Differential expression of immune-regulatory proteins C5AR1, CLEC4A and NLRP3 on peripheral blood mononuclear cells in early-stage non-small cell lung cancer patients.
| Title: | Differential expression of immune-regulatory proteins C5AR1, CLEC4A and NLRP3 on peripheral blood mononuclear cells in early-stage non-small cell lung cancer patients. |
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| Authors: | Pakvisal, Nussara, Kongkavitoon, Pornrat, Sathitruangsak, Chirawadee, Pornpattanarak, Nopporn, Boonsirikamchai, Piyaporn, Ouwongprayoon, Pongsakorn, Aporntewan, Chatchawit, Chantranuwatana, Poonchavist, Mutirangura, Apiwat, Vinayanuwattikun, Chanida |
| Source: | Scientific Reports; 11/2/2022, Vol. 12 Issue 1, p1-9, 9p |
| Subject Terms: | MONONUCLEAR leukocytes, NON-small-cell lung carcinoma, CANCER patients, GENE expression profiling, PROTEIN expression |
| Abstract: | Changes in gene expression profiling of peripheral blood mononuclear cells (PBMC) appear to represent the host's response to the cancer cells via paracrine signaling. We speculated that protein expression on circulating T-lymphocytes represent T-lymphocyte trafficking before infiltration into the tumor microenvironment. The possibility of using protein expression on circulating T-lymphocytes as a biomarker to discriminate early-stage non-small cell lung cancer (NSCLC) was explored. Four independent PBMC gene expression microarray datasets (GSE12771, GSE13255, GSE20189 and GSE3934) were analyzed. We selected C5AR1, CLEC4A and NLRP3 based on their significant protein expression in tumor-infiltrating lymphocytes, but not in normal lymphoid tissue. A validation study using automated flow cytometry was conducted in 141 study participants including 76 treatment-naive early-stage non-small cell lung cancer patients (NSCLC), 12 individuals with non-malignant pulmonary diseases, and 53 healthy individuals. Median ratios of C5AR1, CLEC4A and NLRP3 specific antibody staining to CD3 positive cells in early-stage NSCLC patients compared to healthy controls were 0.014 [0–0.37] vs. 0.01 [0–0.07, p = 0.13], 0.03 [0–0.87] vs. 0.02 [0–0.13, p = 0.10] and 0.19 [0–0.60] vs. 0.09 [0.02–0.31, p < 0.0001], respectively. Median fluorescence intensity (MFI) of CD3+C5AR1+, CD3+CLEC4A+ and CD3+NLRP3+ expression in early-stage NSCLC patients compared to healthy volunteers was 185 [64.2–4801] vs. 107.5 [27–229, p < 0.0001], 91.2 [42.4–2355] vs. 71.25 [46.2–103, p = 0.0005], and 1585 [478–5224] vs. 758.5 [318–1976, p < 0.0001], respectively. NLRP3:CD3 ratio, CD3+C5AR1+, CD3+CLEC4A+ and CD3+NLRP3+ MFI were significantly higher in early-stage NSCLC than healthy volunteers with an area under the ROC curve of 0.69–0.76. The CD3+NLRP3+ MFI provided the most distinguishable expression at 71.5% sensitivity and 70% specificity. Furthermore, CD3+NLRP3+ MFI potentially discriminated between early-stage NSCLC from malignant-mimic inflammation and infection pulmonary disease. Further validation in various pulmonary inflammatory disease might be warranted. Our proof-of-principle findings strengthen the hypothesis that malignancies generate distinctive protein expression fingerprints on circulating T-lymphocytes. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Differential expression of immune-regulatory proteins C5AR1, CLEC4A and NLRP3 on peripheral blood mononuclear cells in early-stage non-small cell lung cancer patients. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Pakvisal%2C+Nussara%22">Pakvisal, Nussara</searchLink><br /><searchLink fieldCode="AR" term="%22Kongkavitoon%2C+Pornrat%22">Kongkavitoon, Pornrat</searchLink><br /><searchLink fieldCode="AR" term="%22Sathitruangsak%2C+Chirawadee%22">Sathitruangsak, Chirawadee</searchLink><br /><searchLink fieldCode="AR" term="%22Pornpattanarak%2C+Nopporn%22">Pornpattanarak, Nopporn</searchLink><br /><searchLink fieldCode="AR" term="%22Boonsirikamchai%2C+Piyaporn%22">Boonsirikamchai, Piyaporn</searchLink><br /><searchLink fieldCode="AR" term="%22Ouwongprayoon%2C+Pongsakorn%22">Ouwongprayoon, Pongsakorn</searchLink><br /><searchLink fieldCode="AR" term="%22Aporntewan%2C+Chatchawit%22">Aporntewan, Chatchawit</searchLink><br /><searchLink fieldCode="AR" term="%22Chantranuwatana%2C+Poonchavist%22">Chantranuwatana, Poonchavist</searchLink><br /><searchLink fieldCode="AR" term="%22Mutirangura%2C+Apiwat%22">Mutirangura, Apiwat</searchLink><br /><searchLink fieldCode="AR" term="%22Vinayanuwattikun%2C+Chanida%22">Vinayanuwattikun, Chanida</searchLink> – Name: TitleSource Label: Source Group: Src Data: Scientific Reports; 11/2/2022, Vol. 12 Issue 1, p1-9, 9p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22MONONUCLEAR+leukocytes%22">MONONUCLEAR leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22NON-small-cell+lung+carcinoma%22">NON-small-cell lung carcinoma</searchLink><br /><searchLink fieldCode="DE" term="%22CANCER+patients%22">CANCER patients</searchLink><br /><searchLink fieldCode="DE" term="%22GENE+expression+profiling%22">GENE expression profiling</searchLink><br /><searchLink fieldCode="DE" term="%22PROTEIN+expression%22">PROTEIN expression</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Changes in gene expression profiling of peripheral blood mononuclear cells (PBMC) appear to represent the host's response to the cancer cells via paracrine signaling. We speculated that protein expression on circulating T-lymphocytes represent T-lymphocyte trafficking before infiltration into the tumor microenvironment. The possibility of using protein expression on circulating T-lymphocytes as a biomarker to discriminate early-stage non-small cell lung cancer (NSCLC) was explored. Four independent PBMC gene expression microarray datasets (GSE12771, GSE13255, GSE20189 and GSE3934) were analyzed. We selected C5AR1, CLEC4A and NLRP3 based on their significant protein expression in tumor-infiltrating lymphocytes, but not in normal lymphoid tissue. A validation study using automated flow cytometry was conducted in 141 study participants including 76 treatment-naive early-stage non-small cell lung cancer patients (NSCLC), 12 individuals with non-malignant pulmonary diseases, and 53 healthy individuals. Median ratios of C5AR1, CLEC4A and NLRP3 specific antibody staining to CD3 positive cells in early-stage NSCLC patients compared to healthy controls were 0.014 [0–0.37] vs. 0.01 [0–0.07, p = 0.13], 0.03 [0–0.87] vs. 0.02 [0–0.13, p = 0.10] and 0.19 [0–0.60] vs. 0.09 [0.02–0.31, p < 0.0001], respectively. Median fluorescence intensity (MFI) of CD3<superscript>+</superscript>C5AR1<superscript>+</superscript>, CD3<superscript>+</superscript>CLEC4A<superscript>+</superscript> and CD3<superscript>+</superscript>NLRP3<superscript>+</superscript> expression in early-stage NSCLC patients compared to healthy volunteers was 185 [64.2–4801] vs. 107.5 [27–229, p < 0.0001], 91.2 [42.4–2355] vs. 71.25 [46.2–103, p = 0.0005], and 1585 [478–5224] vs. 758.5 [318–1976, p < 0.0001], respectively. NLRP3:CD3 ratio, CD3<superscript>+</superscript>C5AR1<superscript>+</superscript>, CD3<superscript>+</superscript>CLEC4A<superscript>+</superscript> and CD3<superscript>+</superscript>NLRP3<superscript>+</superscript> MFI were significantly higher in early-stage NSCLC than healthy volunteers with an area under the ROC curve of 0.69–0.76. The CD3<superscript>+</superscript>NLRP3<superscript>+</superscript> MFI provided the most distinguishable expression at 71.5% sensitivity and 70% specificity. Furthermore, CD3<superscript>+</superscript>NLRP3<superscript>+</superscript> MFI potentially discriminated between early-stage NSCLC from malignant-mimic inflammation and infection pulmonary disease. Further validation in various pulmonary inflammatory disease might be warranted. Our proof-of-principle findings strengthen the hypothesis that malignancies generate distinctive protein expression fingerprints on circulating T-lymphocytes. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/s41598-022-21891-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: MONONUCLEAR leukocytes Type: general – SubjectFull: NON-small-cell lung carcinoma Type: general – SubjectFull: CANCER patients Type: general – SubjectFull: GENE expression profiling Type: general – SubjectFull: PROTEIN expression Type: general Titles: – TitleFull: Differential expression of immune-regulatory proteins C5AR1, CLEC4A and NLRP3 on peripheral blood mononuclear cells in early-stage non-small cell lung cancer patients. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Pakvisal, Nussara – PersonEntity: Name: NameFull: Kongkavitoon, Pornrat – PersonEntity: Name: NameFull: Sathitruangsak, Chirawadee – PersonEntity: Name: NameFull: Pornpattanarak, Nopporn – PersonEntity: Name: NameFull: Boonsirikamchai, Piyaporn – PersonEntity: Name: NameFull: Ouwongprayoon, Pongsakorn – PersonEntity: Name: NameFull: Aporntewan, Chatchawit – PersonEntity: Name: NameFull: Chantranuwatana, Poonchavist – PersonEntity: Name: NameFull: Mutirangura, Apiwat – PersonEntity: Name: NameFull: Vinayanuwattikun, Chanida IsPartOfRelationships: – BibEntity: Dates: – D: 02 M: 11 Text: 11/2/2022 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 20452322 Numbering: – Type: volume Value: 12 – Type: issue Value: 1 Titles: – TitleFull: Scientific Reports Type: main |
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