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Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout.

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Title: Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout.
Authors: Xiong, Lei, Liu, Zhaoxia, Li, Jingge, Yao, Shuyuan, Li, Zeyun, Chen, Xuanhao, Shen, Lingling, Zhang, Zhen, Li, Yongbin, Hou, Qing, Zhang, Yuhang, You, Minsheng, Yuchi, Zhiguang, You, Shijun
Source: Toxins; Apr2023, Vol. 15 Issue 4, p273, 17p
Subject Terms: DIAMONDBACK moth, ATP-binding cassette transporters, CRISPRS, BACILLUS thuringiensis, INSECTICIDE resistance
Abstract: Many insects, including the Plutella xylostella (L.), have developed varying degrees of resistance to many insecticides, including Bacillus thuringiensis (Bt) toxins, the bioinsecticides derived from Bt. The polycalin protein is one of the potential receptors for Bt toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of P. xylostella, but whether polycalin is associated with the resistance of Bt toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the Pxpolycalin gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of Pxpolycalin showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the Pxpolycalin gene and its transcript level were not linked to Cry1Ac resistance, whereas both the PxABCC2 gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the Pxpolycalin gene in a short term. Furthermore, the knockout of polycalin and ATP-binding cassette transporter subfamily C2 (ABCC2) genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to Bt toxins. [ABSTRACT FROM AUTHOR]
Copyright of Toxins is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Analysis of the Effect of Plutella xylostella Polycalin and ABCC2 Transporter on Cry1Ac Susceptibility by CRISPR/Cas9-Mediated Knockout.
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  Data: <searchLink fieldCode="AR" term="%22Xiong%2C+Lei%22">Xiong, Lei</searchLink><br /><searchLink fieldCode="AR" term="%22Liu%2C+Zhaoxia%22">Liu, Zhaoxia</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Jingge%22">Li, Jingge</searchLink><br /><searchLink fieldCode="AR" term="%22Yao%2C+Shuyuan%22">Yao, Shuyuan</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Zeyun%22">Li, Zeyun</searchLink><br /><searchLink fieldCode="AR" term="%22Chen%2C+Xuanhao%22">Chen, Xuanhao</searchLink><br /><searchLink fieldCode="AR" term="%22Shen%2C+Lingling%22">Shen, Lingling</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Zhen%22">Zhang, Zhen</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Yongbin%22">Li, Yongbin</searchLink><br /><searchLink fieldCode="AR" term="%22Hou%2C+Qing%22">Hou, Qing</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yuhang%22">Zhang, Yuhang</searchLink><br /><searchLink fieldCode="AR" term="%22You%2C+Minsheng%22">You, Minsheng</searchLink><br /><searchLink fieldCode="AR" term="%22Yuchi%2C+Zhiguang%22">Yuchi, Zhiguang</searchLink><br /><searchLink fieldCode="AR" term="%22You%2C+Shijun%22">You, Shijun</searchLink>
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  Data: Toxins; Apr2023, Vol. 15 Issue 4, p273, 17p
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  Data: <searchLink fieldCode="DE" term="%22DIAMONDBACK+moth%22">DIAMONDBACK moth</searchLink><br /><searchLink fieldCode="DE" term="%22ATP-binding+cassette+transporters%22">ATP-binding cassette transporters</searchLink><br /><searchLink fieldCode="DE" term="%22CRISPRS%22">CRISPRS</searchLink><br /><searchLink fieldCode="DE" term="%22BACILLUS+thuringiensis%22">BACILLUS thuringiensis</searchLink><br /><searchLink fieldCode="DE" term="%22INSECTICIDE+resistance%22">INSECTICIDE resistance</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Many insects, including the Plutella xylostella (L.), have developed varying degrees of resistance to many insecticides, including Bacillus thuringiensis (Bt) toxins, the bioinsecticides derived from Bt. The polycalin protein is one of the potential receptors for Bt toxins, and previous studies have confirmed that the Cry1Ac toxin can bind to the polycalin protein of P. xylostella, but whether polycalin is associated with the resistance of Bt toxins remains controversial. In this study, we compared the midgut of larvae from Cry1Ac-susceptible and -resistant strains, and found that the expression of the Pxpolycalin gene was largely reduced in the midgut of the resistant strains. Moreover, the spatial and temporal expression patterns of Pxpolycalin showed that it was mainly expressed in the larval stage and midgut tissue. However, genetic linkage experiments showed that the Pxpolycalin gene and its transcript level were not linked to Cry1Ac resistance, whereas both the PxABCC2 gene and its transcript levels were linked to Cry1Ac resistance. The larvae fed on a diet containing the Cry1Ac toxin showed no significant change in the expression of the Pxpolycalin gene in a short term. Furthermore, the knockout of polycalin and ATP-binding cassette transporter subfamily C2 (ABCC2) genes separately by CRISPR/Cas9 technology resulted in resistance to decreased susceptibility to Cry1Ac toxin. Our results provide new insights into the potential role of polycalin and ABCC2 proteins in Cry1Ac resistance and the mechanism underlying the resistance of insects to Bt toxins. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Toxins is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/toxins15040273
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        Text: English
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              Text: Apr2023
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