Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro.
| Title: | Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. |
|---|---|
| Authors: | ZHAOXIA ZHANG, TIANYOU WANG, ZIQIN LIU, SUOQIN TANG, MEI YUE, SHUNQIAO FENG, MENGZE HU, LITIAN XUAN, YANFEI CHEN |
| Source: | Experimental & Therapeutic Medicine; Jul2017, Vol. 14 Issue 1, p35-42, 8p |
| Subject Terms: | SMALL interfering RNA, TUMORS, CELL proliferation, CELL division, PROPIDIUM iodide |
| Abstract: | The present study aimed to evaluate the impact of small interfering RNA (siRNA) targeting of the survivin gene in human tumor cells and the effect of decreased survivin expression on the proliferation and apoptosis of tumor cells. Human tumor cell lines (MSA-MB-231, SGC-7901, HeLa, A549, SK-OV-3 and Raji, PC-3) were cultured in vitro and divided into three groups: survivin siRNA-treated, scrambled negative control siRNA-treated and an untreated control group. The level of survivin mRNA and protein expression was subsequently determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. Cell proliferation was also examined by an MTT assay following transfection and the apoptotic rate of cells was detected by Hoechst and Annexin V/propidium iodide staining. It was observed that relative to the control group, expression of survivin mRNA and protein in the survivin siRNA-treated group was significantly downregulated. Furthermore, siRNA targeting of survivin lead to the inhibition of tumor cell proliferation, as well as an increase in their apoptotic rate in vitro. These data suggest that survivin may be a potential tumor biomarker and a novel target for the treatment of cancer. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Header | DbId: edb DbLabel: Complementary Index An: 123875823 RelevancyScore: 873 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 873.165649414063 |
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| Items | – Name: Title Label: Title Group: Ti Data: Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22ZHAOXIA+ZHANG%22">ZHAOXIA ZHANG</searchLink><br /><searchLink fieldCode="AR" term="%22TIANYOU+WANG%22">TIANYOU WANG</searchLink><br /><searchLink fieldCode="AR" term="%22ZIQIN+LIU%22">ZIQIN LIU</searchLink><br /><searchLink fieldCode="AR" term="%22SUOQIN+TANG%22">SUOQIN TANG</searchLink><br /><searchLink fieldCode="AR" term="%22MEI+YUE%22">MEI YUE</searchLink><br /><searchLink fieldCode="AR" term="%22SHUNQIAO+FENG%22">SHUNQIAO FENG</searchLink><br /><searchLink fieldCode="AR" term="%22MENGZE+HU%22">MENGZE HU</searchLink><br /><searchLink fieldCode="AR" term="%22LITIAN+XUAN%22">LITIAN XUAN</searchLink><br /><searchLink fieldCode="AR" term="%22YANFEI+CHEN%22">YANFEI CHEN</searchLink> – Name: TitleSource Label: Source Group: Src Data: Experimental & Therapeutic Medicine; Jul2017, Vol. 14 Issue 1, p35-42, 8p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22SMALL+interfering+RNA%22">SMALL interfering RNA</searchLink><br /><searchLink fieldCode="DE" term="%22TUMORS%22">TUMORS</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+proliferation%22">CELL proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+division%22">CELL division</searchLink><br /><searchLink fieldCode="DE" term="%22PROPIDIUM+iodide%22">PROPIDIUM iodide</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The present study aimed to evaluate the impact of small interfering RNA (siRNA) targeting of the survivin gene in human tumor cells and the effect of decreased survivin expression on the proliferation and apoptosis of tumor cells. Human tumor cell lines (MSA-MB-231, SGC-7901, HeLa, A549, SK-OV-3 and Raji, PC-3) were cultured in vitro and divided into three groups: survivin siRNA-treated, scrambled negative control siRNA-treated and an untreated control group. The level of survivin mRNA and protein expression was subsequently determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. Cell proliferation was also examined by an MTT assay following transfection and the apoptotic rate of cells was detected by Hoechst and Annexin V/propidium iodide staining. It was observed that relative to the control group, expression of survivin mRNA and protein in the survivin siRNA-treated group was significantly downregulated. Furthermore, siRNA targeting of survivin lead to the inhibition of tumor cell proliferation, as well as an increase in their apoptotic rate in vitro. These data suggest that survivin may be a potential tumor biomarker and a novel target for the treatment of cancer. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Experimental & Therapeutic Medicine is the property of Spandidos Publications UK Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3892/etm.2017.4501 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 35 Subjects: – SubjectFull: SMALL interfering RNA Type: general – SubjectFull: TUMORS Type: general – SubjectFull: CELL proliferation Type: general – SubjectFull: CELL division Type: general – SubjectFull: PROPIDIUM iodide Type: general Titles: – TitleFull: Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: ZHAOXIA ZHANG – PersonEntity: Name: NameFull: TIANYOU WANG – PersonEntity: Name: NameFull: ZIQIN LIU – PersonEntity: Name: NameFull: SUOQIN TANG – PersonEntity: Name: NameFull: MEI YUE – PersonEntity: Name: NameFull: SHUNQIAO FENG – PersonEntity: Name: NameFull: MENGZE HU – PersonEntity: Name: NameFull: LITIAN XUAN – PersonEntity: Name: NameFull: YANFEI CHEN IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 17920981 Numbering: – Type: volume Value: 14 – Type: issue Value: 1 Titles: – TitleFull: Experimental & Therapeutic Medicine Type: main |
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