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Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to Indica Rice.

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Title: Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to Indica Rice.
Authors: Verma, Rakesh Kumar, Santosh Kumar, Vinjamuri Venkata, Yadav, Shashank Kumar, Pushkar, Suchitra, Rao, Mandali Venkateswara, Chinnusamy, Viswanathan
Source: Frontiers in Plant Science; 11/28/2019, Vol. 10, p1-16, 16p
Subject Terms: DROUGHT tolerance, ABSCISIC acid, GENETIC transformation, RICE, GRAIN yields, TRANSGENIC plants, WATER analysis
Abstract: Abscisic acid (ABA) plays versatile functions in regulating plant development and tolerance to various biotic and abiotic stresses. Towards elucidating the functions of one of the ABA receptors (ABARs) in rice, OsPYL10 was cloned from drought tolerant rice cv. Nagina 22 and was overexpressed under stress inducible RD29A promoter in a mega rice variety MTU1010 by using Agrobacterium mediated genetic transformation. Four single copy transgenic lines selected based on Southern blot analysis were used for physiological and molecular analysis. PYL10 receptor appears to regulate its ligand ABA accumulation as PYL10 overexpressing transgenics accumulated 2–3.3-fold higher levels of ABA than that of WT in flag leaf at anthesis under non-stress conditions. The enhanced accumulation of ABA was associated with enhanced expression of genes for ABA biosynthesis viz., ZEP1 , NCED1 , NCED2 , NCED3 , and NCED4 in transgenics than in WT plants. At seedling stage, PYL10 transgenics showed significantly higher survival rate under cold stress as compared with WT plants. qRT-PCR analysis showed that expression levels of cold responsive genes viz., DREB1F , MYB3R2 , TPP1 , COR410 , DEHYDRIN , and LEA3 were significantly higher in PYL10 overexpressing transgenic lines as compared to WT plants under cold stress. PYL10 transgenic and WT plants grown in the same pot were subjected to -80 kPa drought stress and recovery treatments at vegetative and reproductive stages. At vegetative stage drought stress, three overexpressing lines showed significantly higher grain yield (40–58%) and at reproductive stage drought stress one of these overexpression lines showed two-fold higher grain yield than that of WT plants. Excised leaf water loss analysis showed that PYL10 transgenic lost about 20% less water than WT plants. At reproductive stage, OsPYL10 transgenic maintained higher RWC, membrane stability index, chlorophyll content, and accumulated lower amount of MDA and H2O2 as compared with WT plants. qRT-PCR analysis showed that expression levels of RAB16 , Dehydrin , LEA3 , and ABA45 were higher in PYL10 transgenics as compared with WT plants under drought stress. Thus, overall results showed that OsPYL10 overexpression has potential to improve both drought and cold stress tolerance of indica rice. [ABSTRACT FROM AUTHOR]
Copyright of Frontiers in Plant Science is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to Indica Rice.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Verma%2C+Rakesh+Kumar%22">Verma, Rakesh Kumar</searchLink><br /><searchLink fieldCode="AR" term="%22Santosh+Kumar%2C+Vinjamuri+Venkata%22">Santosh Kumar, Vinjamuri Venkata</searchLink><br /><searchLink fieldCode="AR" term="%22Yadav%2C+Shashank+Kumar%22">Yadav, Shashank Kumar</searchLink><br /><searchLink fieldCode="AR" term="%22Pushkar%2C+Suchitra%22">Pushkar, Suchitra</searchLink><br /><searchLink fieldCode="AR" term="%22Rao%2C+Mandali+Venkateswara%22">Rao, Mandali Venkateswara</searchLink><br /><searchLink fieldCode="AR" term="%22Chinnusamy%2C+Viswanathan%22">Chinnusamy, Viswanathan</searchLink>
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  Label: Source
  Group: Src
  Data: Frontiers in Plant Science; 11/28/2019, Vol. 10, p1-16, 16p
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22DROUGHT+tolerance%22">DROUGHT tolerance</searchLink><br /><searchLink fieldCode="DE" term="%22ABSCISIC+acid%22">ABSCISIC acid</searchLink><br /><searchLink fieldCode="DE" term="%22GENETIC+transformation%22">GENETIC transformation</searchLink><br /><searchLink fieldCode="DE" term="%22RICE%22">RICE</searchLink><br /><searchLink fieldCode="DE" term="%22GRAIN+yields%22">GRAIN yields</searchLink><br /><searchLink fieldCode="DE" term="%22TRANSGENIC+plants%22">TRANSGENIC plants</searchLink><br /><searchLink fieldCode="DE" term="%22WATER+analysis%22">WATER analysis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abscisic acid (ABA) plays versatile functions in regulating plant development and tolerance to various biotic and abiotic stresses. Towards elucidating the functions of one of the ABA receptors (ABARs) in rice, OsPYL10 was cloned from drought tolerant rice cv. Nagina 22 and was overexpressed under stress inducible RD29A promoter in a mega rice variety MTU1010 by using Agrobacterium mediated genetic transformation. Four single copy transgenic lines selected based on Southern blot analysis were used for physiological and molecular analysis. PYL10 receptor appears to regulate its ligand ABA accumulation as PYL10 overexpressing transgenics accumulated 2–3.3-fold higher levels of ABA than that of WT in flag leaf at anthesis under non-stress conditions. The enhanced accumulation of ABA was associated with enhanced expression of genes for ABA biosynthesis viz., ZEP1 , NCED1 , NCED2 , NCED3 , and NCED4 in transgenics than in WT plants. At seedling stage, PYL10 transgenics showed significantly higher survival rate under cold stress as compared with WT plants. qRT-PCR analysis showed that expression levels of cold responsive genes viz., DREB1F , MYB3R2 , TPP1 , COR410 , DEHYDRIN , and LEA3 were significantly higher in PYL10 overexpressing transgenic lines as compared to WT plants under cold stress. PYL10 transgenic and WT plants grown in the same pot were subjected to -80 kPa drought stress and recovery treatments at vegetative and reproductive stages. At vegetative stage drought stress, three overexpressing lines showed significantly higher grain yield (40–58%) and at reproductive stage drought stress one of these overexpression lines showed two-fold higher grain yield than that of WT plants. Excised leaf water loss analysis showed that PYL10 transgenic lost about 20% less water than WT plants. At reproductive stage, OsPYL10 transgenic maintained higher RWC, membrane stability index, chlorophyll content, and accumulated lower amount of MDA and H<subscript>2</subscript>O<subscript>2</subscript> as compared with WT plants. qRT-PCR analysis showed that expression levels of RAB16 , Dehydrin , LEA3 , and ABA45 were higher in PYL10 transgenics as compared with WT plants under drought stress. Thus, overall results showed that OsPYL10 overexpression has potential to improve both drought and cold stress tolerance of indica rice. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Frontiers in Plant Science is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3389/fpls.2019.01488
    Languages:
      – Code: eng
        Text: English
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        PageCount: 16
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    Subjects:
      – SubjectFull: DROUGHT tolerance
        Type: general
      – SubjectFull: ABSCISIC acid
        Type: general
      – SubjectFull: GENETIC transformation
        Type: general
      – SubjectFull: RICE
        Type: general
      – SubjectFull: GRAIN yields
        Type: general
      – SubjectFull: TRANSGENIC plants
        Type: general
      – SubjectFull: WATER analysis
        Type: general
    Titles:
      – TitleFull: Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to Indica Rice.
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            NameFull: Verma, Rakesh Kumar
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            NameFull: Santosh Kumar, Vinjamuri Venkata
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            NameFull: Yadav, Shashank Kumar
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            NameFull: Pushkar, Suchitra
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            NameFull: Rao, Mandali Venkateswara
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            NameFull: Chinnusamy, Viswanathan
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              Text: 11/28/2019
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              Y: 2019
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