Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection.
| Title: | Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection. |
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| Authors: | Jiezuan Yang1,2, Jianqin He1,2, Haijun Huang1,2,3, Zhongkang Ji1,2, Li Wei1,2, Ping Ye1,2, Kaijin Xu1,2, Lanjuan Li ljli@zju.edu.cn |
| Source: | BMC Infectious Diseases. 2013, Vol. 13 Issue 1, p1-10. 10p. 5 Charts, 3 Graphs. |
| Subject Terms: | *T cell receptors, *TUBERCULOSIS, *MYCOBACTERIUM, *LYMPHOCYTES, *MONONUCLEAR leukocytes |
| Abstract: | Background: T cells are closely linked to the clinical manifestations of subjects with Mycobacterium tuberculosis (MTB) infection. T cell receptor beta variable (TCRBV) is a signal and indicative molecule on the membrane of T lymphocytes, reflecting the composition and specificity of T cells. The molecular profiles of TCRBV in peripheral blood mononuclear cells (PBMCs) and their subpopulations (CD4+ and CD8+ T cells) from subjects with active tuberculosis (TB) or latent TB infection (LTBI) have not been well described. Methods: In 42 subjects with active TB or LTBI, PMBCs and their subsets were separated and sorted. The molecular profiles of the TCRBV complementarity determining region 3 (CDR3) in the three cell populations were investigated using our recently developed gene melting spectral pattern (GMSP) assay. The TCRBV members were then cloned and sequenced when their GMSP image profiles showed a single-peak. Results: The average number of skewed TCRBV molecules in the CD4+ cell subset was significantly higher than that in PBMCs and CD8+ T cells. TCRBV12, BV13.1, BV13.2, and BV24 were expressed more prevalently than other TCRBV gene families in the three cell populations. In addition, relatively conserved amino acid motifs were identified in TCRBV5.1 and BV20 CDR3 in PBMCs and its subsets. The monoclonal TCRBV14 and BV23 expressed were different between active TB and LTBI subjects. Conclusions: These results indicate that the T cell immune response is complex and multi-specific in active TB and LTBI subjects. Analysis of TCRBV expression in CD4+ T cells suggest that it could be useful in assessing the composition and status of circulating T cells. Furthermore, the expression of TCRBV14, BV23 and the sequencing of CDR3 amino acid motifs of TCRBV5.1, BV20 could be used in the differential diagnosis and treatment of subjects with active TB or LTBI. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Jiezuan+Yang%22">Jiezuan Yang</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Jianqin+He%22">Jianqin He</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Haijun+Huang%22">Haijun Huang</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhongkang+Ji%22">Zhongkang Ji</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Li+Wei%22">Li Wei</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ping+Ye%22">Ping Ye</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kaijin+Xu%22">Kaijin Xu</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lanjuan+Li%22">Lanjuan Li</searchLink><i> ljli@zju.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22BMC+Infectious+Diseases%22">BMC Infectious Diseases</searchLink>. 2013, Vol. 13 Issue 1, p1-10. 10p. 5 Charts, 3 Graphs. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22T+cell+receptors%22">T cell receptors</searchLink><br />*<searchLink fieldCode="DE" term="%22TUBERCULOSIS%22">TUBERCULOSIS</searchLink><br />*<searchLink fieldCode="DE" term="%22MYCOBACTERIUM%22">MYCOBACTERIUM</searchLink><br />*<searchLink fieldCode="DE" term="%22LYMPHOCYTES%22">LYMPHOCYTES</searchLink><br />*<searchLink fieldCode="DE" term="%22MONONUCLEAR+leukocytes%22">MONONUCLEAR leukocytes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background: T cells are closely linked to the clinical manifestations of subjects with Mycobacterium tuberculosis (MTB) infection. T cell receptor beta variable (TCRBV) is a signal and indicative molecule on the membrane of T lymphocytes, reflecting the composition and specificity of T cells. The molecular profiles of TCRBV in peripheral blood mononuclear cells (PBMCs) and their subpopulations (CD4+ and CD8+ T cells) from subjects with active tuberculosis (TB) or latent TB infection (LTBI) have not been well described. Methods: In 42 subjects with active TB or LTBI, PMBCs and their subsets were separated and sorted. The molecular profiles of the TCRBV complementarity determining region 3 (CDR3) in the three cell populations were investigated using our recently developed gene melting spectral pattern (GMSP) assay. The TCRBV members were then cloned and sequenced when their GMSP image profiles showed a single-peak. Results: The average number of skewed TCRBV molecules in the CD4+ cell subset was significantly higher than that in PBMCs and CD8+ T cells. TCRBV12, BV13.1, BV13.2, and BV24 were expressed more prevalently than other TCRBV gene families in the three cell populations. In addition, relatively conserved amino acid motifs were identified in TCRBV5.1 and BV20 CDR3 in PBMCs and its subsets. The monoclonal TCRBV14 and BV23 expressed were different between active TB and LTBI subjects. Conclusions: These results indicate that the T cell immune response is complex and multi-specific in active TB and LTBI subjects. Analysis of TCRBV expression in CD4+ T cells suggest that it could be useful in assessing the composition and status of circulating T cells. Furthermore, the expression of TCRBV14, BV23 and the sequencing of CDR3 amino acid motifs of TCRBV5.1, BV20 could be used in the differential diagnosis and treatment of subjects with active TB or LTBI. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of BMC Infectious Diseases is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1186/1471-2334-13-423 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 1 Subjects: – SubjectFull: T cell receptors Type: general – SubjectFull: TUBERCULOSIS Type: general – SubjectFull: MYCOBACTERIUM Type: general – SubjectFull: LYMPHOCYTES Type: general – SubjectFull: MONONUCLEAR leukocytes Type: general Titles: – TitleFull: Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Jiezuan Yang – PersonEntity: Name: NameFull: Jianqin He – PersonEntity: Name: NameFull: Haijun Huang – PersonEntity: Name: NameFull: Zhongkang Ji – PersonEntity: Name: NameFull: Li Wei – PersonEntity: Name: NameFull: Ping Ye – PersonEntity: Name: NameFull: Kaijin Xu – PersonEntity: Name: NameFull: Lanjuan Li IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: 2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 14712334 Numbering: – Type: volume Value: 13 – Type: issue Value: 1 Titles: – TitleFull: BMC Infectious Diseases Type: main |
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