Direct capillary electrophoresis analysis of basic and acidic drugs from microliter volume of human body fluids after liquid-phase microextraction through nano-fibrous membrane.
| Title: | Direct capillary electrophoresis analysis of basic and acidic drugs from microliter volume of human body fluids after liquid-phase microextraction through nano-fibrous membrane. |
|---|---|
| Authors: | Román-Hidalgo, Cristina, Dvořák, Miloš, Kubáň, Pavel, Martín-Valero, María Jesús, Bello-López, Miguel Ángel |
| Source: | Analytical & Bioanalytical Chemistry; Jan2020, Vol. 412 Issue 1, p181-191, 11p |
| Subject Terms: | HUMAN body, CAPILLARY electrophoresis, BODY fluid analysis, BODY fluids, LIQUID membranes, DRUGS, POLYAMIDES, NONSTEROIDAL anti-inflammatory agents |
| Abstract: | In the present work, a disposable microextraction device with a polyamide 6 nano-fibrous supported liquid membrane (SLM) is employed for the pretreatment of minute volumes of biological fluids. The device is placed in a sample vial for an at-line coupling to a commercial capillary electrophoresis instrument with UV-Vis detection (CE-UV) and injections are performed fully automatically from the free acceptor solution above the SLM with no contact between the capillary and the membrane. Up to 4-fold enrichment of model basic (nortriptyline, haloperidol, loperamide, and papaverine) and acidic (ibuprofen, naproxen, ketoprofen, and diclofenac) drugs is achieved by optimizing the ratio of the donor to the acceptor solution volumes (16 to 4 μL, respectively). The actual setup enables SLM extractions from less than a drop of sample and is suitable for pretreatment of scarce human body fluids. Two unique methods are reported for efficient clean-up and enrichment of the basic and acidic drugs from capillary blood (formed as dried blood spot), serum, and urine samples, which enable their determination at therapeutic and/or toxic levels. The hyphenation of the SLM extraction with CE-UV analysis provides good repeatability (RSD, 2.4–14.9%), linearity (r2, 0.988–1.000), sensitivity (LOD, 0.017–0.22 mg L−1), and extraction recovery (ER, 20–106%) at short extraction times (10 min) and with minimum consumption of samples and reagents. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Direct capillary electrophoresis analysis of basic and acidic drugs from microliter volume of human body fluids after liquid-phase microextraction through nano-fibrous membrane. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Román-Hidalgo%2C+Cristina%22">Román-Hidalgo, Cristina</searchLink><br /><searchLink fieldCode="AR" term="%22Dvořák%2C+Miloš%22">Dvořák, Miloš</searchLink><br /><searchLink fieldCode="AR" term="%22Kubáň%2C+Pavel%22">Kubáň, Pavel</searchLink><br /><searchLink fieldCode="AR" term="%22Martín-Valero%2C+María+Jesús%22">Martín-Valero, María Jesús</searchLink><br /><searchLink fieldCode="AR" term="%22Bello-López%2C+Miguel+Ángel%22">Bello-López, Miguel Ángel</searchLink> – Name: TitleSource Label: Source Group: Src Data: Analytical & Bioanalytical Chemistry; Jan2020, Vol. 412 Issue 1, p181-191, 11p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22HUMAN+body%22">HUMAN body</searchLink><br /><searchLink fieldCode="DE" term="%22CAPILLARY+electrophoresis%22">CAPILLARY electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22BODY+fluid+analysis%22">BODY fluid analysis</searchLink><br /><searchLink fieldCode="DE" term="%22BODY+fluids%22">BODY fluids</searchLink><br /><searchLink fieldCode="DE" term="%22LIQUID+membranes%22">LIQUID membranes</searchLink><br /><searchLink fieldCode="DE" term="%22DRUGS%22">DRUGS</searchLink><br /><searchLink fieldCode="DE" term="%22POLYAMIDES%22">POLYAMIDES</searchLink><br /><searchLink fieldCode="DE" term="%22NONSTEROIDAL+anti-inflammatory+agents%22">NONSTEROIDAL anti-inflammatory agents</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In the present work, a disposable microextraction device with a polyamide 6 nano-fibrous supported liquid membrane (SLM) is employed for the pretreatment of minute volumes of biological fluids. The device is placed in a sample vial for an at-line coupling to a commercial capillary electrophoresis instrument with UV-Vis detection (CE-UV) and injections are performed fully automatically from the free acceptor solution above the SLM with no contact between the capillary and the membrane. Up to 4-fold enrichment of model basic (nortriptyline, haloperidol, loperamide, and papaverine) and acidic (ibuprofen, naproxen, ketoprofen, and diclofenac) drugs is achieved by optimizing the ratio of the donor to the acceptor solution volumes (16 to 4 μL, respectively). The actual setup enables SLM extractions from less than a drop of sample and is suitable for pretreatment of scarce human body fluids. Two unique methods are reported for efficient clean-up and enrichment of the basic and acidic drugs from capillary blood (formed as dried blood spot), serum, and urine samples, which enable their determination at therapeutic and/or toxic levels. The hyphenation of the SLM extraction with CE-UV analysis provides good repeatability (RSD, 2.4–14.9%), linearity (r<superscript>2</superscript>, 0.988–1.000), sensitivity (LOD, 0.017–0.22 mg L<superscript>−1</superscript>), and extraction recovery (ER, 20–106%) at short extraction times (10 min) and with minimum consumption of samples and reagents. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Analytical & Bioanalytical Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00216-019-02225-y Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 181 Subjects: – SubjectFull: HUMAN body Type: general – SubjectFull: CAPILLARY electrophoresis Type: general – SubjectFull: BODY fluid analysis Type: general – SubjectFull: BODY fluids Type: general – SubjectFull: LIQUID membranes Type: general – SubjectFull: DRUGS Type: general – SubjectFull: POLYAMIDES Type: general – SubjectFull: NONSTEROIDAL anti-inflammatory agents Type: general Titles: – TitleFull: Direct capillary electrophoresis analysis of basic and acidic drugs from microliter volume of human body fluids after liquid-phase microextraction through nano-fibrous membrane. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Román-Hidalgo, Cristina – PersonEntity: Name: NameFull: Dvořák, Miloš – PersonEntity: Name: NameFull: Kubáň, Pavel – PersonEntity: Name: NameFull: Martín-Valero, María Jesús – PersonEntity: Name: NameFull: Bello-López, Miguel Ángel IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 16182642 Numbering: – Type: volume Value: 412 – Type: issue Value: 1 Titles: – TitleFull: Analytical & Bioanalytical Chemistry Type: main |
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