Monitoramento por imagem de ressonância magnética do crescimento tumoral no modelo C6 de glioblastoma com perspectivas de avaliação da terapia de magnetohipertemia.
| Title: | Monitoramento por imagem de ressonância magnética do crescimento tumoral no modelo C6 de glioblastoma com perspectivas de avaliação da terapia de magnetohipertemia. |
|---|---|
| Alternate Title: | Tumor growth analysis by magnetic resonance image of the C6 glioblastoma model with prospects for the assessment of magnetohypertemia therapy. |
| Authors: | da Silva, André César1, Cabral, Francisco Romero1, Mamani, Javier Bustamante1, Malheiros, Jackeline Moraes2, Polli, Roberson Saraiva2, Tannus, Alberto2, Vidoto, Edson2, Martins, Mateus José2, Sibov, Tatiana Tais1, Pavon, Lorena Favaro1, Miyaki, Liza Aya Mabuchi1, Cárdenas, Walter Humberto Zavala1, Malheiros, Suzana Maria Fleury3, Brandt, Reynaldo André4, Junior, Edson Amaro1, Gamarra, Lionel Fernel1 lgamarra@einstein.br |
| Source: | Einstein (16794508). Jan-Mar2012, Vol. 10 Issue 1, p11-15. 5p. 1 Color Photograph, 1 Black and White Photograph. |
| Subject Terms: | *TUMOR growth, *GLIOBLASTOMA multiforme, *MAGNETIC resonance imaging, *LABORATORY rats, *ONCOGENIC viruses, *ONCOLOGY |
| Abstract (English): | Objective: The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy. Methods: Young male Wistar rats weighing between 250 and 300 g were used for the C6 model. After the rates were anesthetized (55 mg/ kg ketamine and 11 mg/kg xylazine), C6 lineage tumorigenic cells suspended in culture medium (105 cells in 10 μl) were stereotaxically injected into the right frontal cortex (bregma coordinates: 2.0 mm anteroposterior, 3.0 mm laterolateral, and 2.5 mm depth) of the rats using a Hamilton syringe. For the control group, the rats were injected with culture medium without cells. MRI scans were performed at 14, 21, and 28 d after the injection using a 2.0 T MRI scanner (Bruker BioSpec, Germany). The animals were anesthetized with 55 mg/kg ketamine and 11 mg/kg xylazine before being examined. Coronal multilayers were acquired using a standard spin echo sequence with the following parameters: repetition/echo time = 4.000 ms/67.1 ms, field of view = 3.50, matrix = 192, slice thickness = 0.4 mm, and slice separation = 0 mm. Results: The MRI analysis enabled a clear visualization of the tumor mass, and it was possible to establish the tumor volume parameters on the various days that were examined. The volume at 14 d after induction was 13.7 ± 2.5 mm3. On days 21 and 28, the tumor volumes were 31.7 ± 6.5 mm3 and 122.1 ± 11.8 mm3, respectively. Conclusion: These results demonstrated that it is possible to evaluate the C6 model tumor volume in rats, which will allow for the future implementation and verification of magnetic hyperthermia therapy. [ABSTRACT FROM AUTHOR] |
| Abstract (Portuguese): | Objetivo: Estabelecer um padrão de crescimento tumoral (volume) em ratos Wistar submetidos ao modelo C6 de glioblastoma multiforme por meio de imagens de ressonância magnética para posterior verificação de redução de volume tumoral com a terapia de magnetohipertermia. Métodos: Para o modelo C6, utilizamos ratos Wistar, machos, jovens, pesando entre 250 e 300 g. Após anestesiados (cetamina 55 mg/kg e xilazina 11 mg/kg) foram injetadas estereotaxicamente células tumorigênicas linhagem C6 suspensas em meio de cultura (105 células em 10 μL) no córtex frontal direito (coordenadas a partir do bregma: anteroposterior = 2,0 mm; látero-lateral = 3,0 mm; profundidade = 2,5 mm) com uma seringa Hamilton. No Grupo Controle, houve a injeção do meio de cultura sem as células. Posteriormente, foram feitas imagens mediante a técnica de imagem por ressonância magnética em 14, 21 e 28 dias após a injeção em um escâner de imagem por ressonância magnética 2.0 T (Bruker BioSpec, Germany). Para o exame, os animais foram anestesiados com cetamina 55 mg/kg e xilazina 11 mg/kg. Multifatias coronais foram adquiridas utilizando uma sequência spin-echo padrão com os seguintes parâmetros: TR/TE = 4,000 ms/67,1 ms, FOV = 3,50, Matrix 192, slice thickness = 0,4 mm e slice separation = 0 mm. Resultados: A análise das imagens de ressonância magnética do tumor possibilitou a clara visualização da massa tumoral, sendo possível ainda estabelecer parâmetros de volume tumoral nos diferentes dias analisados. O volume de 14 dias após a indução do foi de 13,7 ± 2,5 mm3. Aos 21 dias, o volume alcançado foi de 31,7 ± 6,5 mm3 e, aos 28 dias, a massa tumoral atingiu 122,1 ± 11,8 mm3. Conclusão: Estes resultados mostraram a possiblidade de avaliação do volume tumoral no modelo C6 em ratos, o que possibilitará, no futuro, a aplicação da terapia de magnetohipertermia bem como verificação de seus resultados. [ABSTRACT FROM AUTHOR] |
| Copyright of Einstein (16794508) is the property of Instituto Israelita de Ensino e Pesquisa Albert Einstein and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Academic Search Complete |
| FullText | Links: – Type: pdflink Text: Availability: 0 CustomLinks: – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:a9h&genre=article&issn=16794508&ISBN=&volume=10&issue=1&date=20120101&spage=11&pages=11-15&title=Einstein (16794508)&atitle=Monitoramento%20por%20imagem%20de%20resson%C3%A2ncia%20magn%C3%A9tica%20do%20crescimento%20tumoral%20no%20modelo%20C6%20de%20glioblastoma%20com%20perspectivas%20de%20avalia%C3%A7%C3%A3o%20da%20terapia%20de%20magnetohipertemia.&aulast=da%20Silva%2C%20Andr%C3%A9%20C%C3%A9sar&id=DOI: Name: Full Text Finder (for New FTF UI) (s8985755) Category: fullText Text: Find It @ SCU Libraries MouseOverText: Find It @ SCU Libraries |
|---|---|
| Header | DbId: a9h DbLabel: Academic Search Complete An: 75332019 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Monitoramento por imagem de ressonância magnética do crescimento tumoral no modelo C6 de glioblastoma com perspectivas de avaliação da terapia de magnetohipertemia. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: Tumor growth analysis by magnetic resonance image of the C6 glioblastoma model with prospects for the assessment of magnetohypertemia therapy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22da+Silva%2C+André+César%22">da Silva, André César</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cabral%2C+Francisco+Romero%22">Cabral, Francisco Romero</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mamani%2C+Javier+Bustamante%22">Mamani, Javier Bustamante</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Malheiros%2C+Jackeline+Moraes%22">Malheiros, Jackeline Moraes</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Polli%2C+Roberson+Saraiva%22">Polli, Roberson Saraiva</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Tannus%2C+Alberto%22">Tannus, Alberto</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Vidoto%2C+Edson%22">Vidoto, Edson</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Martins%2C+Mateus+José%22">Martins, Mateus José</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sibov%2C+Tatiana+Tais%22">Sibov, Tatiana Tais</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pavon%2C+Lorena+Favaro%22">Pavon, Lorena Favaro</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Miyaki%2C+Liza+Aya+Mabuchi%22">Miyaki, Liza Aya Mabuchi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cárdenas%2C+Walter+Humberto+Zavala%22">Cárdenas, Walter Humberto Zavala</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Malheiros%2C+Suzana+Maria+Fleury%22">Malheiros, Suzana Maria Fleury</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Brandt%2C+Reynaldo+André%22">Brandt, Reynaldo André</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Junior%2C+Edson+Amaro%22">Junior, Edson Amaro</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gamarra%2C+Lionel+Fernel%22">Gamarra, Lionel Fernel</searchLink><relatesTo>1</relatesTo><i> lgamarra@einstein.br</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Einstein+%2816794508%29%22">Einstein (16794508)</searchLink>. Jan-Mar2012, Vol. 10 Issue 1, p11-15. 5p. 1 Color Photograph, 1 Black and White Photograph. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22TUMOR+growth%22">TUMOR growth</searchLink><br />*<searchLink fieldCode="DE" term="%22GLIOBLASTOMA+multiforme%22">GLIOBLASTOMA multiforme</searchLink><br />*<searchLink fieldCode="DE" term="%22MAGNETIC+resonance+imaging%22">MAGNETIC resonance imaging</searchLink><br />*<searchLink fieldCode="DE" term="%22LABORATORY+rats%22">LABORATORY rats</searchLink><br />*<searchLink fieldCode="DE" term="%22ONCOGENIC+viruses%22">ONCOGENIC viruses</searchLink><br />*<searchLink fieldCode="DE" term="%22ONCOLOGY%22">ONCOLOGY</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: Objective: The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy. Methods: Young male Wistar rats weighing between 250 and 300 g were used for the C6 model. After the rates were anesthetized (55 mg/ kg ketamine and 11 mg/kg xylazine), C6 lineage tumorigenic cells suspended in culture medium (105 cells in 10 μl) were stereotaxically injected into the right frontal cortex (bregma coordinates: 2.0 mm anteroposterior, 3.0 mm laterolateral, and 2.5 mm depth) of the rats using a Hamilton syringe. For the control group, the rats were injected with culture medium without cells. MRI scans were performed at 14, 21, and 28 d after the injection using a 2.0 T MRI scanner (Bruker BioSpec, Germany). The animals were anesthetized with 55 mg/kg ketamine and 11 mg/kg xylazine before being examined. Coronal multilayers were acquired using a standard spin echo sequence with the following parameters: repetition/echo time = 4.000 ms/67.1 ms, field of view = 3.50, matrix = 192, slice thickness = 0.4 mm, and slice separation = 0 mm. Results: The MRI analysis enabled a clear visualization of the tumor mass, and it was possible to establish the tumor volume parameters on the various days that were examined. The volume at 14 d after induction was 13.7 ± 2.5 mm3. On days 21 and 28, the tumor volumes were 31.7 ± 6.5 mm3 and 122.1 ± 11.8 mm3, respectively. Conclusion: These results demonstrated that it is possible to evaluate the C6 model tumor volume in rats, which will allow for the future implementation and verification of magnetic hyperthermia therapy. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Portuguese) Group: Ab Data: Objetivo: Estabelecer um padrão de crescimento tumoral (volume) em ratos Wistar submetidos ao modelo C6 de glioblastoma multiforme por meio de imagens de ressonância magnética para posterior verificação de redução de volume tumoral com a terapia de magnetohipertermia. Métodos: Para o modelo C6, utilizamos ratos Wistar, machos, jovens, pesando entre 250 e 300 g. Após anestesiados (cetamina 55 mg/kg e xilazina 11 mg/kg) foram injetadas estereotaxicamente células tumorigênicas linhagem C6 suspensas em meio de cultura (105 células em 10 μL) no córtex frontal direito (coordenadas a partir do bregma: anteroposterior = 2,0 mm; látero-lateral = 3,0 mm; profundidade = 2,5 mm) com uma seringa Hamilton. No Grupo Controle, houve a injeção do meio de cultura sem as células. Posteriormente, foram feitas imagens mediante a técnica de imagem por ressonância magnética em 14, 21 e 28 dias após a injeção em um escâner de imagem por ressonância magnética 2.0 T (Bruker BioSpec, Germany). Para o exame, os animais foram anestesiados com cetamina 55 mg/kg e xilazina 11 mg/kg. Multifatias coronais foram adquiridas utilizando uma sequência spin-echo padrão com os seguintes parâmetros: TR/TE = 4,000 ms/67,1 ms, FOV = 3,50, Matrix 192, slice thickness = 0,4 mm e slice separation = 0 mm. Resultados: A análise das imagens de ressonância magnética do tumor possibilitou a clara visualização da massa tumoral, sendo possível ainda estabelecer parâmetros de volume tumoral nos diferentes dias analisados. O volume de 14 dias após a indução do foi de 13,7 ± 2,5 mm3. Aos 21 dias, o volume alcançado foi de 31,7 ± 6,5 mm3 e, aos 28 dias, a massa tumoral atingiu 122,1 ± 11,8 mm3. Conclusão: Estes resultados mostraram a possiblidade de avaliação do volume tumoral no modelo C6 em ratos, o que possibilitará, no futuro, a aplicação da terapia de magnetohipertermia bem como verificação de seus resultados. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Einstein (16794508) is the property of Instituto Israelita de Ensino e Pesquisa Albert Einstein and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=a9h&AN=75332019 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: por Text: Portuguese PhysicalDescription: Pagination: PageCount: 5 StartPage: 11 Subjects: – SubjectFull: TUMOR growth Type: general – SubjectFull: GLIOBLASTOMA multiforme Type: general – SubjectFull: MAGNETIC resonance imaging Type: general – SubjectFull: LABORATORY rats Type: general – SubjectFull: ONCOGENIC viruses Type: general – SubjectFull: ONCOLOGY Type: general Titles: – TitleFull: Monitoramento por imagem de ressonância magnética do crescimento tumoral no modelo C6 de glioblastoma com perspectivas de avaliação da terapia de magnetohipertemia. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: da Silva, André César – PersonEntity: Name: NameFull: Cabral, Francisco Romero – PersonEntity: Name: NameFull: Mamani, Javier Bustamante – PersonEntity: Name: NameFull: Malheiros, Jackeline Moraes – PersonEntity: Name: NameFull: Polli, Roberson Saraiva – PersonEntity: Name: NameFull: Tannus, Alberto – PersonEntity: Name: NameFull: Vidoto, Edson – PersonEntity: Name: NameFull: Martins, Mateus José – PersonEntity: Name: NameFull: Sibov, Tatiana Tais – PersonEntity: Name: NameFull: Pavon, Lorena Favaro – PersonEntity: Name: NameFull: Miyaki, Liza Aya Mabuchi – PersonEntity: Name: NameFull: Cárdenas, Walter Humberto Zavala – PersonEntity: Name: NameFull: Malheiros, Suzana Maria Fleury – PersonEntity: Name: NameFull: Brandt, Reynaldo André – PersonEntity: Name: NameFull: Junior, Edson Amaro – PersonEntity: Name: NameFull: Gamarra, Lionel Fernel IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan-Mar2012 Type: published Y: 2012 Identifiers: – Type: issn-print Value: 16794508 Numbering: – Type: volume Value: 10 – Type: issue Value: 1 Titles: – TitleFull: Einstein (16794508) Type: main |
| ResultId | 1 |