Tetrodotoxin and paralytic shellfish poisons in gastropod species from Vietnam analyzed by high-performance liquid chromatography and liquid chromatography--tandem mass spectrometry.
| Title: | Tetrodotoxin and paralytic shellfish poisons in gastropod species from Vietnam analyzed by high-performance liquid chromatography and liquid chromatography--tandem mass spectrometry. |
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| Authors: | Hsiao-Chin Jen1, Thi Anh-Tuyet Nguyen2, Ya-Jung Wu3, Tung Hoang2, Osamu Arakawa4, Wen-Feng Lin3, Deng-Fwu Hwang3 dfhwang@mail.ntou.edu.tw |
| Source: | Journal of Food & Drug Analysis. Jun2014, Vol. 22 Issue 2, p178-188. 11p. |
| Subject Terms: | *ANIMAL experimentation, *HIGH performance liquid chromatography, *MARINE toxins, *MASS spectrometry, *MOLLUSKS, *NEUROTOXICOLOGY, *RESEARCH funding, *SHELLFISH, *SYNDROMES |
| Geographic Terms: | VIETNAM |
| Abstract: | Among marine toxins, tetrodotoxin (TTX) and paralytic shellfish poisons (PSPs) are known as notorious neurotoxins that induce serious food poisoning incidents in the Southeast Asia region. The aim of this study was to investigate whether TTX and PSP toxins are important issues of seafood safety. Paralytic toxicity was observed in mice exposed to 34 specimens from five species of gastropods using a PSP bioassay. Five species of gastropods, Natica vitellus, Natica tumidus, Oliva hirasei, Oliva lignaria, and Oliva annulata, were collected from the coastal seawaters in Nha Trang City, Vietnam, between August 2007 and October 2007. The average lethal potency of gastropod specimens was 90 ± 40 (mean ± standard deviation) mouse units (MU) for N. vitellus, 64 ± 19 MU for N. tumidus, 42 ± 28 MU for O. hirasei, 51 ± 17 MU for O. lignaria, and 39 ± 18 MU for O. annulata. All toxic extracts from the sample species were clarified using a C18 Sep-Pak solid-phase extraction column and a microcentrifuge filter prior to analysis. High-performance liquid chromatography coupled with fluorescence detection indicated that the toxins of the olive shell (O. hirasei, O. lignaria, and O. annulata) were mainly composed of saxitoxin (STX) (73–82%), gonyautoxin (GTX) 2, 3 (12–22%), and minor levels of TTX (5–6%). The toxins of N. vitellus and N. tumidus were mainly composed of STX (76–81%) and GTX 1, 4 (19–24%). Furthermore, liquid chromatography–tandem mass spectrometry analysis was used to verify the identity of the PSPs and TTX. Our evidence shows that these gastropods have novel toxin profiles. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Tetrodotoxin and paralytic shellfish poisons in gastropod species from Vietnam analyzed by high-performance liquid chromatography and liquid chromatography--tandem mass spectrometry. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Hsiao-Chin+Jen%22">Hsiao-Chin Jen</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Thi+Anh-Tuyet+Nguyen%22">Thi Anh-Tuyet Nguyen</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ya-Jung+Wu%22">Ya-Jung Wu</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Tung+Hoang%22">Tung Hoang</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Osamu+Arakawa%22">Osamu Arakawa</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Wen-Feng+Lin%22">Wen-Feng Lin</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Deng-Fwu+Hwang%22">Deng-Fwu Hwang</searchLink><relatesTo>3</relatesTo><i> dfhwang@mail.ntou.edu.tw</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+%26+Drug+Analysis%22">Journal of Food & Drug Analysis</searchLink>. Jun2014, Vol. 22 Issue 2, p178-188. 11p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22ANIMAL+experimentation%22">ANIMAL experimentation</searchLink><br />*<searchLink fieldCode="DE" term="%22HIGH+performance+liquid+chromatography%22">HIGH performance liquid chromatography</searchLink><br />*<searchLink fieldCode="DE" term="%22MARINE+toxins%22">MARINE toxins</searchLink><br />*<searchLink fieldCode="DE" term="%22MASS+spectrometry%22">MASS spectrometry</searchLink><br />*<searchLink fieldCode="DE" term="%22MOLLUSKS%22">MOLLUSKS</searchLink><br />*<searchLink fieldCode="DE" term="%22NEUROTOXICOLOGY%22">NEUROTOXICOLOGY</searchLink><br />*<searchLink fieldCode="DE" term="%22RESEARCH+funding%22">RESEARCH funding</searchLink><br />*<searchLink fieldCode="DE" term="%22SHELLFISH%22">SHELLFISH</searchLink><br />*<searchLink fieldCode="DE" term="%22SYNDROMES%22">SYNDROMES</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22VIETNAM%22">VIETNAM</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Among marine toxins, tetrodotoxin (TTX) and paralytic shellfish poisons (PSPs) are known as notorious neurotoxins that induce serious food poisoning incidents in the Southeast Asia region. The aim of this study was to investigate whether TTX and PSP toxins are important issues of seafood safety. Paralytic toxicity was observed in mice exposed to 34 specimens from five species of gastropods using a PSP bioassay. Five species of gastropods, Natica vitellus, Natica tumidus, Oliva hirasei, Oliva lignaria, and Oliva annulata, were collected from the coastal seawaters in Nha Trang City, Vietnam, between August 2007 and October 2007. The average lethal potency of gastropod specimens was 90 ± 40 (mean ± standard deviation) mouse units (MU) for N. vitellus, 64 ± 19 MU for N. tumidus, 42 ± 28 MU for O. hirasei, 51 ± 17 MU for O. lignaria, and 39 ± 18 MU for O. annulata. All toxic extracts from the sample species were clarified using a C18 Sep-Pak solid-phase extraction column and a microcentrifuge filter prior to analysis. High-performance liquid chromatography coupled with fluorescence detection indicated that the toxins of the olive shell (O. hirasei, O. lignaria, and O. annulata) were mainly composed of saxitoxin (STX) (73–82%), gonyautoxin (GTX) 2, 3 (12–22%), and minor levels of TTX (5–6%). The toxins of N. vitellus and N. tumidus were mainly composed of STX (76–81%) and GTX 1, 4 (19–24%). Furthermore, liquid chromatography–tandem mass spectrometry analysis was used to verify the identity of the PSPs and TTX. Our evidence shows that these gastropods have novel toxin profiles. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Food & Drug Analysis is the property of Bureau of Food & Drug Analysis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jfda.2013.09.005 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 178 Subjects: – SubjectFull: VIETNAM Type: general – SubjectFull: ANIMAL experimentation Type: general – SubjectFull: HIGH performance liquid chromatography Type: general – SubjectFull: MARINE toxins Type: general – SubjectFull: MASS spectrometry Type: general – SubjectFull: MOLLUSKS Type: general – SubjectFull: NEUROTOXICOLOGY Type: general – SubjectFull: RESEARCH funding Type: general – SubjectFull: SHELLFISH Type: general – SubjectFull: SYNDROMES Type: general Titles: – TitleFull: Tetrodotoxin and paralytic shellfish poisons in gastropod species from Vietnam analyzed by high-performance liquid chromatography and liquid chromatography--tandem mass spectrometry. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hsiao-Chin Jen – PersonEntity: Name: NameFull: Thi Anh-Tuyet Nguyen – PersonEntity: Name: NameFull: Ya-Jung Wu – PersonEntity: Name: NameFull: Tung Hoang – PersonEntity: Name: NameFull: Osamu Arakawa – PersonEntity: Name: NameFull: Wen-Feng Lin – PersonEntity: Name: NameFull: Deng-Fwu Hwang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 10219498 Numbering: – Type: volume Value: 22 – Type: issue Value: 2 Titles: – TitleFull: Journal of Food & Drug Analysis Type: main |
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