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Long-term organoid culture of a small intestinal neuroendocrine tumor.

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Title: Long-term organoid culture of a small intestinal neuroendocrine tumor.
Authors: D’Agosto, Sabrina, Fiorini, Elena, Pezzini, Francesco, Delfino, Pietro, Simbolo, Michele, Vicentini, Caterina, Andreani, Silvia, Capelli, Paola, Rusev, Borislav, Lawlor, Rita T., Bassi, Claudio, Landoni, Luca, Pea, Antonio, Luchini, Claudio, Scarpa, Aldo, Corbo, Vincenzo
Source: Frontiers in Endocrinology; 4/4/2023, Vol. 14, p1-12, 12p
Subject Terms: NEUROENDOCRINE tumors, INTESTINAL tumors, CANCER cell culture, HUMAN cell culture, GENE expression
Abstract: Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are rare and highly heterogeneous neoplasms whose incidence has markedly increased over the last decades. A grading system based on the tumor cells’ proliferation index predicts high-risk for G3 NETs. However, low-to-intermediate grade (G1/G2) NETs have an unpredictable clinical course that varies from indolent to highly malignant. Cultures of human cancer cells enable to perform functional perturbation analyses that are instrumental to enhance our understanding of cancer biology. To date, no tractable and reliable long-term culture of G1/G2 NET has been reported to permit disease modeling and pharmacological screens. Here, we report of the first long-term culture of a G2 metastatic small intestinal NET that preserves the main genetic drivers of the tumor and retains expression patterns of the endocrine cell lineage. Replicating the tissue, this long-term culture showed a low proliferation index, and yet it could be propagated continuously without dramatic changes in the karyotype. The model was readily available for pharmacological screens using targeted agents and as expected, showed low tumorigenic capacity in vivo. Overall, this is the first long-term culture of NETs to faithfully recapitulate many aspects of the original neuroendocrine tumor. [ABSTRACT FROM AUTHOR]
Copyright of Frontiers in Endocrinology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Long-term organoid culture of a small intestinal neuroendocrine tumor.
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  Data: Frontiers in Endocrinology; 4/4/2023, Vol. 14, p1-12, 12p
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  Data: <searchLink fieldCode="DE" term="%22NEUROENDOCRINE+tumors%22">NEUROENDOCRINE tumors</searchLink><br /><searchLink fieldCode="DE" term="%22INTESTINAL+tumors%22">INTESTINAL tumors</searchLink><br /><searchLink fieldCode="DE" term="%22CANCER+cell+culture%22">CANCER cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22HUMAN+cell+culture%22">HUMAN cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink>
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  Label: Abstract
  Group: Ab
  Data: Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are rare and highly heterogeneous neoplasms whose incidence has markedly increased over the last decades. A grading system based on the tumor cells’ proliferation index predicts high-risk for G3 NETs. However, low-to-intermediate grade (G1/G2) NETs have an unpredictable clinical course that varies from indolent to highly malignant. Cultures of human cancer cells enable to perform functional perturbation analyses that are instrumental to enhance our understanding of cancer biology. To date, no tractable and reliable long-term culture of G1/G2 NET has been reported to permit disease modeling and pharmacological screens. Here, we report of the first long-term culture of a G2 metastatic small intestinal NET that preserves the main genetic drivers of the tumor and retains expression patterns of the endocrine cell lineage. Replicating the tissue, this long-term culture showed a low proliferation index, and yet it could be propagated continuously without dramatic changes in the karyotype. The model was readily available for pharmacological screens using targeted agents and as expected, showed low tumorigenic capacity in vivo. Overall, this is the first long-term culture of NETs to faithfully recapitulate many aspects of the original neuroendocrine tumor. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Frontiers in Endocrinology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3389/fendo.2023.999792
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      – Code: eng
        Text: English
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        PageCount: 12
        StartPage: 1
    Subjects:
      – SubjectFull: NEUROENDOCRINE tumors
        Type: general
      – SubjectFull: INTESTINAL tumors
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      – SubjectFull: CANCER cell culture
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      – SubjectFull: HUMAN cell culture
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      – SubjectFull: GENE expression
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              Text: 4/4/2023
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