interestingly, our results may suggest that ELj treatment tended to restore the normal expression of both miRNAs as compared with controls being more evident in the non‐transgenic induced mice. Differences of p < 0.05 were considered statistically significant through the whole study. Based on these results, we propose that the use of ELj could be an alternative for the treatment of cervical early lesions. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Laurencia johnstonii extract reverses early lesions in the K14E7HPV16 murine cervical carcinogenesis model. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Arvizu‐Hernandez%2C+Erandi%22">Arvizu‐Hernandez, Erandi</searchLink><br /><searchLink fieldCode="AR" term="%22Hernandez‐Guerrero%2C+Claudia+Judith%22">Hernandez‐Guerrero, Claudia Judith</searchLink><br /><searchLink fieldCode="AR" term="%22Alvarez‐Rios%2C+Elizabeth%22">Alvarez‐Rios, Elizabeth</searchLink><br /><searchLink fieldCode="AR" term="%22Gariglio%2C+Patricio%22">Gariglio, Patricio</searchLink><br /><searchLink fieldCode="AR" term="%22Cornejo‐Garrido%2C+Jorge%22">Cornejo‐Garrido, Jorge</searchLink><br /><searchLink fieldCode="AR" term="%22Ocadiz‐Delgado%2C+Rodolfo%22">Ocadiz‐Delgado, Rodolfo</searchLink> – Name: TitleSource Label: Source Group: Src Data: Journal of Medical Virology; Apr2024, Vol. 96 Issue 4, p1-13, 13p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22CERVICAL+intraepithelial+neoplasia%22">CERVICAL intraepithelial neoplasia</searchLink><br /><searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink><br /><searchLink fieldCode="DE" term="%22HUMAN+papillomavirus%22">HUMAN papillomavirus</searchLink><br /><searchLink fieldCode="DE" term="%22TRANSGENIC+mice%22">TRANSGENIC mice</searchLink><br /><searchLink fieldCode="DE" term="%22HUMAN+carcinogenesis%22">HUMAN carcinogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+determination%22">CELL determination</searchLink><br /><searchLink fieldCode="DE" term="%22NECK+pain%22">NECK pain</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Persistent infection with high‐risk human papillomavirus (HR‐HPV) is a well‐established risk factor to the development of cervical intraepithelial neoplasia (CIN), a condition that can progress to cervical cancer (CC) a major health problem worldwide. Recently, there has been growing interest in exploring alternative therapies utilizing natural products, among which is the algae species Laurencia johnstonii Setchell & Gardner, 1924 (L. johnstonii), proposed for the management of precancerous lesions. The aim of this work was to determine the effect of an organic extract from L. johnstonii (ELj) in early cervical lesions (CIN 1). These CIN 1 lesions were generated in a murine model expressing the HR‐HPV16 E7 oncoprotein (K14E7HPV transgenic mice) with a single exogenous hormonal stimulus using 17β‐estradiol. The histopathological studies, the determination of cell proliferation and of the apoptotic levels in cervical tissue, showed that, seven doses of ELj (30 mg/kg weight per day diluted in a DMSO‐saline solution [1:7]) lead to recovery the architecture of cervical epithelium. Accordingly, in the transgenic mice it was observed a statistically significant decrease of the PCNA expression levels, a marker of cell proliferation, and a statistically significant increase in the apoptosis levels using Caspase 3 as a marker. In addition, we determined the expression levels of the tumor suppressor miR‐218 and the oncomiRNA miR‐21. Interestingly, our results may suggest that ELj treatment tended to restore the normal expression of both miRNAs as compared with controls being more evident in the non‐transgenic induced mice. Differences of p < 0.05 were considered statistically significant through the whole study. Based on these results, we propose that the use of ELj could be an alternative for the treatment of cervical early lesions. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Journal of Medical Virology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/jmv.29571 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1 Subjects: – SubjectFull: CERVICAL intraepithelial neoplasia Type: general – SubjectFull: GENE expression Type: general – SubjectFull: HUMAN papillomavirus Type: general – SubjectFull: TRANSGENIC mice Type: general – SubjectFull: HUMAN carcinogenesis Type: general – SubjectFull: CELL determination Type: general – SubjectFull: NECK pain Type: general Titles: – TitleFull: Laurencia johnstonii extract reverses early lesions in the K14E7HPV16 murine cervical carcinogenesis model. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Arvizu‐Hernandez, Erandi – PersonEntity: Name: NameFull: Hernandez‐Guerrero, Claudia Judith – PersonEntity: Name: NameFull: Alvarez‐Rios, Elizabeth – PersonEntity: Name: NameFull: Gariglio, Patricio – PersonEntity: Name: NameFull: Cornejo‐Garrido, Jorge – PersonEntity: Name: NameFull: Ocadiz‐Delgado, Rodolfo IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 01466615 Numbering: – Type: volume Value: 96 – Type: issue Value: 4 Titles: – TitleFull: Journal of Medical Virology Type: main |
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