Integrative RNA-Seq and ATAC-Seq Analysis Reveals the Migration-Associated Genes Involved in Antitumor Effects of Herbal Medicine Feiyanning on Lung Cancer Cells.
| Title: | Integrative RNA-Seq and ATAC-Seq Analysis Reveals the Migration-Associated Genes Involved in Antitumor Effects of Herbal Medicine Feiyanning on Lung Cancer Cells. |
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| Authors: | Wang, Chenyang, Li, Pengxiao, Peng, Yonglin, Liu, Ruiqi, Wu, Xiaoting, Tan, Sheng, Zhang, Ming, Zhao, Xiaodong |
| Source: | Frontiers in Genetics; 12/21/2021, Vol. 12, p1-8, 8p |
| Subject Terms: | LUNG cancer, NON-small-cell lung carcinoma, CANCER cells, HERBAL medicine, LUNGS, CHINESE medicine, CELL anatomy |
| Abstract: | Lung cancer is one of the leading causes of cancer-associated death in the world. It is of great importance to explore new therapeutic targets. Traditional Chinese medicine formula Feiyanning has been clinically administered in China for more than a decade and raised attention due to its anticancer effect in lung cancer. However, the underlying molecular mechanisms remain to be elucidated. In the present study, we carried out cellular and molecular assays to examine the antitumor activities and understand the mechanism of the Feiyanning formula in lung cancer cells. The cellular viability of Feiyanning-treated lung cancer cells was evaluated by Cell Counting Kit-8. The effect of the Feiyanning formula on cellular migration and invasion of lung cancer cells was examined by wound healing and transwell assays. Transcriptome and chromatin accessibility analysis by RNA-seq and ATAC-seq was performed to investigate the underlying molecular mechanisms. Our results revealed that the Feiyanning formula inhibited the cellular activities of proliferation, migration, and invasion in non-small cell lung cancer cell lines A549, H1975, and 95D. Furthermore, we observed that the transcriptional activity of the migration-associated genes was downregulated upon Feiyanning formula treatment in non-small cell lung cancer cells. The chromatin accessibility of the Feiyanning-treated lung cancer genome tended to decrease, and the regulation of the cellular component movement biological process and PI3K-AKT pathway were enriched among these altered genomic regions. Taken together, the present study suggested that Feiyanning formula exerted the antitumor effects by modulating the expression and chromatin accessibility levels of migration-associated genes. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Integrative RNA-Seq and ATAC-Seq Analysis Reveals the Migration-Associated Genes Involved in Antitumor Effects of Herbal Medicine Feiyanning on Lung Cancer Cells. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Chenyang%22">Wang, Chenyang</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Pengxiao%22">Li, Pengxiao</searchLink><br /><searchLink fieldCode="AR" term="%22Peng%2C+Yonglin%22">Peng, Yonglin</searchLink><br /><searchLink fieldCode="AR" term="%22Liu%2C+Ruiqi%22">Liu, Ruiqi</searchLink><br /><searchLink fieldCode="AR" term="%22Wu%2C+Xiaoting%22">Wu, Xiaoting</searchLink><br /><searchLink fieldCode="AR" term="%22Tan%2C+Sheng%22">Tan, Sheng</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Ming%22">Zhang, Ming</searchLink><br /><searchLink fieldCode="AR" term="%22Zhao%2C+Xiaodong%22">Zhao, Xiaodong</searchLink> – Name: TitleSource Label: Source Group: Src Data: Frontiers in Genetics; 12/21/2021, Vol. 12, p1-8, 8p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22LUNG+cancer%22">LUNG cancer</searchLink><br /><searchLink fieldCode="DE" term="%22NON-small-cell+lung+carcinoma%22">NON-small-cell lung carcinoma</searchLink><br /><searchLink fieldCode="DE" term="%22CANCER+cells%22">CANCER cells</searchLink><br /><searchLink fieldCode="DE" term="%22HERBAL+medicine%22">HERBAL medicine</searchLink><br /><searchLink fieldCode="DE" term="%22LUNGS%22">LUNGS</searchLink><br /><searchLink fieldCode="DE" term="%22CHINESE+medicine%22">CHINESE medicine</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+anatomy%22">CELL anatomy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Lung cancer is one of the leading causes of cancer-associated death in the world. It is of great importance to explore new therapeutic targets. Traditional Chinese medicine formula Feiyanning has been clinically administered in China for more than a decade and raised attention due to its anticancer effect in lung cancer. However, the underlying molecular mechanisms remain to be elucidated. In the present study, we carried out cellular and molecular assays to examine the antitumor activities and understand the mechanism of the Feiyanning formula in lung cancer cells. The cellular viability of Feiyanning-treated lung cancer cells was evaluated by Cell Counting Kit-8. The effect of the Feiyanning formula on cellular migration and invasion of lung cancer cells was examined by wound healing and transwell assays. Transcriptome and chromatin accessibility analysis by RNA-seq and ATAC-seq was performed to investigate the underlying molecular mechanisms. Our results revealed that the Feiyanning formula inhibited the cellular activities of proliferation, migration, and invasion in non-small cell lung cancer cell lines A549, H1975, and 95D. Furthermore, we observed that the transcriptional activity of the migration-associated genes was downregulated upon Feiyanning formula treatment in non-small cell lung cancer cells. The chromatin accessibility of the Feiyanning-treated lung cancer genome tended to decrease, and the regulation of the cellular component movement biological process and PI3K-AKT pathway were enriched among these altered genomic regions. Taken together, the present study suggested that Feiyanning formula exerted the antitumor effects by modulating the expression and chromatin accessibility levels of migration-associated genes. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Frontiers in Genetics is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3389/fgene.2021.799099 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 1 Subjects: – SubjectFull: LUNG cancer Type: general – SubjectFull: NON-small-cell lung carcinoma Type: general – SubjectFull: CANCER cells Type: general – SubjectFull: HERBAL medicine Type: general – SubjectFull: LUNGS Type: general – SubjectFull: CHINESE medicine Type: general – SubjectFull: CELL anatomy Type: general Titles: – TitleFull: Integrative RNA-Seq and ATAC-Seq Analysis Reveals the Migration-Associated Genes Involved in Antitumor Effects of Herbal Medicine Feiyanning on Lung Cancer Cells. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Chenyang – PersonEntity: Name: NameFull: Li, Pengxiao – PersonEntity: Name: NameFull: Peng, Yonglin – PersonEntity: Name: NameFull: Liu, Ruiqi – PersonEntity: Name: NameFull: Wu, Xiaoting – PersonEntity: Name: NameFull: Tan, Sheng – PersonEntity: Name: NameFull: Zhang, Ming – PersonEntity: Name: NameFull: Zhao, Xiaodong IsPartOfRelationships: – BibEntity: Dates: – D: 21 M: 12 Text: 12/21/2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 16648021 Numbering: – Type: volume Value: 12 Titles: – TitleFull: Frontiers in Genetics Type: main |
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