Evaluation of Three Anti-SARS-CoV-2 Serologic Immunoassays for Post-Vaccine Response.
| Title: | Evaluation of Three Anti-SARS-CoV-2 Serologic Immunoassays for Post-Vaccine Response. |
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| Authors: | Di Meo, Ashley, Miller, Jessica J, Fabros, Anselmo, Brinc, Davor, Hall, Victoria, Pinzon, Natalia, Ierullo, Matthew, Ku, Terrance, Ferreira, Victor H, Kumar, Deepali, Pasic, Maria D, Kulasingam, Vathany |
| Source: | Journal of Applied Laboratory Medicine; Jan2022, Vol. 7 Issue 1, p57-65, 9p |
| Subject Terms: | SARS-CoV-2, IMMUNOASSAY, IMMUNOGLOBULINS, FETOFETAL transfusion, MEDICAL personnel |
| Geographic Terms: | NORTH America |
| Abstract: | Background: In North America, both messenger RNA (mRNA) vaccines, Pfizer-BioNTech BNT162b2, and Moderna mRNA-1273, each utilizing a 2-dose regimen, have started to be administered to individuals. Methods: We evaluated the quantitative serologic antibody response following administration of either a single dose or both doses of an mRNA severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in a cohort of 98 participants (88 healthcare workers [HCW] and 10 solid organ transplant [SOT] recipients). Antibody levels were compared across 3 immunoassays: Elecsys Anti-SARS-CoV-2 S (Roche Diagnostics), SARS-CoV-2 TrimericS IgG (DiaSorin), and SARS-CoV-2 IgG II Quant (Abbott). Results: Among HCW, sensitivity ranged from 100% (Roche), 99% (Abbott) and 98% (DiaSorin). The SARS-CoV-2 IgG II Quant and SARS-CoV-2 TrimericS IgG assays showed good agreement with a Pearson correlation coefficient of R = 0.95. Pearson correlation coefficients of R = 0.82 and 0.83 were obtained for Elecsys Anti-SARS-CoV-2 S vs SARS-CoV-2 TrimericS IgG and SARS-CoV-2 IgG II Quant vs Elecsys Anti-SARS-CoV-2 S, respectively. Significant differences in antibody levels between HCW and SOT recipients were observed. A decrease in antibody levels from time of vaccine administration to blood draw was evident. Among those with a second dose, an increase in antibody levels with increased time between administration of the first and second dose was observed. Conclusions: The absolute values generated from each of the assay platforms are not interchangeable. Antibody levels differed with increased time between vaccine administration and with increased time between administration of the first and second dose. Further, significant differences in antibody levels between HCW and SOT recipients were observed. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Evaluation of Three Anti-SARS-CoV-2 Serologic Immunoassays for Post-Vaccine Response. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Di+Meo%2C+Ashley%22">Di Meo, Ashley</searchLink><br /><searchLink fieldCode="AR" term="%22Miller%2C+Jessica+J%22">Miller, Jessica J</searchLink><br /><searchLink fieldCode="AR" term="%22Fabros%2C+Anselmo%22">Fabros, Anselmo</searchLink><br /><searchLink fieldCode="AR" term="%22Brinc%2C+Davor%22">Brinc, Davor</searchLink><br /><searchLink fieldCode="AR" term="%22Hall%2C+Victoria%22">Hall, Victoria</searchLink><br /><searchLink fieldCode="AR" term="%22Pinzon%2C+Natalia%22">Pinzon, Natalia</searchLink><br /><searchLink fieldCode="AR" term="%22Ierullo%2C+Matthew%22">Ierullo, Matthew</searchLink><br /><searchLink fieldCode="AR" term="%22Ku%2C+Terrance%22">Ku, Terrance</searchLink><br /><searchLink fieldCode="AR" term="%22Ferreira%2C+Victor+H%22">Ferreira, Victor H</searchLink><br /><searchLink fieldCode="AR" term="%22Kumar%2C+Deepali%22">Kumar, Deepali</searchLink><br /><searchLink fieldCode="AR" term="%22Pasic%2C+Maria+D%22">Pasic, Maria D</searchLink><br /><searchLink fieldCode="AR" term="%22Kulasingam%2C+Vathany%22">Kulasingam, Vathany</searchLink> – Name: TitleSource Label: Source Group: Src Data: Journal of Applied Laboratory Medicine; Jan2022, Vol. 7 Issue 1, p57-65, 9p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22SARS-CoV-2%22">SARS-CoV-2</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNOASSAY%22">IMMUNOASSAY</searchLink><br /><searchLink fieldCode="DE" term="%22IMMUNOGLOBULINS%22">IMMUNOGLOBULINS</searchLink><br /><searchLink fieldCode="DE" term="%22FETOFETAL+transfusion%22">FETOFETAL transfusion</searchLink><br /><searchLink fieldCode="DE" term="%22MEDICAL+personnel%22">MEDICAL personnel</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22NORTH+America%22">NORTH America</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background: In North America, both messenger RNA (mRNA) vaccines, Pfizer-BioNTech BNT162b2, and Moderna mRNA-1273, each utilizing a 2-dose regimen, have started to be administered to individuals. Methods: We evaluated the quantitative serologic antibody response following administration of either a single dose or both doses of an mRNA severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in a cohort of 98 participants (88 healthcare workers [HCW] and 10 solid organ transplant [SOT] recipients). Antibody levels were compared across 3 immunoassays: Elecsys Anti-SARS-CoV-2 S (Roche Diagnostics), SARS-CoV-2 TrimericS IgG (DiaSorin), and SARS-CoV-2 IgG II Quant (Abbott). Results: Among HCW, sensitivity ranged from 100% (Roche), 99% (Abbott) and 98% (DiaSorin). The SARS-CoV-2 IgG II Quant and SARS-CoV-2 TrimericS IgG assays showed good agreement with a Pearson correlation coefficient of R = 0.95. Pearson correlation coefficients of R = 0.82 and 0.83 were obtained for Elecsys Anti-SARS-CoV-2 S vs SARS-CoV-2 TrimericS IgG and SARS-CoV-2 IgG II Quant vs Elecsys Anti-SARS-CoV-2 S, respectively. Significant differences in antibody levels between HCW and SOT recipients were observed. A decrease in antibody levels from time of vaccine administration to blood draw was evident. Among those with a second dose, an increase in antibody levels with increased time between administration of the first and second dose was observed. Conclusions: The absolute values generated from each of the assay platforms are not interchangeable. Antibody levels differed with increased time between vaccine administration and with increased time between administration of the first and second dose. Further, significant differences in antibody levels between HCW and SOT recipients were observed. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Journal of Applied Laboratory Medicine is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1093/jalm/jfab087 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 57 Subjects: – SubjectFull: NORTH America Type: general – SubjectFull: SARS-CoV-2 Type: general – SubjectFull: IMMUNOASSAY Type: general – SubjectFull: IMMUNOGLOBULINS Type: general – SubjectFull: FETOFETAL transfusion Type: general – SubjectFull: MEDICAL personnel Type: general Titles: – TitleFull: Evaluation of Three Anti-SARS-CoV-2 Serologic Immunoassays for Post-Vaccine Response. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Di Meo, Ashley – PersonEntity: Name: NameFull: Miller, Jessica J – PersonEntity: Name: NameFull: Fabros, Anselmo – PersonEntity: Name: NameFull: Brinc, Davor – PersonEntity: Name: NameFull: Hall, Victoria – PersonEntity: Name: NameFull: Pinzon, Natalia – PersonEntity: Name: NameFull: Ierullo, Matthew – PersonEntity: Name: NameFull: Ku, Terrance – PersonEntity: Name: NameFull: Ferreira, Victor H – PersonEntity: Name: NameFull: Kumar, Deepali – PersonEntity: Name: NameFull: Pasic, Maria D – PersonEntity: Name: NameFull: Kulasingam, Vathany IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2022 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 24757241 Numbering: – Type: volume Value: 7 – Type: issue Value: 1 Titles: – TitleFull: Journal of Applied Laboratory Medicine Type: main |
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