Insight into antioxidant and anti-inflammatory effects of marine bacterial natural exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats.
| Title: | Insight into antioxidant and anti-inflammatory effects of marine bacterial natural exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats. |
|---|---|
| Authors: | El awady, Mohamed E., Mohamed, Sahar S., Abo Elsoud, Mostafa M., Mahmoud, Manal G., Anwar, Mai M., Ahmed, Mahgoub M., Eltaher, Ashraf, Magdeldin, Sameh, Attallah, Ashraf, Elhagry, Ali E., Abdelhamid, Sayeda A. |
| Source: | Scientific Reports; 3/1/2024, Vol. 14 Issue 1, p1-23, 23p |
| Subject Terms: | CARRAGEENANS, MICROBIAL exopolysaccharides, ANALYTICAL chemistry, EDEMA, REACTIVE oxygen species, MARINE sediments, SUPEROXIDE dismutase |
| Geographic Terms: | EGYPT |
| Abstract: | Inflammation is a part of the body's intricate biological reaction to noxious stimuli and defensive reactions. So, the aim of this investigation was to study the anti-inflammatory activity of exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats. A halophilic bacterial strain was isolated from marine sediments in the Red Sea in Egypt. The isolate has been visually and physiologically recognized, as well as by analyzing its 16S rRNA gene, which confirms Kocuria sp. clone Asker4. This particular isolate can be referenced using the accession number OL798051.1. EPSSM was subjected to purification and fractionation by a DEAE-cellulose column. Preliminary chemical analysis of EPSSM indicated that the monosaccharides were fructose, glucuronic acid, and xylose, with 2.0, 0.5, and 1.0, respectively. The antioxidant potential of EPSSM was investigated, and it was discovered that the level of activity increased independently of the concentrations, reaching a maximum threshold of 94.13% at 100 µg/mL of EPSSM for 120 min. Also, EPSSM at 50 mg/kg orally produced a significant anti-inflammatory effect on the carrageenan model at 2, 3, and 4 intervals. The EPSSM intervention resulted in reductions in the levels of catalase and superoxide dismutase enzymes, as well as a decrease in glutathione. Furthermore, the levels of nitric oxide, lipid peroxidation, and reactive oxygen species resulting from carrageenan-induced edema showed a significant reduction subsequent to the administration of EPSSM. Moreover, the findings indicated that the protein expression levels of cyclooxygenase-2 and interleukin-6 were reduced following treatment with EPSSM, resulting in a reduction of paw edema. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Insight into antioxidant and anti-inflammatory effects of marine bacterial natural exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22El+awady%2C+Mohamed+E%2E%22">El awady, Mohamed E.</searchLink><br /><searchLink fieldCode="AR" term="%22Mohamed%2C+Sahar+S%2E%22">Mohamed, Sahar S.</searchLink><br /><searchLink fieldCode="AR" term="%22Abo+Elsoud%2C+Mostafa+M%2E%22">Abo Elsoud, Mostafa M.</searchLink><br /><searchLink fieldCode="AR" term="%22Mahmoud%2C+Manal+G%2E%22">Mahmoud, Manal G.</searchLink><br /><searchLink fieldCode="AR" term="%22Anwar%2C+Mai+M%2E%22">Anwar, Mai M.</searchLink><br /><searchLink fieldCode="AR" term="%22Ahmed%2C+Mahgoub+M%2E%22">Ahmed, Mahgoub M.</searchLink><br /><searchLink fieldCode="AR" term="%22Eltaher%2C+Ashraf%22">Eltaher, Ashraf</searchLink><br /><searchLink fieldCode="AR" term="%22Magdeldin%2C+Sameh%22">Magdeldin, Sameh</searchLink><br /><searchLink fieldCode="AR" term="%22Attallah%2C+Ashraf%22">Attallah, Ashraf</searchLink><br /><searchLink fieldCode="AR" term="%22Elhagry%2C+Ali+E%2E%22">Elhagry, Ali E.</searchLink><br /><searchLink fieldCode="AR" term="%22Abdelhamid%2C+Sayeda+A%2E%22">Abdelhamid, Sayeda A.</searchLink> – Name: TitleSource Label: Source Group: Src Data: Scientific Reports; 3/1/2024, Vol. 14 Issue 1, p1-23, 23p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22CARRAGEENANS%22">CARRAGEENANS</searchLink><br /><searchLink fieldCode="DE" term="%22MICROBIAL+exopolysaccharides%22">MICROBIAL exopolysaccharides</searchLink><br /><searchLink fieldCode="DE" term="%22ANALYTICAL+chemistry%22">ANALYTICAL chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22EDEMA%22">EDEMA</searchLink><br /><searchLink fieldCode="DE" term="%22REACTIVE+oxygen+species%22">REACTIVE oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22MARINE+sediments%22">MARINE sediments</searchLink><br /><searchLink fieldCode="DE" term="%22SUPEROXIDE+dismutase%22">SUPEROXIDE dismutase</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22EGYPT%22">EGYPT</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Inflammation is a part of the body's intricate biological reaction to noxious stimuli and defensive reactions. So, the aim of this investigation was to study the anti-inflammatory activity of exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats. A halophilic bacterial strain was isolated from marine sediments in the Red Sea in Egypt. The isolate has been visually and physiologically recognized, as well as by analyzing its 16S rRNA gene, which confirms Kocuria sp. clone Asker4. This particular isolate can be referenced using the accession number OL798051.1. EPSSM was subjected to purification and fractionation by a DEAE-cellulose column. Preliminary chemical analysis of EPSSM indicated that the monosaccharides were fructose, glucuronic acid, and xylose, with 2.0, 0.5, and 1.0, respectively. The antioxidant potential of EPSSM was investigated, and it was discovered that the level of activity increased independently of the concentrations, reaching a maximum threshold of 94.13% at 100 µg/mL of EPSSM for 120 min. Also, EPSSM at 50 mg/kg orally produced a significant anti-inflammatory effect on the carrageenan model at 2, 3, and 4 intervals. The EPSSM intervention resulted in reductions in the levels of catalase and superoxide dismutase enzymes, as well as a decrease in glutathione. Furthermore, the levels of nitric oxide, lipid peroxidation, and reactive oxygen species resulting from carrageenan-induced edema showed a significant reduction subsequent to the administration of EPSSM. Moreover, the findings indicated that the protein expression levels of cyclooxygenase-2 and interleukin-6 were reduced following treatment with EPSSM, resulting in a reduction of paw edema. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/s41598-024-53502-5 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 23 StartPage: 1 Subjects: – SubjectFull: EGYPT Type: general – SubjectFull: CARRAGEENANS Type: general – SubjectFull: MICROBIAL exopolysaccharides Type: general – SubjectFull: ANALYTICAL chemistry Type: general – SubjectFull: EDEMA Type: general – SubjectFull: REACTIVE oxygen species Type: general – SubjectFull: MARINE sediments Type: general – SubjectFull: SUPEROXIDE dismutase Type: general Titles: – TitleFull: Insight into antioxidant and anti-inflammatory effects of marine bacterial natural exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: El awady, Mohamed E. – PersonEntity: Name: NameFull: Mohamed, Sahar S. – PersonEntity: Name: NameFull: Abo Elsoud, Mostafa M. – PersonEntity: Name: NameFull: Mahmoud, Manal G. – PersonEntity: Name: NameFull: Anwar, Mai M. – PersonEntity: Name: NameFull: Ahmed, Mahgoub M. – PersonEntity: Name: NameFull: Eltaher, Ashraf – PersonEntity: Name: NameFull: Magdeldin, Sameh – PersonEntity: Name: NameFull: Attallah, Ashraf – PersonEntity: Name: NameFull: Elhagry, Ali E. – PersonEntity: Name: NameFull: Abdelhamid, Sayeda A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: 3/1/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 20452322 Numbering: – Type: volume Value: 14 – Type: issue Value: 1 Titles: – TitleFull: Scientific Reports Type: main |
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