A novel 17 bp indel in the SMAD3 gene alters transcription level, contributing to phenotypic traits in Chinese cattle.
| Title: | A novel 17 bp indel in the SMAD3 gene alters transcription level, contributing to phenotypic traits in Chinese cattle. |
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| Authors: | Tao Shi, Wenwen Peng, Jianyu Yan, Hanfang Cai, Xianyong Lan, Chuzhao Lei, Yueyu Bai, Hong Chen |
| Source: | Archives Animal Breeding / Archiv Tierzucht; 2016, Vol. 59 Issue 1, p151-157, 7p, 4 Charts, 4 Graphs |
| Abstract: | SMAD3, the messenger of the transforming growth factor beta (TGF-β) signaling pathway, plays essential roles in myogenesis and osteogenesis and may relate to the regulation of body weight. In this study, a 17 bp indel (NC_007308: g.101893_101909insGAGGATGAGTGCTCCAG) in intron3 of the SMAD3 gene was detected in four Chinese cattle breeds (Qinchuan, Jiaxian, Nanyang and Caoyuan) by using DNA pool sequencing, and its effects on gene expression and growth traits were analyzed in Qinchuan and Caoyuan cattle. The results showed that the indel locus was significantly associated with SMAD3 transcriptional levels where II genotypes had a higher value than DD genotypes in Qinchuan (QC) cattle muscle tissue (P <0.05). In addition, the locus was strongly associated with chest girth, chest width, rump length, hucklebone width and body weight in 2-year-old QC cattle (P <0.05) and body weight (12 months), body height (18 months) and chest girth (18 months) in Caoyuan cattle (P <0.5). To the best of our knowledge, this is the first evidence of the association between SMAD3 indel and cattle phenotype, and it may contribute to understanding the function of the indel, which could be a promising marker for beef cattle breeding. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: A novel 17 bp indel in the SMAD3 gene alters transcription level, contributing to phenotypic traits in Chinese cattle. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tao+Shi%22">Tao Shi</searchLink><br /><searchLink fieldCode="AR" term="%22Wenwen+Peng%22">Wenwen Peng</searchLink><br /><searchLink fieldCode="AR" term="%22Jianyu+Yan%22">Jianyu Yan</searchLink><br /><searchLink fieldCode="AR" term="%22Hanfang+Cai%22">Hanfang Cai</searchLink><br /><searchLink fieldCode="AR" term="%22Xianyong+Lan%22">Xianyong Lan</searchLink><br /><searchLink fieldCode="AR" term="%22Chuzhao+Lei%22">Chuzhao Lei</searchLink><br /><searchLink fieldCode="AR" term="%22Yueyu+Bai%22">Yueyu Bai</searchLink><br /><searchLink fieldCode="AR" term="%22Hong+Chen%22">Hong Chen</searchLink> – Name: TitleSource Label: Source Group: Src Data: Archives Animal Breeding / Archiv Tierzucht; 2016, Vol. 59 Issue 1, p151-157, 7p, 4 Charts, 4 Graphs – Name: Abstract Label: Abstract Group: Ab Data: SMAD3, the messenger of the transforming growth factor beta (TGF-β) signaling pathway, plays essential roles in myogenesis and osteogenesis and may relate to the regulation of body weight. In this study, a 17 bp indel (NC_007308: g.101893_101909insGAGGATGAGTGCTCCAG) in intron3 of the SMAD3 gene was detected in four Chinese cattle breeds (Qinchuan, Jiaxian, Nanyang and Caoyuan) by using DNA pool sequencing, and its effects on gene expression and growth traits were analyzed in Qinchuan and Caoyuan cattle. The results showed that the indel locus was significantly associated with SMAD3 transcriptional levels where II genotypes had a higher value than DD genotypes in Qinchuan (QC) cattle muscle tissue (P <0.05). In addition, the locus was strongly associated with chest girth, chest width, rump length, hucklebone width and body weight in 2-year-old QC cattle (P <0.05) and body weight (12 months), body height (18 months) and chest girth (18 months) in Caoyuan cattle (P <0.5). To the best of our knowledge, this is the first evidence of the association between SMAD3 indel and cattle phenotype, and it may contribute to understanding the function of the indel, which could be a promising marker for beef cattle breeding. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Archives Animal Breeding / Archiv Tierzucht is the property of Copernicus Gesellschaft mbH and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.5194/aab-59-151-2016 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 151 Titles: – TitleFull: A novel 17 bp indel in the SMAD3 gene alters transcription level, contributing to phenotypic traits in Chinese cattle. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tao Shi – PersonEntity: Name: NameFull: Wenwen Peng – PersonEntity: Name: NameFull: Jianyu Yan – PersonEntity: Name: NameFull: Hanfang Cai – PersonEntity: Name: NameFull: Xianyong Lan – PersonEntity: Name: NameFull: Chuzhao Lei – PersonEntity: Name: NameFull: Yueyu Bai – PersonEntity: Name: NameFull: Hong Chen IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: 2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 00039438 Numbering: – Type: volume Value: 59 – Type: issue Value: 1 Titles: – TitleFull: Archives Animal Breeding / Archiv Tierzucht Type: main |
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