Unremitting pro‐inflammatory T‐cell phenotypes, and macrophage activity, following paediatric burn injury.
| Title: | Unremitting pro‐inflammatory T‐cell phenotypes, and macrophage activity, following paediatric burn injury. |
|---|---|
| Authors: | Langley, Donna, Zimmermann, Kate, Krenske, Emma, Stefanutti, Giorgio, Kimble, Roy M, Holland, Andrew JA, Fear, Mark W, Wood, Fiona M, Kenna, Tony, Cuttle, Leila |
| Source: | Clinical & Translational Immunology; 2024, Vol. 13 Issue 3, p1-14, 14p |
| Subject Terms: | MONONUCLEAR leukocytes, KILLER cells, T cells, REGULATORY T cells, PEDIATRICS |
| Abstract: | Objectives: The aim of this study was to characterise the dynamic immune profile of paediatric burn patients for up to 18 months post‐burn. Methods: Flow cytometry was used to measure 25 cell markers, chemokines and cytokines which reflected both pro‐inflammatory and anti‐inflammatory immune profiles. Peripheral blood mononuclear cells from 6 paediatric burn patients who had returned for repeated burn and scar treatments for > 4 timepoints within 12 months post‐burn were compared to four age‐matched healthy controls. Results: While overall proportions of T cells, NK cells and macrophages remained relatively constant, over time percentages of these immune cells differentiated into effector and proinflammatory cell phenotypes including Th17 and activated γδ T cells. Circulating proportions of γδ T cells increased their expression of pro‐inflammatory mediators throughout the burn recovery, with a 3–6 fold increase of IL‐17 at 1–3 weeks, and NFκβ 9–18 months post‐burn. T‐regulatory cell plasticity was also observed, and Treg phenotype proportions changed from systemically reduced skin‐homing T‐regs (CCR4+) and increased inflammatory (CCR6+) at 1‐month post‐burn, to double‐positive cell types (CCR4+CCR6+) elevated in circulation for 18 months post‐burn. Furthermore, Tregs were observed to proportionally express less IL‐10 but increased TNF‐α over 18 months. Conclusion: Overall, these results indicate the circulating percentages of immune cells do not increase or decrease over time post‐burn, instead they become highly specialised, inflammatory and skin‐homing. In this patient population, these changes persisted for at least 18 months post‐burn, this 'immune distraction' may limit the ability of immune cells to prioritise other threats post‐burn, such as respiratory infections. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Header | DbId: edb DbLabel: Complementary Index An: 176213980 RelevancyScore: 1007 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1007.03765869141 |
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| Items | – Name: Title Label: Title Group: Ti Data: Unremitting pro‐inflammatory T‐cell phenotypes, and macrophage activity, following paediatric burn injury. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Langley%2C+Donna%22">Langley, Donna</searchLink><br /><searchLink fieldCode="AR" term="%22Zimmermann%2C+Kate%22">Zimmermann, Kate</searchLink><br /><searchLink fieldCode="AR" term="%22Krenske%2C+Emma%22">Krenske, Emma</searchLink><br /><searchLink fieldCode="AR" term="%22Stefanutti%2C+Giorgio%22">Stefanutti, Giorgio</searchLink><br /><searchLink fieldCode="AR" term="%22Kimble%2C+Roy+M%22">Kimble, Roy M</searchLink><br /><searchLink fieldCode="AR" term="%22Holland%2C+Andrew+JA%22">Holland, Andrew JA</searchLink><br /><searchLink fieldCode="AR" term="%22Fear%2C+Mark+W%22">Fear, Mark W</searchLink><br /><searchLink fieldCode="AR" term="%22Wood%2C+Fiona+M%22">Wood, Fiona M</searchLink><br /><searchLink fieldCode="AR" term="%22Kenna%2C+Tony%22">Kenna, Tony</searchLink><br /><searchLink fieldCode="AR" term="%22Cuttle%2C+Leila%22">Cuttle, Leila</searchLink> – Name: TitleSource Label: Source Group: Src Data: Clinical & Translational Immunology; 2024, Vol. 13 Issue 3, p1-14, 14p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22MONONUCLEAR+leukocytes%22">MONONUCLEAR leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22KILLER+cells%22">KILLER cells</searchLink><br /><searchLink fieldCode="DE" term="%22T+cells%22">T cells</searchLink><br /><searchLink fieldCode="DE" term="%22REGULATORY+T+cells%22">REGULATORY T cells</searchLink><br /><searchLink fieldCode="DE" term="%22PEDIATRICS%22">PEDIATRICS</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Objectives: The aim of this study was to characterise the dynamic immune profile of paediatric burn patients for up to 18 months post‐burn. Methods: Flow cytometry was used to measure 25 cell markers, chemokines and cytokines which reflected both pro‐inflammatory and anti‐inflammatory immune profiles. Peripheral blood mononuclear cells from 6 paediatric burn patients who had returned for repeated burn and scar treatments for > 4 timepoints within 12 months post‐burn were compared to four age‐matched healthy controls. Results: While overall proportions of T cells, NK cells and macrophages remained relatively constant, over time percentages of these immune cells differentiated into effector and proinflammatory cell phenotypes including Th17 and activated γδ T cells. Circulating proportions of γδ T cells increased their expression of pro‐inflammatory mediators throughout the burn recovery, with a 3–6 fold increase of IL‐17 at 1–3 weeks, and NFκβ 9–18 months post‐burn. T‐regulatory cell plasticity was also observed, and Treg phenotype proportions changed from systemically reduced skin‐homing T‐regs (CCR4+) and increased inflammatory (CCR6+) at 1‐month post‐burn, to double‐positive cell types (CCR4+CCR6+) elevated in circulation for 18 months post‐burn. Furthermore, Tregs were observed to proportionally express less IL‐10 but increased TNF‐α over 18 months. Conclusion: Overall, these results indicate the circulating percentages of immune cells do not increase or decrease over time post‐burn, instead they become highly specialised, inflammatory and skin‐homing. In this patient population, these changes persisted for at least 18 months post‐burn, this 'immune distraction' may limit the ability of immune cells to prioritise other threats post‐burn, such as respiratory infections. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Clinical & Translational Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/cti2.1496 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 1 Subjects: – SubjectFull: MONONUCLEAR leukocytes Type: general – SubjectFull: KILLER cells Type: general – SubjectFull: T cells Type: general – SubjectFull: REGULATORY T cells Type: general – SubjectFull: PEDIATRICS Type: general Titles: – TitleFull: Unremitting pro‐inflammatory T‐cell phenotypes, and macrophage activity, following paediatric burn injury. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Langley, Donna – PersonEntity: Name: NameFull: Zimmermann, Kate – PersonEntity: Name: NameFull: Krenske, Emma – PersonEntity: Name: NameFull: Stefanutti, Giorgio – PersonEntity: Name: NameFull: Kimble, Roy M – PersonEntity: Name: NameFull: Holland, Andrew JA – PersonEntity: Name: NameFull: Fear, Mark W – PersonEntity: Name: NameFull: Wood, Fiona M – PersonEntity: Name: NameFull: Kenna, Tony – PersonEntity: Name: NameFull: Cuttle, Leila IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 20500068 Numbering: – Type: volume Value: 13 – Type: issue Value: 3 Titles: – TitleFull: Clinical & Translational Immunology Type: main |
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